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Resistance to Botrytis cinerea in sitiens, an abscisic acid-deficient tomato mutant, involves timely production of hydrogen peroxide and cell wall modifications in the epidermis

Bob Asselbergh (UGent) , Katrien Curvers (UGent) , Soraya de Carvalho França (UGent) , Kris Audenaert (UGent) , Marnik Vuylsteke (UGent) , Frank Van Breusegem (UGent) and Monica Höfte (UGent)
(2007) PLANT PHYSIOLOGY. 144(4). p.1863-1877
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Abstract
Plant defense mechanisms against necrotrophic pathogens, such as Botrytis cinerea, are considered to be complex and to differ from those that are effective against biotrophs. In the abscisic acid-deficient sitiens tomato (Solanum lycopersicum) mutant, which is highly resistant to B. cinerea, accumulation of hydrogen peroxide (H2O2) was earlier and stronger than in the susceptible wild type at the site of infection. In sitiens, H2O2 accumulation was observed from 4 h postinoculation (hpi), specifically in the leaf epidermal cell walls, where it caused modification by protein cross-linking and incorporation of phenolic compounds. In wildtype tomato plants, H2O2 started to accumulate 24 hpi in the mesophyll layer and was associated with spreading cell death. Transcript-profiling analysis using TOM1 microarrays revealed that defense-related transcript accumulation prior to infection was higher in sitiens than in wild type. Moreover, further elevation of sitiens defense gene expression was stronger than in wild type 8 hpi both in number of genes and in their expression levels and confirmed a role for cell wall modification in the resistant reaction. Although, in general, plant defense-related reactive oxygen species formation facilitates necrotrophic colonization, these data indicate that timely hyperinduction of H2O2-dependent defenses in the epidermal cell wall can effectively block early development of B. cinerea.
Keywords
HYPERSENSITIVE RESPONSE, OXIDATIVE CROSS-LINKING, DISEASE RESISTANCE, SALICYLIC-ACID, NECROTROPHIC PATHOGEN, DEFENSE GENE-EXPRESSION, PLANT-PATHOGEN INTERACTIONS, PHYTOPHTHORA-INFESTANS, OIDIUM-NEOLYCOPERSICI, ARABIDOPSIS-THALIANA

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Chicago
Asselbergh, Bob, Katrien Curvers, Soraya de Carvalho França, Kris Audenaert, Marnik Vuylsteke, Frank Van Breusegem, and Monica Höfte. 2007. “Resistance to Botrytis Cinerea in Sitiens, an Abscisic Acid-deficient Tomato Mutant, Involves Timely Production of Hydrogen Peroxide and Cell Wall Modifications in the Epidermis.” Plant Physiology 144 (4): 1863–1877.
APA
Asselbergh, Bob, Curvers, K., de Carvalho França, S., Audenaert, K., Vuylsteke, M., Van Breusegem, F., & Höfte, M. (2007). Resistance to Botrytis cinerea in sitiens, an abscisic acid-deficient tomato mutant, involves timely production of hydrogen peroxide and cell wall modifications in the epidermis. PLANT PHYSIOLOGY, 144(4), 1863–1877.
Vancouver
1.
Asselbergh B, Curvers K, de Carvalho França S, Audenaert K, Vuylsteke M, Van Breusegem F, et al. Resistance to Botrytis cinerea in sitiens, an abscisic acid-deficient tomato mutant, involves timely production of hydrogen peroxide and cell wall modifications in the epidermis. PLANT PHYSIOLOGY. 2007;144(4):1863–77.
MLA
Asselbergh, Bob, Katrien Curvers, Soraya de Carvalho França, et al. “Resistance to Botrytis Cinerea in Sitiens, an Abscisic Acid-deficient Tomato Mutant, Involves Timely Production of Hydrogen Peroxide and Cell Wall Modifications in the Epidermis.” PLANT PHYSIOLOGY 144.4 (2007): 1863–1877. Print.
@article{379945,
  abstract     = {Plant defense mechanisms against necrotrophic pathogens, such as Botrytis cinerea, are considered to be complex and to differ from those that are effective against biotrophs. In the abscisic acid-deficient sitiens tomato (Solanum lycopersicum) mutant, which is highly resistant to B. cinerea, accumulation of hydrogen peroxide (H2O2) was earlier and stronger than in the susceptible wild type at the site of infection. In sitiens, H2O2 accumulation was observed from 4 h postinoculation (hpi), specifically in the leaf epidermal cell walls, where it caused modification by protein cross-linking and incorporation of phenolic compounds. In wildtype tomato plants, H2O2 started to accumulate 24 hpi in the mesophyll layer and was associated with spreading cell death. Transcript-profiling analysis using TOM1 microarrays revealed that defense-related transcript accumulation prior to infection was higher in sitiens than in wild type. Moreover, further elevation of sitiens defense gene expression was stronger than in wild type 8 hpi both in number of genes and in their expression levels and confirmed a role for cell wall modification in the resistant reaction. Although, in general, plant defense-related reactive oxygen species formation facilitates necrotrophic colonization, these data indicate that timely hyperinduction of H2O2-dependent defenses in the epidermal cell wall can effectively block early development of B. cinerea.},
  author       = {Asselbergh, Bob and Curvers, Katrien and de Carvalho Fran\c{c}a, Soraya and Audenaert, Kris and Vuylsteke, Marnik and Van Breusegem, Frank and H{\"o}fte, Monica},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {HYPERSENSITIVE RESPONSE,OXIDATIVE CROSS-LINKING,DISEASE RESISTANCE,SALICYLIC-ACID,NECROTROPHIC PATHOGEN,DEFENSE GENE-EXPRESSION,PLANT-PATHOGEN INTERACTIONS,PHYTOPHTHORA-INFESTANS,OIDIUM-NEOLYCOPERSICI,ARABIDOPSIS-THALIANA},
  language     = {eng},
  number       = {4},
  pages        = {1863--1877},
  title        = {Resistance to Botrytis cinerea in sitiens, an abscisic acid-deficient tomato mutant, involves timely production of hydrogen peroxide and cell wall modifications in the epidermis},
  url          = {http://dx.doi.org/10.1104/pp.107.099226},
  volume       = {144},
  year         = {2007},
}

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