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Identification of lactobacilli isolated from the cloaca and vagina of laying hens and characterization for potential use as probiotics to control Salmonella Enteritidis

(2007) JOURNAL OF APPLIED MICROBIOLOGY. 102(4). p.1095-1106
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Abstract
Aims: To select Lactobacillus strains from laying hens for potential use as probiotic to control Salmonella Enteritidis infection. Methods and Results: One hundred and eighty-six lactobacilli were isolated from the cloaca and vagina of laying hens, and identified at the species level by a polyphasic taxonomic approach. All isolates belonged to the Lactobacillus acidophilus, Lactobacillus reuteri or Lactobacillus salivarius phylogenetic groups, with the L. reuteri group being the most predominant group. Based on genetic diversity, about 50 representative strains were selected and tested for in vitro properties that could be predictive for probiotic activity in laying hens. Salmonella inhibition was shown to be species dependent, and correlated to some extent with the production of lactic acid. A selection of strains was evaluated in a S. Enteritidis challenge experiment. Two strains, L. reuteri R-17485 and Lactobacillus johnsonii R-17504 significantly decreased the colonization of chicks by S. Enteritidis in caeca, liver and spleen. Conclusions: Lactobacilli isolated from laying hens were observed to inhibit Salmonella growth in vitro, most probably through production of lactic acid, and to decrease in vivo the S. Enteritidis colonization of chicks. Significance and Impact of the Study: The data demonstrate that Lactobacillus isolates from laying hens may have probiotic potential in reducing S. Enteritidis infection.
Keywords
Lactobacillus, identification, laying hens, probiotic, Salmonella, COMPETITIVE-EXCLUSION, IN-VITRO, SP-NOV., GASTROINTESTINAL-TRACT, BACTERIAL PATHOGENS, REPRODUCTIVE-TRACT, CHICKEN, COLONIZATION, INFECTION, POULTRY

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Citation

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MLA
Van Coillie, E, J Goris, Ilse Cleenwerck, et al. “Identification of Lactobacilli Isolated from the Cloaca and Vagina of Laying Hens and Characterization for Potential Use as Probiotics to Control Salmonella Enteritidis.” JOURNAL OF APPLIED MICROBIOLOGY 102.4 (2007): 1095–1106. Print.
APA
Van Coillie, E., Goris, J., Cleenwerck, I., Grijspeerdt, K., Botteldoorn, N., Van Immerseel, F., De Buck, J., et al. (2007). Identification of lactobacilli isolated from the cloaca and vagina of laying hens and characterization for potential use as probiotics to control Salmonella Enteritidis. JOURNAL OF APPLIED MICROBIOLOGY, 102(4), 1095–1106.
Chicago author-date
Van Coillie, E, J Goris, Ilse Cleenwerck, K Grijspeerdt, N Botteldoorn, Filip Van Immerseel, J De Buck, et al. 2007. “Identification of Lactobacilli Isolated from the Cloaca and Vagina of Laying Hens and Characterization for Potential Use as Probiotics to Control Salmonella Enteritidis.” Journal of Applied Microbiology 102 (4): 1095–1106.
Chicago author-date (all authors)
Van Coillie, E, J Goris, Ilse Cleenwerck, K Grijspeerdt, N Botteldoorn, Filip Van Immerseel, J De Buck, Marc Vancanneyt, Jean Swings, Lieve Herman, and Marc Heyndrickx. 2007. “Identification of Lactobacilli Isolated from the Cloaca and Vagina of Laying Hens and Characterization for Potential Use as Probiotics to Control Salmonella Enteritidis.” Journal of Applied Microbiology 102 (4): 1095–1106.
Vancouver
1.
Van Coillie E, Goris J, Cleenwerck I, Grijspeerdt K, Botteldoorn N, Van Immerseel F, et al. Identification of lactobacilli isolated from the cloaca and vagina of laying hens and characterization for potential use as probiotics to control Salmonella Enteritidis. JOURNAL OF APPLIED MICROBIOLOGY. 2007;102(4):1095–106.
IEEE
[1]
E. Van Coillie et al., “Identification of lactobacilli isolated from the cloaca and vagina of laying hens and characterization for potential use as probiotics to control Salmonella Enteritidis,” JOURNAL OF APPLIED MICROBIOLOGY, vol. 102, no. 4, pp. 1095–1106, 2007.
@article{376775,
  abstract     = {Aims: To select Lactobacillus strains from laying hens for potential use as probiotic to control Salmonella Enteritidis infection. 
Methods and Results: One hundred and eighty-six lactobacilli were isolated from the cloaca and vagina of laying hens, and identified at the species level by a polyphasic taxonomic approach. All isolates belonged to the Lactobacillus acidophilus, Lactobacillus reuteri or Lactobacillus salivarius phylogenetic groups, with the L. reuteri group being the most predominant group. Based on genetic diversity, about 50 representative strains were selected and tested for in vitro properties that could be predictive for probiotic activity in laying hens. Salmonella inhibition was shown to be species dependent, and correlated to some extent with the production of lactic acid. A selection of strains was evaluated in a S. Enteritidis challenge experiment. Two strains, L. reuteri R-17485 and Lactobacillus johnsonii R-17504 significantly decreased the colonization of chicks by S. Enteritidis in caeca, liver and spleen. 
Conclusions: Lactobacilli isolated from laying hens were observed to inhibit Salmonella growth in vitro, most probably through production of lactic acid, and to decrease in vivo the S. Enteritidis colonization of chicks. 
Significance and Impact of the Study: The data demonstrate that Lactobacillus isolates from laying hens may have probiotic potential in reducing S. Enteritidis infection.},
  author       = {Van Coillie, E and Goris, J and Cleenwerck, Ilse and Grijspeerdt, K and Botteldoorn, N and Van Immerseel, Filip and De Buck, J and Vancanneyt, Marc and Swings, Jean and Herman, Lieve and Heyndrickx, Marc},
  issn         = {1364-5072},
  journal      = {JOURNAL OF APPLIED MICROBIOLOGY},
  keywords     = {Lactobacillus,identification,laying hens,probiotic,Salmonella,COMPETITIVE-EXCLUSION,IN-VITRO,SP-NOV.,GASTROINTESTINAL-TRACT,BACTERIAL PATHOGENS,REPRODUCTIVE-TRACT,CHICKEN,COLONIZATION,INFECTION,POULTRY},
  language     = {eng},
  number       = {4},
  pages        = {1095--1106},
  title        = {Identification of lactobacilli isolated from the cloaca and vagina of laying hens and characterization for potential use as probiotics to control Salmonella Enteritidis},
  url          = {http://dx.doi.org/10.1111/j.1365-2672.2006.03164.x},
  volume       = {102},
  year         = {2007},
}

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