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SIAMESE, a plant-specific cell cycle regulator, controls endoreplication onset in Arabidopsis thaliana

(2006) PLANT CELL. 18(11). p.3145-3157
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Abstract
Recessive mutations in the SIAMESE (SIM) gene of Arabidopsis thaliana result in multicellular trichomes harboring individual nuclei with a low ploidy level, a phenotype strikingly different from that of wild-type trichomes, which are single cells with a nuclear DNA content of similar to 16C to 32C. These observations suggested that SIM is required to suppress mitosis as part of the switch to endoreplication in trichomes. Here, we demonstrate that SIM encodes a nuclear-localized 14-kD protein containing a cyclin binding motif and a motif found in ICK/KRP (for Interactors of Cdc2 kinase/Kip-related protein) cell cycle inhibitor proteins. Accordingly, SIM was found to associate with D-type cyclins and CDKA;1. Homologs of SIM were detected in other dicots and in monocots but not in mammals or fungi. SIM proteins are expressed throughout the shoot apical meristem, in leaf primordia, and in the elongation zone of the root and are localized to the nucleus. Plants overexpressing SIM are slow-growing and have narrow leaves and enlarged epidermal cells with an increased DNA content resulting from additional endocycles. We hypothesize that SIM encodes a plant-specific CDK inhibitor with a key function in the mitosis-to-endoreplication transition.
Keywords
EXPRESSION, GENE, INFLORESCENCE, ENDOPOLYPLOIDY, MAIZE ENDOSPERM, BINDING-PROTEINS, TRICHOME DEVELOPMENT, DNA ENDOREDUPLICATION, DEPENDENT KINASE INHIBITORS, RESONANCE ENERGY-TRANSFER

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Chicago
Churchman, Michelle L, Matthew L Brown, Naohiro Kato, Viktor Kirik, Martin Hülskamp, Dirk Inzé, Lieven De Veylder, et al. 2006. “SIAMESE, a Plant-specific Cell Cycle Regulator, Controls Endoreplication Onset in Arabidopsis Thaliana.” Plant Cell 18 (11): 3145–3157.
APA
Churchman, M. L., Brown, M. L., Kato, N., Kirik, V., Hülskamp, M., Inzé, D., De Veylder, L., et al. (2006). SIAMESE, a plant-specific cell cycle regulator, controls endoreplication onset in Arabidopsis thaliana. PLANT CELL, 18(11), 3145–3157.
Vancouver
1.
Churchman ML, Brown ML, Kato N, Kirik V, Hülskamp M, Inzé D, et al. SIAMESE, a plant-specific cell cycle regulator, controls endoreplication onset in Arabidopsis thaliana. PLANT CELL. 2006;18(11):3145–57.
MLA
Churchman, Michelle L, Matthew L Brown, Naohiro Kato, et al. “SIAMESE, a Plant-specific Cell Cycle Regulator, Controls Endoreplication Onset in Arabidopsis Thaliana.” PLANT CELL 18.11 (2006): 3145–3157. Print.
@article{366804,
  abstract     = {Recessive mutations in the SIAMESE (SIM) gene of Arabidopsis thaliana result in multicellular trichomes harboring individual nuclei with a low ploidy level, a phenotype strikingly different from that of wild-type trichomes, which are single cells with a nuclear DNA content of similar to 16C to 32C. These observations suggested that SIM is required to suppress mitosis as part of the switch to endoreplication in trichomes. Here, we demonstrate that SIM encodes a nuclear-localized 14-kD protein containing a cyclin binding motif and a motif found in ICK/KRP (for Interactors of Cdc2 kinase/Kip-related protein) cell cycle inhibitor proteins. Accordingly, SIM was found to associate with D-type cyclins and CDKA;1. Homologs of SIM were detected in other dicots and in monocots but not in mammals or fungi. SIM proteins are expressed throughout the shoot apical meristem, in leaf primordia, and in the elongation zone of the root and are localized to the nucleus. Plants overexpressing SIM are slow-growing and have narrow leaves and enlarged epidermal cells with an increased DNA content resulting from additional endocycles. We hypothesize that SIM encodes a plant-specific CDK inhibitor with a key function in the mitosis-to-endoreplication transition.},
  author       = {Churchman, Michelle L and Brown, Matthew L and Kato, Naohiro and Kirik, Viktor and H{\"u}lskamp, Martin and Inz{\'e}, Dirk and De Veylder, Lieven and Walker, Jason D and Zheng, Zhengui and Oppenheimer, David G and Gwin, Taylor and Churchman, Jason and Larkin, John C},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  keyword      = {EXPRESSION,GENE,INFLORESCENCE,ENDOPOLYPLOIDY,MAIZE ENDOSPERM,BINDING-PROTEINS,TRICHOME DEVELOPMENT,DNA ENDOREDUPLICATION,DEPENDENT KINASE INHIBITORS,RESONANCE ENERGY-TRANSFER},
  language     = {eng},
  number       = {11},
  pages        = {3145--3157},
  title        = {SIAMESE, a plant-specific cell cycle regulator, controls endoreplication onset in Arabidopsis thaliana},
  url          = {http://dx.doi.org/10.1105/tpc.106.044834},
  volume       = {18},
  year         = {2006},
}

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