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Accumulation of dendritic cells and increased CCL20 levels in the airways of patients with chronic obstructive pulmonary disease

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Abstract
Rationale: Chronic obstructive pulmonary disease (COPD) is characterized by chronic airway inflammation. It is unclear if dendritic cells (DC) participate in this inflammatory process. Objectives: To evaluate the presence of DC in small airways of patients with COPD. Methods: We evaluated DC infiltration in small airways by immunohistochemistry in patients with COPD (stage I-IV), never-smokers, and smokers without COPD. Chemokine ligand 20 (CCL20, the most potent chemokine in attracting DC) was determined in total lung by RT-PCR and in induced sputum by enzyme-linked immunsorbent assay. Chemokine receptor 6 (CCR6, the receptor for CCL20) expression on human pulmonary DC was evaluated by RT-PCR and flow cytometry. Measurements and Main Results: There is a significant increase in DC number in the epithelium (p = 0.007) and adventitia (p = 0.009) of small airways of patients with COPD compared with never-smokers and smokers without COPD. DC number in epithelium and adventitia increases along with disease severity. CCL20 mRNA expression in total lung and CCL20 protein levels in induced sputum are significantly higher in patients with COPD compared with never-smokers (p = 0.034 for CCL20 mRNA and p = 0.0008 for CCL20 protein) and smokers without COPD (p = 0.016 for CCL20 mRNA and p = 0.001 for CCL20 protein). DC isolated from human lung express CCR6 both at mRNA and at protein level. Conclusions: This is the first description of airway infiltration by DC in COPD. Moreover, interaction between CCL20 and CCR6 provides a possible mechanism for accumulation of DC in the lungs in COPD.
Keywords
SUBSETS, langerin, EMPHYSEMA, HUMAN LUNG, COPD, MICE, CIGARETTE-SMOKING, LANGERHANS CELLS, MACROPHAGE ELASTASE, CCL20, CCR6, INFLAMMATORY PROTEIN 3-ALPHA, CHEMOKINE RECEPTOR EXPRESSION

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Citation

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Chicago
Demedts, Ingel, Ken Bracke, Geert Van Pottelberge, Dries Testelmans, Geert M Verleden, Frank Vermassen, Guy Joos, and Guy Brusselle. 2007. “Accumulation of Dendritic Cells and Increased CCL20 Levels in the Airways of Patients with Chronic Obstructive Pulmonary Disease.” American Journal of Respiratory and Critical Care Medicine 175 (10): 998–1005.
APA
Demedts, I., Bracke, K., Van Pottelberge, G., Testelmans, D., Verleden, G. M., Vermassen, F., Joos, G., et al. (2007). Accumulation of dendritic cells and increased CCL20 levels in the airways of patients with chronic obstructive pulmonary disease. AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 175(10), 998–1005.
Vancouver
1.
Demedts I, Bracke K, Van Pottelberge G, Testelmans D, Verleden GM, Vermassen F, et al. Accumulation of dendritic cells and increased CCL20 levels in the airways of patients with chronic obstructive pulmonary disease. AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE. 2007;175(10):998–1005.
MLA
Demedts, Ingel, Ken Bracke, Geert Van Pottelberge, et al. “Accumulation of Dendritic Cells and Increased CCL20 Levels in the Airways of Patients with Chronic Obstructive Pulmonary Disease.” AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE 175.10 (2007): 998–1005. Print.
@article{378593,
  abstract     = {Rationale: Chronic obstructive pulmonary disease (COPD) is characterized by chronic airway inflammation. It is unclear if dendritic cells (DC) participate in this inflammatory process.
Objectives: To evaluate the presence of DC in small airways of patients with COPD.
Methods: We evaluated DC infiltration in small airways by immunohistochemistry in patients with COPD (stage I-IV), never-smokers, and smokers without COPD. Chemokine ligand 20 (CCL20, the most potent chemokine in attracting DC) was determined in total lung by RT-PCR and in induced sputum by enzyme-linked immunsorbent assay. Chemokine receptor 6 (CCR6, the receptor for CCL20) expression on human pulmonary DC was evaluated by RT-PCR and flow cytometry.
Measurements and Main Results: There is a significant increase in DC number in the epithelium (p = 0.007) and adventitia (p = 0.009) of small airways of patients with COPD compared with never-smokers and smokers without COPD. DC number in epithelium and adventitia increases along with disease severity. CCL20 mRNA expression in total lung and CCL20 protein levels in induced sputum are significantly higher in patients with COPD compared with never-smokers (p = 0.034 for CCL20 mRNA and p = 0.0008 for CCL20 protein) and smokers without COPD (p = 0.016 for CCL20 mRNA and p = 0.001 for CCL20 protein). DC isolated from human lung express CCR6 both at mRNA and at protein level.
Conclusions: This is the first description of airway infiltration by DC in COPD. Moreover, interaction between CCL20 and CCR6 provides a possible mechanism for accumulation of DC in the lungs in COPD.},
  author       = {Demedts, Ingel and Bracke, Ken and Van Pottelberge, Geert and Testelmans, Dries and Verleden, Geert M and Vermassen, Frank and Joos, Guy and Brusselle, Guy},
  issn         = {1073-449X},
  journal      = {AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE},
  keywords     = {SUBSETS,langerin,EMPHYSEMA,HUMAN LUNG,COPD,MICE,CIGARETTE-SMOKING,LANGERHANS CELLS,MACROPHAGE ELASTASE,CCL20,CCR6,INFLAMMATORY PROTEIN 3-ALPHA,CHEMOKINE RECEPTOR EXPRESSION},
  language     = {eng},
  number       = {10},
  pages        = {998--1005},
  title        = {Accumulation of dendritic cells and increased CCL20 levels in the airways of patients with chronic obstructive pulmonary disease},
  url          = {http://dx.doi.org/10.1164/rccm.200608-1113OC},
  volume       = {175},
  year         = {2007},
}

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