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An endoglucanase is involved in infection of rice roots by the not-cellulose-metabolizing endophyte Azoarcus sp. strain BH72

Barbara Reinhold-Hurek, Tamara Maes UGent, Sabrina Gemmer, Marc Van Montagu UGent and Thomas Hurek (2006) MOLECULAR PLANT-MICROBE INTERACTIONS. 19(2). p.181-188
abstract
The nitrogen-fixing endophyte Azoarcus sp. strain BH72 infects roots of Kallar grass and rice inter- and intra-cellularly and can spread systemically into shoots without causing symptoms of plant disease. Although cellulose or its breakdown products do not support growth, this strain expresses an endoglucanase, which might be involved in infection. Sequence analysis of eglA places the secreted 34-kDa protein into the glycosyl hydrolases family 5, with highest relatedness (40% identity) to endoglucanases of the phytopathogenic bacteria Xanthomonas campestris and Ralstonia solanacearum. Transcriptional regulation studied by eglA:: gusA fusion was not significantly affected by cellulose or its breakdown products or by microaerobiosis. Strongest induction (threefold) was obtained in bacteria grown in close vicinity to rice roots. Visible sites of expression were the emergence points of lateral roots and root tips, which are the primary regions of ingress into the root. To study the role in endophytic colonization, eglA was inactivated by transposon mutagenesis. Systemic spreading of the eglA mutant and of a pilAB mutant into the rice shoot could no longer be detected by polymerase chain reaction. Microscopic inspection of infection revealed that the intracellular colonization of root epidermis cells was significantly reduced in the eglA(-) mutant BHE6 compared with the wild type and partially restored in the complementation mutant BHRE2 expressing eglA. This provides evidence that Azoarcus sp. endoglucanase is an important determinant for successful endophytic colonization of rice roots, suggesting an active bacterial colonization process.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
RHIZOBIUM-MELILOTI, ESCHERICHIA-COLI, PSEUDOMONAS-SOLANACEARUM, ERWINIA-CAROTOVORA, CAROTOVORA SUBSP CAROTOVORA, BIOLOGICAL NITROGEN-FIXATION, transcription, pill, Oryza, cellulase, nitrogen fixation, KALLAR GRASS, SUGAR-CANE, NIF GENES, CLONING
journal title
MOLECULAR PLANT-MICROBE INTERACTIONS
Mol. Plant-Microbe Interact.
volume
19
issue
2
pages
181 - 188
Web of Science type
Article
Web of Science id
000234821700009
JCR category
PLANT SCIENCES
JCR impact factor
3.936 (2006)
JCR rank
10/145 (2006)
JCR quartile
1 (2006)
ISSN
0894-0282
DOI
10.1094/MPMI-19-0181
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
372289
handle
http://hdl.handle.net/1854/LU-372289
date created
2007-07-04 17:55:00
date last changed
2016-12-19 15:44:20
@article{372289,
  abstract     = {The nitrogen-fixing endophyte Azoarcus sp. strain BH72 infects roots of Kallar grass and rice inter- and intra-cellularly and can spread systemically into shoots without causing symptoms of plant disease. Although cellulose or its breakdown products do not support growth, this strain expresses an endoglucanase, which might be involved in infection. Sequence analysis of eglA places the secreted 34-kDa protein into the glycosyl hydrolases family 5, with highest relatedness (40\% identity) to endoglucanases of the phytopathogenic bacteria Xanthomonas campestris and Ralstonia solanacearum. Transcriptional regulation studied by eglA:: gusA fusion was not significantly affected by cellulose or its breakdown products or by microaerobiosis. Strongest induction (threefold) was obtained in bacteria grown in close vicinity to rice roots. Visible sites of expression were the emergence points of lateral roots and root tips, which are the primary regions of ingress into the root. To study the role in endophytic colonization, eglA was inactivated by transposon mutagenesis. Systemic spreading of the eglA mutant and of a pilAB mutant into the rice shoot could no longer be detected by polymerase chain reaction. Microscopic inspection of infection revealed that the intracellular colonization of root epidermis cells was significantly reduced in the eglA(-) mutant BHE6 compared with the wild type and partially restored in the complementation mutant BHRE2 expressing eglA. This provides evidence that Azoarcus sp. endoglucanase is an important determinant for successful endophytic colonization of rice roots, suggesting an active bacterial colonization process.},
  author       = {Reinhold-Hurek, Barbara and Maes, Tamara and Gemmer, Sabrina and Van Montagu, Marc and Hurek, Thomas},
  issn         = {0894-0282},
  journal      = {MOLECULAR PLANT-MICROBE INTERACTIONS},
  keyword      = {RHIZOBIUM-MELILOTI,ESCHERICHIA-COLI,PSEUDOMONAS-SOLANACEARUM,ERWINIA-CAROTOVORA,CAROTOVORA SUBSP CAROTOVORA,BIOLOGICAL NITROGEN-FIXATION,transcription,pill,Oryza,cellulase,nitrogen fixation,KALLAR GRASS,SUGAR-CANE,NIF GENES,CLONING},
  language     = {eng},
  number       = {2},
  pages        = {181--188},
  title        = {An endoglucanase is involved in infection of rice roots by the not-cellulose-metabolizing endophyte Azoarcus sp. strain BH72},
  url          = {http://dx.doi.org/10.1094/MPMI-19-0181},
  volume       = {19},
  year         = {2006},
}

Chicago
Reinhold-Hurek, Barbara, Tamara Maes, Sabrina Gemmer, Marc Van Montagu, and Thomas Hurek. 2006. “An Endoglucanase Is Involved in Infection of Rice Roots by the Not-cellulose-metabolizing Endophyte Azoarcus Sp. Strain BH72.” Molecular Plant-microbe Interactions 19 (2): 181–188.
APA
Reinhold-Hurek, B., Maes, T., Gemmer, S., Van Montagu, M., & Hurek, T. (2006). An endoglucanase is involved in infection of rice roots by the not-cellulose-metabolizing endophyte Azoarcus sp. strain BH72. MOLECULAR PLANT-MICROBE INTERACTIONS, 19(2), 181–188.
Vancouver
1.
Reinhold-Hurek B, Maes T, Gemmer S, Van Montagu M, Hurek T. An endoglucanase is involved in infection of rice roots by the not-cellulose-metabolizing endophyte Azoarcus sp. strain BH72. MOLECULAR PLANT-MICROBE INTERACTIONS. 2006;19(2):181–8.
MLA
Reinhold-Hurek, Barbara, Tamara Maes, Sabrina Gemmer, et al. “An Endoglucanase Is Involved in Infection of Rice Roots by the Not-cellulose-metabolizing Endophyte Azoarcus Sp. Strain BH72.” MOLECULAR PLANT-MICROBE INTERACTIONS 19.2 (2006): 181–188. Print.