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Telomerase activity and hTERT protein expression in meningiomas: an analysis in vivo versus in vitro

Lode Maes UGent, Jean-Pierre Kalala Okito UGent, Maria Cornelissen UGent and Leo De Ridder (2006) ANTICANCER RESEARCH. 26(3B). p.2295-2300
abstract
Background: Telomere length maintenance is essential for tumorigenesis. Most human tumours stabilise their chromosome ends by telomerase, a specialised reverse transcriptase that adds telomeric repeats (TTAGGG) to these ends. The main components of this telomerase complex are a reverse transcriptase (hTERT) and an integral RNA component (hTR). Most typical meningiomas, however, do not have active telomerase, although some express the hTERT component. The aim of this study was to evaluate telomerase activity and its reverse transcriptase for 33 (30 typical and three atypical) meningiomas in vivo and in vitro. Materials and Methods: Telomerase activity was examined by the telomeric repeat amplification protocol (TRAP) assay. The protein, telomerase reverse transcriptase, was visualised by immunohistochemistry. Results: In vivo, telomerase activity was detectable in one out of 30 typical meningiomas and in two out of three atypical meningiomas. hTERT protein expression in vivo was positive in 14 out of 33 (42%) cases. The mean percentage of positive nuclei was 12.9% (SD=21.0). In vitro, 22 out of 33 (66%) meningiomas were positive for hTERT, with a mean percentage of positive nuclei of 31.8% (SD=371). Only four expressed telomerase activity in vitro, from which three had expressed telomerase activity in vivo. A significant association was found for telomerase activity (p < 0.001) and hTERT expression (p < 0.001) in vivo versus in vitro; a significant association was found for hTERT expression and telomerase activity in vivo (p < 0.05) and in vitro (p < 0.05). Conclusion: The results of our study suggest that hTERT expression is an early event in carcinogenesis in contrast to telomerase activity. Fast-proliferating hTERT-positive tumour cells may overgrow in vitro by clonal selection.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
BRAIN-TUMORS, CATALYTIC SUBUNIT, REVERSE-TRANSCRIPTASE, hTERT, meningiomas, telomerase, CELLS, MARKER
journal title
ANTICANCER RESEARCH
Anticancer Res.
volume
26
issue
3B
pages
2295 - 2300
Web of Science type
Article
Web of Science id
000238491000021
JCR category
ONCOLOGY
JCR impact factor
1.479 (2006)
JCR rank
102/125 (2006)
JCR quartile
4 (2006)
ISSN
0250-7005
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
366366
handle
http://hdl.handle.net/1854/LU-366366
alternative location
http://ar.iiarjournals.org/content/26/3B/2295
date created
2007-05-16 15:55:00
date last changed
2016-12-19 15:40:17
@article{366366,
  abstract     = {Background: Telomere length maintenance is essential for tumorigenesis. Most human tumours stabilise their chromosome ends by telomerase, a specialised reverse transcriptase that adds telomeric repeats (TTAGGG) to these ends. The main components of this telomerase complex are a reverse transcriptase (hTERT) and an integral RNA component (hTR). Most typical meningiomas, however, do not have active telomerase, although some express the hTERT component. The aim of this study was to evaluate telomerase activity and its reverse transcriptase for 33 (30 typical and three atypical) meningiomas in vivo and in vitro. Materials and Methods: Telomerase activity was examined by the telomeric repeat amplification protocol (TRAP) assay. The protein, telomerase reverse transcriptase, was visualised by immunohistochemistry. Results: In vivo, telomerase activity was detectable in one out of 30 typical meningiomas and in two out of three atypical meningiomas. hTERT protein expression in vivo was positive in 14 out of 33 (42\%) cases. The mean percentage of positive nuclei was 12.9\% (SD=21.0). In vitro, 22 out of 33 (66\%) meningiomas were positive for hTERT, with a mean percentage of positive nuclei of 31.8\% (SD=371). Only four expressed telomerase activity in vitro, from which three had expressed telomerase activity in vivo. A significant association was found for telomerase activity (p {\textlangle} 0.001) and hTERT expression (p {\textlangle} 0.001) in vivo versus in vitro; a significant association was found for hTERT expression and telomerase activity in vivo (p {\textlangle} 0.05) and in vitro (p {\textlangle} 0.05). Conclusion: The results of our study suggest that hTERT expression is an early event in carcinogenesis in contrast to telomerase activity. Fast-proliferating hTERT-positive tumour cells may overgrow in vitro by clonal selection.},
  author       = {Maes, Lode and Kalala Okito, Jean-Pierre and Cornelissen, Maria and De Ridder, Leo},
  issn         = {0250-7005},
  journal      = {ANTICANCER RESEARCH},
  keyword      = {BRAIN-TUMORS,CATALYTIC SUBUNIT,REVERSE-TRANSCRIPTASE,hTERT,meningiomas,telomerase,CELLS,MARKER},
  language     = {eng},
  number       = {3B},
  pages        = {2295--2300},
  title        = {Telomerase activity and hTERT protein expression in meningiomas: an analysis in vivo versus in vitro},
  url          = {http://ar.iiarjournals.org/content/26/3B/2295},
  volume       = {26},
  year         = {2006},
}

Chicago
Maes, Lode, Jean-Pierre Kalala Okito, Maria Cornelissen, and Leo De Ridder. 2006. “Telomerase Activity and hTERT Protein Expression in Meningiomas: An Analysis in Vivo Versus in Vitro.” Anticancer Research 26 (3B): 2295–2300.
APA
Maes, Lode, Kalala Okito, J.-P., Cornelissen, M., & De Ridder, L. (2006). Telomerase activity and hTERT protein expression in meningiomas: an analysis in vivo versus in vitro. ANTICANCER RESEARCH, 26(3B), 2295–2300.
Vancouver
1.
Maes L, Kalala Okito J-P, Cornelissen M, De Ridder L. Telomerase activity and hTERT protein expression in meningiomas: an analysis in vivo versus in vitro. ANTICANCER RESEARCH. 2006;26(3B):2295–300.
MLA
Maes, Lode, Jean-Pierre Kalala Okito, Maria Cornelissen, et al. “Telomerase Activity and hTERT Protein Expression in Meningiomas: An Analysis in Vivo Versus in Vitro.” ANTICANCER RESEARCH 26.3B (2006): 2295–2300. Print.