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Chemotaxonomical identification of spores of macrofungi: possibilities of Raman spectroscopy

Kris De Gussem UGent, Peter Vandenabeele UGent, Annemieke Verbeken UGent and Luc Moens UGent (2007) ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 387(8). p.2823-2832
abstract
Confocal Raman spectroscopy is a non-destructive analytical method which is useful to obtain detailed information about the molecular composition of biological samples. Its high spatial resolution was used to collect spectra of single basidiospores of macrofungi of the genera Collybia, Gymnopus, Laccaria, Lactarius, Mycena and Russula. These spectra can be divided into three major taxon-related groups, with general compositional differences, such as the relative amount of lipids compared to proteins. In this study, collapsing of thin-walled spores during storage was often observed, a phenomenon which has been given little attention in the literature. The Raman spectra are treated with different chemometric preprocessing techniques, including Savitsky-Golay, standard normal variate (SNV) preprocessing and extended multiplicative scatter correction (EMSC). By using linear discriminant analysis, approximately 90% of the spectra can be assigned to the correct genus, but identification on the species level was not possible.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Raman spectroscopy, basidiomycotina, chemotaxonomy, genus identification, RAPID IDENTIFICATION, INFRARED-SPECTROSCOPY, MASS-SPECTROMETRY, BACTERIAL, STRAINS, CELLS, MICROSPECTROSCOPY, CLASSIFICATION, MICROORGANISMS, FUNGI
journal title
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Anal. Bioanal. Chem.
volume
387
issue
8
pages
2823 - 2832
Web of Science type
Article
Web of Science id
000245123600026
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
2.867 (2007)
JCR rank
16/69 (2007)
JCR quartile
1 (2007)
ISSN
1618-2642
DOI
10.1007/s00216-007-1150-1
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
365129
handle
http://hdl.handle.net/1854/LU-365129
date created
2007-05-07 10:46:00
date last changed
2018-01-29 12:18:52
@article{365129,
  abstract     = {Confocal Raman spectroscopy is a non-destructive analytical method which is useful to obtain detailed information about the molecular composition of biological samples. Its high spatial resolution was used to collect spectra of single basidiospores of macrofungi of the genera Collybia, Gymnopus, Laccaria, Lactarius, Mycena and Russula. These spectra can be divided into three major taxon-related groups, with general compositional differences, such as the relative amount of lipids compared to proteins. In this study, collapsing of thin-walled spores during storage was often observed, a phenomenon which has been given little attention in the literature. The Raman spectra are treated with different chemometric preprocessing techniques, including Savitsky-Golay, standard normal variate (SNV) preprocessing and extended multiplicative scatter correction (EMSC). By using linear discriminant analysis, approximately 90\% of the spectra can be assigned to the correct genus, but identification on the species level was not possible.},
  author       = {De Gussem, Kris and Vandenabeele, Peter and Verbeken, Annemieke and Moens, Luc},
  issn         = {1618-2642},
  journal      = {ANALYTICAL AND BIOANALYTICAL CHEMISTRY},
  keyword      = {Raman spectroscopy,basidiomycotina,chemotaxonomy,genus identification,RAPID IDENTIFICATION,INFRARED-SPECTROSCOPY,MASS-SPECTROMETRY,BACTERIAL,STRAINS,CELLS,MICROSPECTROSCOPY,CLASSIFICATION,MICROORGANISMS,FUNGI},
  language     = {eng},
  number       = {8},
  pages        = {2823--2832},
  title        = {Chemotaxonomical identification of spores of macrofungi: possibilities of Raman spectroscopy},
  url          = {http://dx.doi.org/10.1007/s00216-007-1150-1},
  volume       = {387},
  year         = {2007},
}

Chicago
De Gussem, Kris, Peter Vandenabeele, Annemieke Verbeken, and Luc Moens. 2007. “Chemotaxonomical Identification of Spores of Macrofungi: Possibilities of Raman Spectroscopy.” Analytical and Bioanalytical Chemistry 387 (8): 2823–2832.
APA
De Gussem, K., Vandenabeele, P., Verbeken, A., & Moens, L. (2007). Chemotaxonomical identification of spores of macrofungi: possibilities of Raman spectroscopy. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 387(8), 2823–2832.
Vancouver
1.
De Gussem K, Vandenabeele P, Verbeken A, Moens L. Chemotaxonomical identification of spores of macrofungi: possibilities of Raman spectroscopy. ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 2007;387(8):2823–32.
MLA
De Gussem, Kris, Peter Vandenabeele, Annemieke Verbeken, et al. “Chemotaxonomical Identification of Spores of Macrofungi: Possibilities of Raman Spectroscopy.” ANALYTICAL AND BIOANALYTICAL CHEMISTRY 387.8 (2007): 2823–2832. Print.