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Chemotaxonomical identification of spores of macrofungi: possibilities of Raman spectroscopy

Kris De Gussem (UGent) , Peter Vandenabeele (UGent) , Annemieke Verbeken (UGent) and Luc Moens (UGent)
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Abstract
Confocal Raman spectroscopy is a non-destructive analytical method which is useful to obtain detailed information about the molecular composition of biological samples. Its high spatial resolution was used to collect spectra of single basidiospores of macrofungi of the genera Collybia, Gymnopus, Laccaria, Lactarius, Mycena and Russula. These spectra can be divided into three major taxon-related groups, with general compositional differences, such as the relative amount of lipids compared to proteins. In this study, collapsing of thin-walled spores during storage was often observed, a phenomenon which has been given little attention in the literature. The Raman spectra are treated with different chemometric preprocessing techniques, including Savitsky-Golay, standard normal variate (SNV) preprocessing and extended multiplicative scatter correction (EMSC). By using linear discriminant analysis, approximately 90% of the spectra can be assigned to the correct genus, but identification on the species level was not possible.
Keywords
Raman spectroscopy, basidiomycotina, chemotaxonomy, genus identification, RAPID IDENTIFICATION, INFRARED-SPECTROSCOPY, MASS-SPECTROMETRY, BACTERIAL, STRAINS, CELLS, MICROSPECTROSCOPY, CLASSIFICATION, MICROORGANISMS, FUNGI

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Chicago
De Gussem, Kris, Peter Vandenabeele, Annemieke Verbeken, and Luc Moens. 2007. “Chemotaxonomical Identification of Spores of Macrofungi: Possibilities of Raman Spectroscopy.” Analytical and Bioanalytical Chemistry 387 (8): 2823–2832.
APA
De Gussem, K., Vandenabeele, P., Verbeken, A., & Moens, L. (2007). Chemotaxonomical identification of spores of macrofungi: possibilities of Raman spectroscopy. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 387(8), 2823–2832.
Vancouver
1.
De Gussem K, Vandenabeele P, Verbeken A, Moens L. Chemotaxonomical identification of spores of macrofungi: possibilities of Raman spectroscopy. ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 2007;387(8):2823–32.
MLA
De Gussem, Kris, Peter Vandenabeele, Annemieke Verbeken, et al. “Chemotaxonomical Identification of Spores of Macrofungi: Possibilities of Raman Spectroscopy.” ANALYTICAL AND BIOANALYTICAL CHEMISTRY 387.8 (2007): 2823–2832. Print.
@article{365129,
  abstract     = {Confocal Raman spectroscopy is a non-destructive analytical method which is useful to obtain detailed information about the molecular composition of biological samples. Its high spatial resolution was used to collect spectra of single basidiospores of macrofungi of the genera Collybia, Gymnopus, Laccaria, Lactarius, Mycena and Russula. These spectra can be divided into three major taxon-related groups, with general compositional differences, such as the relative amount of lipids compared to proteins. In this study, collapsing of thin-walled spores during storage was often observed, a phenomenon which has been given little attention in the literature. The Raman spectra are treated with different chemometric preprocessing techniques, including Savitsky-Golay, standard normal variate (SNV) preprocessing and extended multiplicative scatter correction (EMSC). By using linear discriminant analysis, approximately 90\% of the spectra can be assigned to the correct genus, but identification on the species level was not possible.},
  author       = {De Gussem, Kris and Vandenabeele, Peter and Verbeken, Annemieke and Moens, Luc},
  issn         = {1618-2642},
  journal      = {ANALYTICAL AND BIOANALYTICAL CHEMISTRY},
  keyword      = {Raman spectroscopy,basidiomycotina,chemotaxonomy,genus identification,RAPID IDENTIFICATION,INFRARED-SPECTROSCOPY,MASS-SPECTROMETRY,BACTERIAL,STRAINS,CELLS,MICROSPECTROSCOPY,CLASSIFICATION,MICROORGANISMS,FUNGI},
  language     = {eng},
  number       = {8},
  pages        = {2823--2832},
  title        = {Chemotaxonomical identification of spores of macrofungi: possibilities of Raman spectroscopy},
  url          = {http://dx.doi.org/10.1007/s00216-007-1150-1},
  volume       = {387},
  year         = {2007},
}

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