Effect on quarter milk somatic cell count and antimicrobial susceptibility of Staphylococcus rostri causing intramammary infection in dairy water buffaloes
- Author
- C Locatelli, Sofie Piepers (UGent) , Sarne De Vliegher (UGent) , A Barberio, Karlien Supré, L Scaccabarozzi, G Pisoni, V Bronzo, Freddy Haesebrouck (UGent) and P Moroni
- Organization
- Abstract
- In many parts of the world, coagulase-negative staphylococci (CNS) are the predominant cause of intramammary infections (IMI) in dairy cows and in water buffaloes, as well. A longitudinal field study was carried out on one well-managed dairy water buffalo herd to determine the prevalence and distribution of CNS and a recently described CNS-species, Staphylococcus rostri, in milk samples to explore its relevance for buffaloes' udder health throughout lactation, and to gain insight into the susceptibility of the latter species toward commonly used antimicrobials. Twice weekly quarter milk samples from a cohort of 11 lactating water buffaloes were collected over an 8-mo period. The CNS (n = 109; 76.2% of all culture-positive samples) were the predominant pathogens causing IMI, followed by Corynebacterium bovis (n = 11; 7.6%) and Streptococcus spp. (n = 9; 6.2%) other than Stretococcus uberis (n = 2; 1.4%). Thirty-seven hemolytic staphylococci suspected to be Staphylococcus aureus were further differentiated using transfer DNA-intergenic spacer-PCR and rpoB-gene sequencing because they were coagulase-negative. Thirty-three of those isolates were identified as Staph. rostri, whereas 2 others were identified as Staphylococcus epidermidis. None of the Staph. rostri isolates displayed resistance to the antimicrobial agents tested. Mean quarter milk somatic cell count (qSCC) of all samples collected throughout lactation was 20,970 cells/mL. The qSCC at sampling of quarters infected with Staph. rostri (34,466 cells/mL) and CNS other than Staph. rostri (34,813 cells/mL) were significantly higher than the qSCC of noninfected quarters (20,287 cells/mL), yet not significantly different from each other. These findings provide novel insight into the prevalence and distribution, antimicrobial susceptibility, and relevance of Staph. rostri compared with other CNS species causing IMI in water buffaloes. Further studies are needed to pinpoint the relevance, niches, and transmission routes of Staph. rostri, as well as other CNS in water buffaloes.
- Keywords
- RESISTANCE, MASTITIS, COWS, PREVALENCE, IDENTIFICATION, DIVERSITY, SAMPLES, Staphylococcus rostri, water buffalo, HEALTHY PIGS, antimicrobial susceptibility, intramammary infection
Downloads
-
(...).pdf
- full text
- |
- UGent only
- |
- |
- 197.38 KB
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-3823448
- MLA
- Locatelli, C., et al. “Effect on Quarter Milk Somatic Cell Count and Antimicrobial Susceptibility of Staphylococcus Rostri Causing Intramammary Infection in Dairy Water Buffaloes.” JOURNAL OF DAIRY SCIENCE, vol. 96, no. 6, 2013, pp. 3799–805, doi:10.3168/jds.2012-6275.
- APA
- Locatelli, C., Piepers, S., De Vliegher, S., Barberio, A., Supré, K., Scaccabarozzi, L., … Moroni, P. (2013). Effect on quarter milk somatic cell count and antimicrobial susceptibility of Staphylococcus rostri causing intramammary infection in dairy water buffaloes. JOURNAL OF DAIRY SCIENCE, 96(6), 3799–3805. https://doi.org/10.3168/jds.2012-6275
- Chicago author-date
- Locatelli, C, Sofie Piepers, Sarne De Vliegher, A Barberio, Karlien Supré, L Scaccabarozzi, G Pisoni, V Bronzo, Freddy Haesebrouck, and P Moroni. 2013. “Effect on Quarter Milk Somatic Cell Count and Antimicrobial Susceptibility of Staphylococcus Rostri Causing Intramammary Infection in Dairy Water Buffaloes.” JOURNAL OF DAIRY SCIENCE 96 (6): 3799–3805. https://doi.org/10.3168/jds.2012-6275.
- Chicago author-date (all authors)
- Locatelli, C, Sofie Piepers, Sarne De Vliegher, A Barberio, Karlien Supré, L Scaccabarozzi, G Pisoni, V Bronzo, Freddy Haesebrouck, and P Moroni. 2013. “Effect on Quarter Milk Somatic Cell Count and Antimicrobial Susceptibility of Staphylococcus Rostri Causing Intramammary Infection in Dairy Water Buffaloes.” JOURNAL OF DAIRY SCIENCE 96 (6): 3799–3805. doi:10.3168/jds.2012-6275.
- Vancouver
- 1.Locatelli C, Piepers S, De Vliegher S, Barberio A, Supré K, Scaccabarozzi L, et al. Effect on quarter milk somatic cell count and antimicrobial susceptibility of Staphylococcus rostri causing intramammary infection in dairy water buffaloes. JOURNAL OF DAIRY SCIENCE. 2013;96(6):3799–805.
- IEEE
- [1]C. Locatelli et al., “Effect on quarter milk somatic cell count and antimicrobial susceptibility of Staphylococcus rostri causing intramammary infection in dairy water buffaloes,” JOURNAL OF DAIRY SCIENCE, vol. 96, no. 6, pp. 3799–3805, 2013.
@article{3823448, abstract = {{In many parts of the world, coagulase-negative staphylococci (CNS) are the predominant cause of intramammary infections (IMI) in dairy cows and in water buffaloes, as well. A longitudinal field study was carried out on one well-managed dairy water buffalo herd to determine the prevalence and distribution of CNS and a recently described CNS-species, Staphylococcus rostri, in milk samples to explore its relevance for buffaloes' udder health throughout lactation, and to gain insight into the susceptibility of the latter species toward commonly used antimicrobials. Twice weekly quarter milk samples from a cohort of 11 lactating water buffaloes were collected over an 8-mo period. The CNS (n = 109; 76.2% of all culture-positive samples) were the predominant pathogens causing IMI, followed by Corynebacterium bovis (n = 11; 7.6%) and Streptococcus spp. (n = 9; 6.2%) other than Stretococcus uberis (n = 2; 1.4%). Thirty-seven hemolytic staphylococci suspected to be Staphylococcus aureus were further differentiated using transfer DNA-intergenic spacer-PCR and rpoB-gene sequencing because they were coagulase-negative. Thirty-three of those isolates were identified as Staph. rostri, whereas 2 others were identified as Staphylococcus epidermidis. None of the Staph. rostri isolates displayed resistance to the antimicrobial agents tested. Mean quarter milk somatic cell count (qSCC) of all samples collected throughout lactation was 20,970 cells/mL. The qSCC at sampling of quarters infected with Staph. rostri (34,466 cells/mL) and CNS other than Staph. rostri (34,813 cells/mL) were significantly higher than the qSCC of noninfected quarters (20,287 cells/mL), yet not significantly different from each other. These findings provide novel insight into the prevalence and distribution, antimicrobial susceptibility, and relevance of Staph. rostri compared with other CNS species causing IMI in water buffaloes. Further studies are needed to pinpoint the relevance, niches, and transmission routes of Staph. rostri, as well as other CNS in water buffaloes.}}, author = {{Locatelli, C and Piepers, Sofie and De Vliegher, Sarne and Barberio, A and Supré, Karlien and Scaccabarozzi, L and Pisoni, G and Bronzo, V and Haesebrouck, Freddy and Moroni, P}}, issn = {{0022-0302}}, journal = {{JOURNAL OF DAIRY SCIENCE}}, keywords = {{RESISTANCE,MASTITIS,COWS,PREVALENCE,IDENTIFICATION,DIVERSITY,SAMPLES,Staphylococcus rostri,water buffalo,HEALTHY PIGS,antimicrobial susceptibility,intramammary infection}}, language = {{eng}}, number = {{6}}, pages = {{3799--3805}}, title = {{Effect on quarter milk somatic cell count and antimicrobial susceptibility of Staphylococcus rostri causing intramammary infection in dairy water buffaloes}}, url = {{http://doi.org/10.3168/jds.2012-6275}}, volume = {{96}}, year = {{2013}}, }
- Altmetric
- View in Altmetric
- Web of Science
- Times cited: