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Bovine blood neutrophil acyloxyacyl hydrolase (AOAH) activity during endotoxin and coliform mastitis

(2007) VETERINARY RESEARCH. 38(5). p.655-668
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Abstract
The dynamics of blood neutrophil acyloxyacyl hydrolase ( AOAH) activity, the appearance of endotoxin ( lipopolysaccharide, LPS) in blood and the role of blood neutrophil AOAH in the severity of Escherichia coli and endotoxin mastitis were investigated in early postpartum dairy cows experimentally challenged with either endotoxin ( n = 6) or E. coli ( n = 6). The AOAH activity of blood neutrophils started to decrease significantly at post challenge hours ( PCH) 6 - 24 and 12 - 24 in the endotoxin and E. coli-challenged groups, respectively; it returned to pre-challenged values at PCH 48 in both endotoxin- and E. coli-challenged groups. The cows were classified as moderate and severe responders according to milk production loss in the non-challenged quarters at PCH 48. There were no severe responders in the endotoxin- challenged group. In the E. coli-challenged group, only 1 severe responder was identified. The pre-challenge neutrophil AOAH activity of the severe responder was similar to 30% lower than that of moderate responders. No LPS was detected in the plasma of endotoxin- challenged cows; neither was it found in the plasma of moderate responders in the E. coli-challenged group at any PCH. However, at PCH 6, a remarkable amount of LPS was detected in the plasma of the severe responder from the E. coli-challenged group. Furthermore, neutrophil AOAH activity was increased by similar to 70% in the severe responder at PCH 6, but it increased by only similar to 15% in moderate responders. This was followed by a decreased neutrophil AOAH activity at PCH 12 - 24 and 24 - 72 in moderate and severe responders, respectively; the decreased AOAH activity at those PCH was more pronounced in the severe responder. The pronounced decreased neutrophil AOAH activity during mastitis often coincided with extreme leukopenia, neutropenia and a maximal number of immature neutrophils in the blood. Our results demonstrate that a decrease in neutrophil AOAH activity results in the appearance of LPS in the blood, and low blood neutrophil deacylation activity could be considered as a risk factor for severe clinical coliform mastitis.
Keywords
endotoxin, acyloxyacyl hydrolase (AOAH), blood, mastitis, ESCHERICHIA-COLI, neutrophils, ENZYMATIC DEACYLATION, DAIRY-COWS, POLYMORPHONUCLEAR LEUKOCYTES, SEVERITY, LIPOPOLYSACCHARIDES, IN-VITRO, MILK NEUTROPHILS, PROTEINS, EXPRESSION

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MLA
MEHRZAD, J., et al. “Bovine Blood Neutrophil Acyloxyacyl Hydrolase (AOAH) Activity during Endotoxin and Coliform Mastitis.” VETERINARY RESEARCH, vol. 38, no. 5, EDP SCIENCES S A, 2007, pp. 655–68, doi:10.1051/vetres:2007024.
APA
MEHRZAD, J., DOSOGNE, H., De Spiegeleer, B., Duchateau, L., & Burvenich, C. (2007). Bovine blood neutrophil acyloxyacyl hydrolase (AOAH) activity during endotoxin and coliform mastitis. VETERINARY RESEARCH, 38(5), 655–668. https://doi.org/10.1051/vetres:2007024
Chicago author-date
MEHRZAD, J, H DOSOGNE, Bart De Spiegeleer, Luc Duchateau, and Christian Burvenich. 2007. “Bovine Blood Neutrophil Acyloxyacyl Hydrolase (AOAH) Activity during Endotoxin and Coliform Mastitis.” VETERINARY RESEARCH 38 (5): 655–68. https://doi.org/10.1051/vetres:2007024.
Chicago author-date (all authors)
MEHRZAD, J, H DOSOGNE, Bart De Spiegeleer, Luc Duchateau, and Christian Burvenich. 2007. “Bovine Blood Neutrophil Acyloxyacyl Hydrolase (AOAH) Activity during Endotoxin and Coliform Mastitis.” VETERINARY RESEARCH 38 (5): 655–668. doi:10.1051/vetres:2007024.
Vancouver
1.
MEHRZAD J, DOSOGNE H, De Spiegeleer B, Duchateau L, Burvenich C. Bovine blood neutrophil acyloxyacyl hydrolase (AOAH) activity during endotoxin and coliform mastitis. VETERINARY RESEARCH. 2007;38(5):655–68.
IEEE
[1]
J. MEHRZAD, H. DOSOGNE, B. De Spiegeleer, L. Duchateau, and C. Burvenich, “Bovine blood neutrophil acyloxyacyl hydrolase (AOAH) activity during endotoxin and coliform mastitis,” VETERINARY RESEARCH, vol. 38, no. 5, pp. 655–668, 2007.
@article{382558,
  abstract     = {{The dynamics of blood neutrophil acyloxyacyl hydrolase ( AOAH) activity, the appearance of endotoxin ( lipopolysaccharide, LPS) in blood and the role of blood neutrophil AOAH in the severity of Escherichia coli and endotoxin mastitis were investigated in early postpartum dairy cows experimentally challenged with either endotoxin ( n = 6) or E. coli ( n = 6). The AOAH activity of blood neutrophils started to decrease significantly at post challenge hours ( PCH) 6 - 24 and 12 - 24 in the endotoxin and E. coli-challenged groups, respectively; it returned to pre-challenged values at PCH 48 in both endotoxin- and E. coli-challenged groups. The cows were classified as moderate and severe responders according to milk production loss in the non-challenged quarters at PCH 48. There were no severe responders in the endotoxin- challenged group. In the E. coli-challenged group, only 1 severe responder was identified. The pre-challenge neutrophil AOAH activity of the severe responder was similar to 30% lower than that of moderate responders. No LPS was detected in the plasma of endotoxin- challenged cows; neither was it found in the plasma of moderate responders in the E. coli-challenged group at any PCH. However, at PCH 6, a remarkable amount of LPS was detected in the plasma of the severe responder from the E. coli-challenged group. Furthermore, neutrophil AOAH activity was increased by similar to 70% in the severe responder at PCH 6, but it increased by only similar to 15% in moderate responders. This was followed by a decreased neutrophil AOAH activity at PCH 12 - 24 and 24 - 72 in moderate and severe responders, respectively; the decreased AOAH activity at those PCH was more pronounced in the severe responder. The pronounced decreased neutrophil AOAH activity during mastitis often coincided with extreme leukopenia, neutropenia and a maximal number of immature neutrophils in the blood. Our results demonstrate that a decrease in neutrophil AOAH activity results in the appearance of LPS in the blood, and low blood neutrophil deacylation activity could be considered as a risk factor for severe clinical coliform mastitis.}},
  author       = {{MEHRZAD, J and DOSOGNE, H and De Spiegeleer, Bart and Duchateau, Luc and Burvenich, Christian}},
  issn         = {{0928-4249}},
  journal      = {{VETERINARY RESEARCH}},
  keywords     = {{endotoxin,acyloxyacyl hydrolase (AOAH),blood,mastitis,ESCHERICHIA-COLI,neutrophils,ENZYMATIC DEACYLATION,DAIRY-COWS,POLYMORPHONUCLEAR LEUKOCYTES,SEVERITY,LIPOPOLYSACCHARIDES,IN-VITRO,MILK NEUTROPHILS,PROTEINS,EXPRESSION}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{655--668}},
  publisher    = {{EDP SCIENCES S A}},
  title        = {{Bovine blood neutrophil acyloxyacyl hydrolase (AOAH) activity during endotoxin and coliform mastitis}},
  url          = {{http://doi.org/10.1051/vetres:2007024}},
  volume       = {{38}},
  year         = {{2007}},
}

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