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Cloning, sequence analysis and heterologous expression of the Myrothecium gramineum orotidine-5'-monophosphate decarboxylase gene

Sofie De Maeseneire (UGent) , Manu De Groeve (UGent) , Thierry Dauvrin, Marjan De Mey (UGent) , Wim Soetaert (UGent) and Erick Vandamme (UGent)
(2006) FEMS MICROBIOLOGY LETTERS. 261(2). p.262-271
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Abstract
A 2918 bp sequence coding for the orotidine-5'-monophosphate decarboxylase enzyme (OMPD) was isolated from the genome of Myrothecium gramineum. This sequence was analysed and, remarkably, it is the first OMPD gene of a Sordariomycete that has an intron. The gene codes for an enzyme of 282 amino acids. The nucleotide sequence and the amino acid sequence were compared with fungal OMPD sequences. They show the highest similarity to OMPD genes and enzymes of Aspergillus sp., Penicillium sp. and Cladosporium fulvum. The functionality of the gene as a selection marker was proven by complementation of the uracil auxotrophy of Aspergillus nidulans FGSC A722.
Keywords
Ascomycetes, filamentous fungi, selection marker, gene structure, transformation, Aspergillus nidulans, ENCODING OROTIDINE-5'-MONOPHOSPHATE DECARBOXYLASE, OROTIDINE 5'-MONOPHOSPHATE DECARBOXYLASE, PYRG GENE, TRANSFORMATION, MUTANTS, SYSTEM, FAMILY, SITE

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Citation

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MLA
De Maeseneire, Sofie, Manu De Groeve, Thierry Dauvrin, et al. “Cloning, Sequence Analysis and Heterologous Expression of the Myrothecium Gramineum Orotidine-5’-monophosphate Decarboxylase Gene.” FEMS MICROBIOLOGY LETTERS 261.2 (2006): 262–271. Print.
APA
De Maeseneire, S., De Groeve, M., Dauvrin, T., De Mey, M., Soetaert, W., & Vandamme, E. (2006). Cloning, sequence analysis and heterologous expression of the Myrothecium gramineum orotidine-5’-monophosphate decarboxylase gene. FEMS MICROBIOLOGY LETTERS, 261(2), 262–271.
Chicago author-date
De Maeseneire, Sofie, Manu De Groeve, Thierry Dauvrin, Marjan De Mey, Wim Soetaert, and Erick Vandamme. 2006. “Cloning, Sequence Analysis and Heterologous Expression of the Myrothecium Gramineum Orotidine-5’-monophosphate Decarboxylase Gene.” Fems Microbiology Letters 261 (2): 262–271.
Chicago author-date (all authors)
De Maeseneire, Sofie, Manu De Groeve, Thierry Dauvrin, Marjan De Mey, Wim Soetaert, and Erick Vandamme. 2006. “Cloning, Sequence Analysis and Heterologous Expression of the Myrothecium Gramineum Orotidine-5’-monophosphate Decarboxylase Gene.” Fems Microbiology Letters 261 (2): 262–271.
Vancouver
1.
De Maeseneire S, De Groeve M, Dauvrin T, De Mey M, Soetaert W, Vandamme E. Cloning, sequence analysis and heterologous expression of the Myrothecium gramineum orotidine-5’-monophosphate decarboxylase gene. FEMS MICROBIOLOGY LETTERS. 2006;261(2):262–71.
IEEE
[1]
S. De Maeseneire, M. De Groeve, T. Dauvrin, M. De Mey, W. Soetaert, and E. Vandamme, “Cloning, sequence analysis and heterologous expression of the Myrothecium gramineum orotidine-5’-monophosphate decarboxylase gene,” FEMS MICROBIOLOGY LETTERS, vol. 261, no. 2, pp. 262–271, 2006.
@article{368162,
  abstract     = {A 2918 bp sequence coding for the orotidine-5'-monophosphate decarboxylase enzyme (OMPD) was isolated from the genome of Myrothecium gramineum. This sequence was analysed and, remarkably, it is the first OMPD gene of a Sordariomycete that has an intron. The gene codes for an enzyme of 282 amino acids. The nucleotide sequence and the amino acid sequence were compared with fungal OMPD sequences. They show the highest similarity to OMPD genes and enzymes of Aspergillus sp., Penicillium sp. and Cladosporium fulvum. The functionality of the gene as a selection marker was proven by complementation of the uracil auxotrophy of Aspergillus nidulans FGSC A722.},
  author       = {De Maeseneire, Sofie and De Groeve, Manu and Dauvrin, Thierry and De Mey, Marjan and Soetaert, Wim and Vandamme, Erick},
  issn         = {0378-1097},
  journal      = {FEMS MICROBIOLOGY LETTERS},
  keywords     = {Ascomycetes,filamentous fungi,selection marker,gene structure,transformation,Aspergillus nidulans,ENCODING OROTIDINE-5'-MONOPHOSPHATE DECARBOXYLASE,OROTIDINE 5'-MONOPHOSPHATE DECARBOXYLASE,PYRG GENE,TRANSFORMATION,MUTANTS,SYSTEM,FAMILY,SITE},
  language     = {eng},
  number       = {2},
  pages        = {262--271},
  title        = {Cloning, sequence analysis and heterologous expression of the Myrothecium gramineum orotidine-5'-monophosphate decarboxylase gene},
  url          = {http://dx.doi.org/10.1111/j.1574-6968.2006.00373.x},
  volume       = {261},
  year         = {2006},
}

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