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Poly-ß-hydroxybutyrate-accumulating bacteria protect gnotobiotic Artemia franciscana from pathogenic Vibrio campbellii

Dirk Halet, Tom Defoirdt (UGent) , Petra Van Damme (UGent) , Han Vervaeren (UGent) , Ilse Forrez (UGent) , Tom Van de Wiele (UGent) , Nico Boon (UGent) , Patrick Sorgeloos (UGent) , Peter Bossier (UGent) and Willy Verstraete (UGent)
(2007) FEMS MICROBIOLOGY ECOLOGY. 60(3). p.363-369
Author
Organization
Abstract
A poly-beta-hydroxybutyrate (PHB)-accumulating enrichment culture was obtained using activated sludge from a polyphosphate-accumulating reactor as inoculum. PHB accumulated by the enrichment culture significantly enhanced the survival of Artemia nauplii, infected with the virulent pathogen Vibrio campbellii LMG 21363. A strain was isolated from the enrichment culture, based on its ability to accumulate PHB, and 16S rRNA gene sequencing of the isolate revealed 99% sequence similarity to Brachymonas denitrificans AS-P1. The isolate, named PHB2, showed good PHB-accumulating activity (up to 32% of the cell dry weight). PHB accumulated by isolate PHB2 was able to protect Artemia completely from the V. campbellii strain. Our data indicate that PHB-accumulating bacteria, such as B. denitrificans PHB2, could be used as an an effective and economically interesting alternative strategy to control infections in aquaculture.
Keywords
REMOVAL, PHB, HARVEYI, ESCHERICHIA-COLI, SLUDGE, POLYHYDROXYALKANOATES, AEROMONAS-HYDROPHILA, ANTIBIOTIC-RESISTANCE, COMAMONAS-TESTOSTERONI, Brachymonas denitrificans, BRACHYMONAS-DENITRIFICANS, luminescent vibriosis, brine shrimp, ACIDS

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MLA
Halet, Dirk, et al. “Poly-ß-Hydroxybutyrate-Accumulating Bacteria Protect Gnotobiotic Artemia Franciscana from Pathogenic Vibrio Campbellii.” FEMS MICROBIOLOGY ECOLOGY, vol. 60, no. 3, 2007, pp. 363–69, doi:10.1111/j.1574-6941.2007.00305.x.
APA
Halet, D., Defoirdt, T., Van Damme, P., Vervaeren, H., Forrez, I., Van de Wiele, T., … Verstraete, W. (2007). Poly-ß-hydroxybutyrate-accumulating bacteria protect gnotobiotic Artemia franciscana from pathogenic Vibrio campbellii. FEMS MICROBIOLOGY ECOLOGY, 60(3), 363–369. https://doi.org/10.1111/j.1574-6941.2007.00305.x
Chicago author-date
Halet, Dirk, Tom Defoirdt, Petra Van Damme, Han Vervaeren, Ilse Forrez, Tom Van de Wiele, Nico Boon, Patrick Sorgeloos, Peter Bossier, and Willy Verstraete. 2007. “Poly-ß-Hydroxybutyrate-Accumulating Bacteria Protect Gnotobiotic Artemia Franciscana from Pathogenic Vibrio Campbellii.” FEMS MICROBIOLOGY ECOLOGY 60 (3): 363–69. https://doi.org/10.1111/j.1574-6941.2007.00305.x.
Chicago author-date (all authors)
Halet, Dirk, Tom Defoirdt, Petra Van Damme, Han Vervaeren, Ilse Forrez, Tom Van de Wiele, Nico Boon, Patrick Sorgeloos, Peter Bossier, and Willy Verstraete. 2007. “Poly-ß-Hydroxybutyrate-Accumulating Bacteria Protect Gnotobiotic Artemia Franciscana from Pathogenic Vibrio Campbellii.” FEMS MICROBIOLOGY ECOLOGY 60 (3): 363–369. doi:10.1111/j.1574-6941.2007.00305.x.
Vancouver
1.
Halet D, Defoirdt T, Van Damme P, Vervaeren H, Forrez I, Van de Wiele T, et al. Poly-ß-hydroxybutyrate-accumulating bacteria protect gnotobiotic Artemia franciscana from pathogenic Vibrio campbellii. FEMS MICROBIOLOGY ECOLOGY. 2007;60(3):363–9.
IEEE
[1]
D. Halet et al., “Poly-ß-hydroxybutyrate-accumulating bacteria protect gnotobiotic Artemia franciscana from pathogenic Vibrio campbellii,” FEMS MICROBIOLOGY ECOLOGY, vol. 60, no. 3, pp. 363–369, 2007.
@article{381929,
  abstract     = {{A poly-beta-hydroxybutyrate (PHB)-accumulating enrichment culture was obtained using activated sludge from a polyphosphate-accumulating reactor as inoculum. PHB accumulated by the enrichment culture significantly enhanced the survival of Artemia nauplii, infected with the virulent pathogen Vibrio campbellii LMG 21363. A strain was isolated from the enrichment culture, based on its ability to accumulate PHB, and 16S rRNA gene sequencing of the isolate revealed 99% sequence similarity to Brachymonas denitrificans AS-P1. The isolate, named PHB2, showed good PHB-accumulating activity (up to 32% of the cell dry weight). PHB accumulated by isolate PHB2 was able to protect Artemia completely from the V. campbellii strain. Our data indicate that PHB-accumulating bacteria, such as B. denitrificans PHB2, could be used as an an effective and economically interesting alternative strategy to control infections in aquaculture.}},
  author       = {{Halet, Dirk and Defoirdt, Tom and Van Damme, Petra and Vervaeren, Han and Forrez, Ilse and Van de Wiele, Tom and Boon, Nico and Sorgeloos, Patrick and Bossier, Peter and Verstraete, Willy}},
  issn         = {{0168-6496}},
  journal      = {{FEMS MICROBIOLOGY ECOLOGY}},
  keywords     = {{REMOVAL,PHB,HARVEYI,ESCHERICHIA-COLI,SLUDGE,POLYHYDROXYALKANOATES,AEROMONAS-HYDROPHILA,ANTIBIOTIC-RESISTANCE,COMAMONAS-TESTOSTERONI,Brachymonas denitrificans,BRACHYMONAS-DENITRIFICANS,luminescent vibriosis,brine shrimp,ACIDS}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{363--369}},
  title        = {{Poly-ß-hydroxybutyrate-accumulating bacteria protect gnotobiotic Artemia franciscana from pathogenic Vibrio campbellii}},
  url          = {{http://dx.doi.org/10.1111/j.1574-6941.2007.00305.x}},
  volume       = {{60}},
  year         = {{2007}},
}

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