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Genetical metabolomics of flavonoid biosynthesis in Populus: a case study

Kris Morreel UGent, Geert Goeminne UGent, Veronique Storme UGent, Lieven Sterck UGent, John Ralph, Wouter Coppieters, Peter Breyne UGent, Marijke Steenackers, Michel Georges and Eric Messens UGent, et al. (2006) PLANT JOURNAL. 47(2). p.224-237
abstract
Genetical metabolomics [metabolite profiling combined with quantitative trait locus (QTL) analysis] has been proposed as a new tool to identify loci that control metabolite abundances. This concept was evaluated in a case study with the model tree Populus. Using HPLC, the peak abundances were analyzed of 15 closely related flavonoids present in apical tissues of two full-sib poplar families, Populus deltoides cv. S9-2 x P. nigra cv. Ghoy and P. deltoides cv. S9-2 x P. trichocarpa cv. V24, and correlation and QTL analysis were used to detect flux control points in flavonoid biosynthesis. Four robust metabolite quantitative trait loci (mQTL), associated with rate-limiting steps in flavonoid biosynthesis, were mapped. Each mQTL was involved in the flux control to one or two flavonoids. Based on the identities of the affected metabolites and the flavonoid pathway structure, a tentative function was assigned to three of these mQTL, and the corresponding candidate genes were mapped. The data indicate that the combination of metabolite profiling with QTL analysis is a valuable tool to identify control points in a complex metabolic pathway of closely related compounds.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
METABOLITE CONCENTRATIONS, BARLEY LEAVES, O-METHYLTRANSFERASE, QUANTITATIVE TRAIT LOCI, genetical metabolomics, mQTL, correlation analysis, metabolite profiling, poplar, flavonoids, LINKAGE MAPS, MARKERS, NETWORKS, ENZYMES, PATHWAY, PHLOEM
journal title
PLANT JOURNAL
Plant J.
volume
47
issue
2
pages
224 - 237
Web of Science type
Article
Web of Science id
000238656100005
JCR category
PLANT SCIENCES
JCR impact factor
6.565 (2006)
JCR rank
6/145 (2006)
JCR quartile
1 (2006)
ISSN
0960-7412
DOI
10.1111/j.1365-313X.2006.02786.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
366798
handle
http://hdl.handle.net/1854/LU-366798
date created
2007-05-21 15:09:00
date last changed
2013-10-01 14:07:51
@article{366798,
  abstract     = {Genetical metabolomics [metabolite profiling combined with quantitative trait locus (QTL) analysis] has been proposed as a new tool to identify loci that control metabolite abundances. This concept was evaluated in a case study with the model tree Populus. Using HPLC, the peak abundances were analyzed of 15 closely related flavonoids present in apical tissues of two full-sib poplar families, Populus deltoides cv. S9-2 x P. nigra cv. Ghoy and P. deltoides cv. S9-2 x P. trichocarpa cv. V24, and correlation and QTL analysis were used to detect flux control points in flavonoid biosynthesis. Four robust metabolite quantitative trait loci (mQTL), associated with rate-limiting steps in flavonoid biosynthesis, were mapped. Each mQTL was involved in the flux control to one or two flavonoids. Based on the identities of the affected metabolites and the flavonoid pathway structure, a tentative function was assigned to three of these mQTL, and the corresponding candidate genes were mapped. The data indicate that the combination of metabolite profiling with QTL analysis is a valuable tool to identify control points in a complex metabolic pathway of closely related compounds.},
  author       = {Morreel, Kris and Goeminne, Geert and Storme, Veronique and Sterck, Lieven and Ralph, John and Coppieters, Wouter and Breyne, Peter and Steenackers, Marijke and Georges, Michel and Messens, Eric and Boerjan, Wout},
  issn         = {0960-7412},
  journal      = {PLANT JOURNAL},
  keyword      = {METABOLITE CONCENTRATIONS,BARLEY LEAVES,O-METHYLTRANSFERASE,QUANTITATIVE TRAIT LOCI,genetical metabolomics,mQTL,correlation analysis,metabolite profiling,poplar,flavonoids,LINKAGE MAPS,MARKERS,NETWORKS,ENZYMES,PATHWAY,PHLOEM},
  language     = {eng},
  number       = {2},
  pages        = {224--237},
  title        = {Genetical metabolomics of flavonoid biosynthesis in Populus: a case study},
  url          = {http://dx.doi.org/10.1111/j.1365-313X.2006.02786.x},
  volume       = {47},
  year         = {2006},
}

Chicago
Morreel, Kris, Geert Goeminne, Veronique Storme, Lieven Sterck, John Ralph, Wouter Coppieters, Peter Breyne, et al. 2006. “Genetical Metabolomics of Flavonoid Biosynthesis in Populus: a Case Study.” Plant Journal 47 (2): 224–237.
APA
Morreel, K., Goeminne, G., Storme, V., Sterck, L., Ralph, J., Coppieters, W., Breyne, P., et al. (2006). Genetical metabolomics of flavonoid biosynthesis in Populus: a case study. PLANT JOURNAL, 47(2), 224–237.
Vancouver
1.
Morreel K, Goeminne G, Storme V, Sterck L, Ralph J, Coppieters W, et al. Genetical metabolomics of flavonoid biosynthesis in Populus: a case study. PLANT JOURNAL. 2006;47(2):224–37.
MLA
Morreel, Kris, Geert Goeminne, Veronique Storme, et al. “Genetical Metabolomics of Flavonoid Biosynthesis in Populus: a Case Study.” PLANT JOURNAL 47.2 (2006): 224–237. Print.