Advanced search
1 file | 870.86 KB Add to list

Human fetal neuroblast and neuroblastoma transcriptome analysis confirms neuroblast origin and highlights neuroblastoma candidate genes

(2006) GENOME BIOLOGY. 7(9).
Author
Organization
Abstract
Background: Neuroblastoma tumor cells are assumed to originate from primitive neuroblasts giving rise to the sympathetic nervous system. Because these precursor cells are not detectable in postnatal life, their transcription profile has remained inaccessible for comparative data mining strategies in neuroblastoma. This study provides the first genome-wide mRNA expression profile of these human fetal sympathetic neuroblasts. To this purpose, small islets of normal neuroblasts were isolated by laser microdissection from human fetal adrenal glands. Results: Expression of catecholamine metabolism genes, and neuronal and neuroendocrine markers in the neuroblasts indicated that the proper cells were microdissected. The similarities in expression profile between normal neuroblasts and malignant neuroblastomas provided strong evidence for the neuroblast origin hypothesis of neuroblastoma. Next, supervised feature selection was used to identify the genes that are differentially expressed in normal neuroblasts versus neuroblastoma tumors. This approach efficiently sifted out genes previously reported in neuroblastoma expression profiling studies; most importantly, it also highlighted a series of genes and pathways previously not mentioned in neuroblastoma biology but that were assumed to be involved in neuroblastoma pathogenesis. Conclusion: This unique dataset adds power to ongoing and future gene expression studies in neuroblastoma and will facilitate the identification of molecular targets for novel therapies. In addition, this neuroblast transcriptome resource could prove useful for the further study of human sympathoadrenal biogenesis.
Keywords
ANAPLASTIC LYMPHOMA KINASE, SYMPATHETIC-NERVOUS-SYSTEM, NEURAL CREST, COPY NUMBER, MICROARRAY ANALYSIS, ALLELIC DELETION, CELL-LINES, EXPRESSION, PROTEIN, CANCER

Downloads

  • De Preter et al 2006 neuroblast profiling.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 870.86 KB

Citation

Please use this url to cite or link to this publication:

MLA
De Preter, Katleen et al. “Human Fetal Neuroblast and Neuroblastoma Transcriptome Analysis Confirms Neuroblast Origin and Highlights Neuroblastoma Candidate Genes.” GENOME BIOLOGY 7.9 (2006): n. pag. Print.
APA
De Preter, K., Vandesompele, J., Heimann, P., Yigit, N., Beckman, S., Schramm, A., Eggert, A., et al. (2006). Human fetal neuroblast and neuroblastoma transcriptome analysis confirms neuroblast origin and highlights neuroblastoma candidate genes. GENOME BIOLOGY, 7(9).
Chicago author-date
De Preter, Katleen, Jo Vandesompele, Pierre Heimann, Nurten Yigit, Siv Beckman, Alexander Schramm, Angelika Eggert, et al. 2006. “Human Fetal Neuroblast and Neuroblastoma Transcriptome Analysis Confirms Neuroblast Origin and Highlights Neuroblastoma Candidate Genes.” Genome Biology 7 (9).
Chicago author-date (all authors)
De Preter, Katleen, Jo Vandesompele, Pierre Heimann, Nurten Yigit, Siv Beckman, Alexander Schramm, Angelika Eggert, Raymond L Stallings, Yves Benoit, Marleen Renard, Anne De Paepe, Genevieve Laureys, Sven Påhlman, and Franki Speleman. 2006. “Human Fetal Neuroblast and Neuroblastoma Transcriptome Analysis Confirms Neuroblast Origin and Highlights Neuroblastoma Candidate Genes.” Genome Biology 7 (9).
Vancouver
1.
De Preter K, Vandesompele J, Heimann P, Yigit N, Beckman S, Schramm A, et al. Human fetal neuroblast and neuroblastoma transcriptome analysis confirms neuroblast origin and highlights neuroblastoma candidate genes. GENOME BIOLOGY. 2006;7(9).
IEEE
[1]
K. De Preter et al., “Human fetal neuroblast and neuroblastoma transcriptome analysis confirms neuroblast origin and highlights neuroblastoma candidate genes,” GENOME BIOLOGY, vol. 7, no. 9, 2006.
@article{369949,
  abstract     = {Background: Neuroblastoma tumor cells are assumed to originate from primitive neuroblasts giving rise to the sympathetic nervous system. Because these precursor cells are not detectable in postnatal life, their transcription profile has remained inaccessible for comparative data mining strategies in neuroblastoma. This study provides the first genome-wide mRNA expression profile of these human fetal sympathetic neuroblasts. To this purpose, small islets of normal neuroblasts were isolated by laser microdissection from human fetal adrenal glands. 
Results: Expression of catecholamine metabolism genes, and neuronal and neuroendocrine markers in the neuroblasts indicated that the proper cells were microdissected. The similarities in expression profile between normal neuroblasts and malignant neuroblastomas provided strong evidence for the neuroblast origin hypothesis of neuroblastoma. Next, supervised feature selection was used to identify the genes that are differentially expressed in normal neuroblasts versus neuroblastoma tumors. This approach efficiently sifted out genes previously reported in neuroblastoma expression profiling studies; most importantly, it also highlighted a series of genes and pathways previously not mentioned in neuroblastoma biology but that were assumed to be involved in neuroblastoma pathogenesis. 
Conclusion: This unique dataset adds power to ongoing and future gene expression studies in neuroblastoma and will facilitate the identification of molecular targets for novel therapies. In addition, this neuroblast transcriptome resource could prove useful for the further study of human sympathoadrenal biogenesis.},
  articleno    = {R84},
  author       = {De Preter, Katleen and Vandesompele, Jo and Heimann, Pierre and Yigit, Nurten and Beckman, Siv and Schramm, Alexander and Eggert, Angelika and Stallings, Raymond L and Benoit, Yves and Renard, Marleen and De Paepe, Anne and Laureys, Genevieve and Påhlman, Sven and Speleman, Franki},
  issn         = {1474-760X},
  journal      = {GENOME BIOLOGY},
  keywords     = {ANAPLASTIC LYMPHOMA KINASE,SYMPATHETIC-NERVOUS-SYSTEM,NEURAL CREST,COPY NUMBER,MICROARRAY ANALYSIS,ALLELIC DELETION,CELL-LINES,EXPRESSION,PROTEIN,CANCER},
  language     = {eng},
  number       = {9},
  pages        = {17},
  title        = {Human fetal neuroblast and neuroblastoma transcriptome analysis confirms neuroblast origin and highlights neuroblastoma candidate genes},
  url          = {http://dx.doi.org/10.1186/gb-2006-7-9-r84},
  volume       = {7},
  year         = {2006},
}

Altmetric
View in Altmetric
Web of Science
Times cited: