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Somatic cytokinesis and pollen maturation in Arabidopsis depend on TPLATE, which has domains similar to coat proteins

Daniël Van Damme (UGent) , Silvie Coutuer (UGent) , Riet De Rycke (UGent) , François-Yves Bouget, Dirk Inzé (UGent) and Danny Geelen (UGent)
(2006) PLANT CELL. 18(12). p.3502-3518
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Abstract
TPLATE was previously identified as a potential cytokinesis protein targeted to the cell plate. Disruption of TPLATE in Arabidopsis thaliana leads to the production of shriveled pollen unable to germinate. Vesicular compartmentalization of the mature pollen is dramatically altered, and large callose deposits accumulate near the intine cell wall layer. Green fluorescent protein (GFP)-tagged TPLATE expression under the control of the pollen promoter Lat52 complements the phenotype. Downregulation of TPLATE in Arabidopsis seedlings and tobacco ( Nicotiana tabacum) BY-2 suspension cells results in crooked cell walls and cell plates that fail to insert into the mother wall. Besides accumulating at the cell plate, GFP-fused TPLATE is temporally targeted to a narrow zone at the cell cortex where the cell plate connects to the mother wall. TPLATE-GFP also localizes to subcellular structures that accumulate at the pollen tube exit site in germinating pollen. Ectopic callose depositions observed in mutant pollen also occur in RNA interference plants, suggesting that TPLATE is implicated in cell wall modification. TPLATE contains domains similar to adaptin and b-COP coat proteins. These data suggest that TPLATE functions in vesicle-trafficking events required for site-specific cell wall modifications during pollen germination and for anchoring of the cell plate to the mother wall at the correct cortical position.
Keywords
DYNAMIN-LIKE PROTEIN, CELL PLATE FORMATION, PLASMA-MEMBRANE, HIGHER-PLANTS, CELLULOSE BIOSYNTHESIS, PREPROPHASE BANDS, GENE-EXPRESSION, DIVISION SITE, TOBACCO BY-2, GROWTH

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Citation

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MLA
Van Damme, Daniël et al. “Somatic Cytokinesis and Pollen Maturation in Arabidopsis Depend on TPLATE, Which Has Domains Similar to Coat Proteins.” PLANT CELL 18.12 (2006): 3502–3518. Print.
APA
Van Damme, Daniël, Coutuer, S., De Rycke, R., Bouget, F.-Y., Inzé, D., & Geelen, D. (2006). Somatic cytokinesis and pollen maturation in Arabidopsis depend on TPLATE, which has domains similar to coat proteins. PLANT CELL, 18(12), 3502–3518.
Chicago author-date
Van Damme, Daniël, Silvie Coutuer, Riet De Rycke, François-Yves Bouget, Dirk Inzé, and Danny Geelen. 2006. “Somatic Cytokinesis and Pollen Maturation in Arabidopsis Depend on TPLATE, Which Has Domains Similar to Coat Proteins.” Plant Cell 18 (12): 3502–3518.
Chicago author-date (all authors)
Van Damme, Daniël, Silvie Coutuer, Riet De Rycke, François-Yves Bouget, Dirk Inzé, and Danny Geelen. 2006. “Somatic Cytokinesis and Pollen Maturation in Arabidopsis Depend on TPLATE, Which Has Domains Similar to Coat Proteins.” Plant Cell 18 (12): 3502–3518.
Vancouver
1.
Van Damme D, Coutuer S, De Rycke R, Bouget F-Y, Inzé D, Geelen D. Somatic cytokinesis and pollen maturation in Arabidopsis depend on TPLATE, which has domains similar to coat proteins. PLANT CELL. 2006;18(12):3502–18.
IEEE
[1]
D. Van Damme, S. Coutuer, R. De Rycke, F.-Y. Bouget, D. Inzé, and D. Geelen, “Somatic cytokinesis and pollen maturation in Arabidopsis depend on TPLATE, which has domains similar to coat proteins,” PLANT CELL, vol. 18, no. 12, pp. 3502–3518, 2006.
@article{364891,
  abstract     = {TPLATE was previously identified as a potential cytokinesis protein targeted to the cell plate. Disruption of TPLATE in Arabidopsis thaliana leads to the production of shriveled pollen unable to germinate. Vesicular compartmentalization of the mature pollen is dramatically altered, and large callose deposits accumulate near the intine cell wall layer. Green fluorescent protein (GFP)-tagged TPLATE expression under the control of the pollen promoter Lat52 complements the phenotype. Downregulation of TPLATE in Arabidopsis seedlings and tobacco ( Nicotiana tabacum) BY-2 suspension cells results in crooked cell walls and cell plates that fail to insert into the mother wall. Besides accumulating at the cell plate, GFP-fused TPLATE is temporally targeted to a narrow zone at the cell cortex where the cell plate connects to the mother wall. TPLATE-GFP also localizes to subcellular structures that accumulate at the pollen tube exit site in germinating pollen. Ectopic callose depositions observed in mutant pollen also occur in RNA interference plants, suggesting that TPLATE is implicated in cell wall modification. TPLATE contains domains similar to adaptin and b-COP coat proteins. These data suggest that TPLATE functions in vesicle-trafficking events required for site-specific cell wall modifications during pollen germination and for anchoring of the cell plate to the mother wall at the correct cortical position.},
  author       = {Van Damme, Daniël and Coutuer, Silvie and De Rycke, Riet and Bouget, François-Yves and Inzé, Dirk and Geelen, Danny},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  keywords     = {DYNAMIN-LIKE PROTEIN,CELL PLATE FORMATION,PLASMA-MEMBRANE,HIGHER-PLANTS,CELLULOSE BIOSYNTHESIS,PREPROPHASE BANDS,GENE-EXPRESSION,DIVISION SITE,TOBACCO BY-2,GROWTH},
  language     = {eng},
  number       = {12},
  pages        = {3502--3518},
  title        = {Somatic cytokinesis and pollen maturation in Arabidopsis depend on TPLATE, which has domains similar to coat proteins},
  url          = {http://dx.doi.org/10.1105/tpc.106.040923},
  volume       = {18},
  year         = {2006},
}

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