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Effect of ovotransferrin and lactoferrins on Chlamydophila psittaci adhesion and invasion in HD11 chicken macrophages

Delphine Sylvie Anne Beeckman UGent, Caroline Van Droogenbroeck UGent, Bart De Cock UGent, Patric Van Oostveldt UGent and Daisy Vanrompay UGent (2007) VETERINARY RESEARCH. 38(5). p.729-739
abstract
The effect of ovotransferrin ( ovoTF), human lactoferrin ( hLF) and bovine lactoferrin ( bLF) on the obligate intracellular pathogen Chlamydophila ( Cp.) psittaci was evaluated using a model of Buffalo Green Monkey kidney ( BGM) cells and HD11 chicken macrophages as artificial hosts. Firstly, the effect of transferrins on the infectivity of the bacteria was evaluated. Pre-incubation of Cp. psittaci with 0.5 to 5 mg/mL ovoTF prior to infecting BGM cells significantly lowered the infection rate ( P < 0.05). For both lactoferrins, the infection rate could only be reduced with 5 mg/mL, albeit not significantly as compared to the infection rate created by the untreated bacteria. Secondly, transferrins were tested for their ability to influence bacterial adhesion and entry in HD11 cells. Maximal non-cytotoxic and non-bactericidal concentrations of 0.05 mg/mL ovoTF and 0.5 mg/mL hLF and bLF were used. Overall, ovoTF was more effective than human and bovine LF in inhibiting bacterial irreversible attachment and cell entry and the latter was accompanied by a dose-dependent reduction of actin recruitment at the bacterial entry site. However, once bacteria had entered HD11 cells, transferrins had apparently no effect on intracellular replication. The present findings suggest a possible role for transferrins and especially ovoTF, in preventing avian Cp. psittaci infections.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Chlamydophila psittaci, Chlamydia, ovotransferrin, lactoferrin, OUTER-MEMBRANE PROTEIN, AVIAN CHLAMYDIOSIS, ESCHERICHIA-COLI, EPITHELIAL-CELLS, HEPARAN-SULFATE, BINDING, BACTERIAL, DAMAGE, ACTIN, ENTRY
journal title
VETERINARY RESEARCH
Vet. Res.
volume
38
issue
5
pages
729 - 739
Web of Science type
Article
Web of Science id
000249779400006
JCR category
VETERINARY SCIENCES
JCR impact factor
4.125 (2007)
JCR rank
1/122 (2007)
JCR quartile
1 (2007)
ISSN
0928-4249
DOI
10.1051/vetres:2007028
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
380665
handle
http://hdl.handle.net/1854/LU-380665
date created
2007-10-26 08:59:00
date last changed
2015-06-17 10:45:31
@article{380665,
  abstract     = {The effect of ovotransferrin ( ovoTF), human lactoferrin ( hLF) and bovine lactoferrin ( bLF) on the obligate intracellular pathogen Chlamydophila ( Cp.) psittaci was evaluated using a model of Buffalo Green Monkey kidney ( BGM) cells and HD11 chicken macrophages as artificial hosts. Firstly, the effect of transferrins on the infectivity of the bacteria was evaluated. Pre-incubation of Cp. psittaci with 0.5 to 5 mg/mL ovoTF prior to infecting BGM cells significantly lowered the infection rate ( P {\textlangle} 0.05). For both lactoferrins, the infection rate could only be reduced with 5 mg/mL, albeit not significantly as compared to the infection rate created by the untreated bacteria. Secondly, transferrins were tested for their ability to influence bacterial adhesion and entry in HD11 cells. Maximal non-cytotoxic and non-bactericidal concentrations of 0.05 mg/mL ovoTF and 0.5 mg/mL hLF and bLF were used. Overall, ovoTF was more effective than human and bovine LF in inhibiting bacterial irreversible attachment and cell entry and the latter was accompanied by a dose-dependent reduction of actin recruitment at the bacterial entry site. However, once bacteria had entered HD11 cells, transferrins had apparently no effect on intracellular replication. The present findings suggest a possible role for transferrins and especially ovoTF, in preventing avian Cp. psittaci infections.},
  author       = {Beeckman, Delphine Sylvie Anne and Van Droogenbroeck, Caroline and De Cock, Bart and Van Oostveldt, Patric and Vanrompay, Daisy},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keyword      = {Chlamydophila psittaci,Chlamydia,ovotransferrin,lactoferrin,OUTER-MEMBRANE PROTEIN,AVIAN CHLAMYDIOSIS,ESCHERICHIA-COLI,EPITHELIAL-CELLS,HEPARAN-SULFATE,BINDING,BACTERIAL,DAMAGE,ACTIN,ENTRY},
  language     = {eng},
  number       = {5},
  pages        = {729--739},
  title        = {Effect of ovotransferrin and lactoferrins on Chlamydophila psittaci adhesion and invasion in HD11 chicken macrophages},
  url          = {http://dx.doi.org/10.1051/vetres:2007028},
  volume       = {38},
  year         = {2007},
}

Chicago
Beeckman, Delphine Sylvie Anne, Caroline Van Droogenbroeck, Bart De Cock, Patric Van Oostveldt, and Daisy Vanrompay. 2007. “Effect of Ovotransferrin and Lactoferrins on Chlamydophila Psittaci Adhesion and Invasion in HD11 Chicken Macrophages.” Veterinary Research 38 (5): 729–739.
APA
Beeckman, D. S. A., Van Droogenbroeck, C., De Cock, B., Van Oostveldt, P., & Vanrompay, D. (2007). Effect of ovotransferrin and lactoferrins on Chlamydophila psittaci adhesion and invasion in HD11 chicken macrophages. VETERINARY RESEARCH, 38(5), 729–739.
Vancouver
1.
Beeckman DSA, Van Droogenbroeck C, De Cock B, Van Oostveldt P, Vanrompay D. Effect of ovotransferrin and lactoferrins on Chlamydophila psittaci adhesion and invasion in HD11 chicken macrophages. VETERINARY RESEARCH. 2007;38(5):729–39.
MLA
Beeckman, Delphine Sylvie Anne, Caroline Van Droogenbroeck, Bart De Cock, et al. “Effect of Ovotransferrin and Lactoferrins on Chlamydophila Psittaci Adhesion and Invasion in HD11 Chicken Macrophages.” VETERINARY RESEARCH 38.5 (2007): 729–739. Print.