Advanced search
1 file | 772.44 KB

Porous gelatin hydrogels, 1: cryogenic formation and structure analysis

Sandra Van Vlierberghe (UGent) , Veerle Cnudde (UGent) , Peter Dubruel (UGent) , Bert Masschaele (UGent) , An Cosijns (UGent) , Ilse De Paepe, Patric Jacobs (UGent) , Luc Van Hoorebeke (UGent) , Jean Paul Remon (UGent) and Etienne Schacht (UGent)
(2007) BIOMACROMOLECULES. 8(2). p.331-337
Author
Organization
Abstract
In the present work, porous gelatin scaffolds were prepared by cryogenic treatment of a chemically cross-linked gelatin hydrogel, followed by removal of the ice crystals formed through lyophilization. This technique often leads to porous gels with a less porous skin. A simple method has been developed to solve this problem. The present study demonstrates that the hydrogel pore size decreased with an increasing gelatin concentration and with an increasing cooling rate of the gelatin hydrogel. Variation of the cryogenic parameters applied also enabled us to develop scaffolds with different pore morphologies (spherical versus transversal channel-like pores). In our opinion, this is the first paper in which temperature gradients during controlled cryogenic treatment were applied to induce a pore size gradient in gelatin hydrogels. With a newly designed cryo-unit, temperature gradients of 10 and 30 degrees C were implemented during the freezing step, resulting in scaffolds with average pore diameters of, respectively, +/- 116 and +/- 330 mu m. In both cases, the porosity and pore size decreased gradually through the scaffolds. Pore size and structure analysis of the matrices was accomplished through a combination of microcomputed tomography using different software packages (mu CTanalySIS and Octopus), scanning electron microscopy analysis, and helium pycnometry.
Keywords
COLLAGEN-GAG SCAFFOLDS, MICRO-COMPUTED TOMOGRAPHY, PORE STRUCTURE, IN-VITRO, SPONGES, SIZE, CHONDROCYTES, FABRICATION, MATRIX, VIVO

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 772.44 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Van Vlierberghe, Sandra, Veerle Cnudde, Peter Dubruel, Bert Masschaele, An Cosijns, Ilse De Paepe, Patric Jacobs, Luc Van Hoorebeke, Jean Paul Remon, and Etienne Schacht. 2007. “Porous Gelatin Hydrogels, 1: Cryogenic Formation and Structure Analysis.” Biomacromolecules 8 (2): 331–337.
APA
Van Vlierberghe, Sandra, Cnudde, V., Dubruel, P., Masschaele, B., Cosijns, A., De Paepe, I., Jacobs, P., et al. (2007). Porous gelatin hydrogels, 1: cryogenic formation and structure analysis. BIOMACROMOLECULES, 8(2), 331–337.
Vancouver
1.
Van Vlierberghe S, Cnudde V, Dubruel P, Masschaele B, Cosijns A, De Paepe I, et al. Porous gelatin hydrogels, 1: cryogenic formation and structure analysis. BIOMACROMOLECULES. 2007;8(2):331–7.
MLA
Van Vlierberghe, Sandra, Veerle Cnudde, Peter Dubruel, et al. “Porous Gelatin Hydrogels, 1: Cryogenic Formation and Structure Analysis.” BIOMACROMOLECULES 8.2 (2007): 331–337. Print.
@article{364889,
  abstract     = {In the present work, porous gelatin scaffolds were prepared by cryogenic treatment of a chemically cross-linked gelatin hydrogel, followed by removal of the ice crystals formed through lyophilization. This technique often leads to porous gels with a less porous skin. A simple method has been developed to solve this problem. The present study demonstrates that the hydrogel pore size decreased with an increasing gelatin concentration and with an increasing cooling rate of the gelatin hydrogel. Variation of the cryogenic parameters applied also enabled us to develop scaffolds with different pore morphologies (spherical versus transversal channel-like pores). In our opinion, this is the first paper in which temperature gradients during controlled cryogenic treatment were applied to induce a pore size gradient in gelatin hydrogels. With a newly designed cryo-unit, temperature gradients of 10 and 30 degrees C were implemented during the freezing step, resulting in scaffolds with average pore diameters of, respectively, +/- 116 and +/- 330 mu m. In both cases, the porosity and pore size decreased gradually through the scaffolds. Pore size and structure analysis of the matrices was accomplished through a combination of microcomputed tomography using different software packages (mu CTanalySIS and Octopus), scanning electron microscopy analysis, and helium pycnometry.},
  author       = {Van Vlierberghe, Sandra and Cnudde, Veerle and Dubruel, Peter and Masschaele, Bert and Cosijns, An and De Paepe, Ilse and Jacobs, Patric and Van Hoorebeke, Luc and Remon, Jean Paul and Schacht, Etienne},
  issn         = {1525-7797},
  journal      = {BIOMACROMOLECULES},
  keyword      = {COLLAGEN-GAG SCAFFOLDS,MICRO-COMPUTED TOMOGRAPHY,PORE STRUCTURE,IN-VITRO,SPONGES,SIZE,CHONDROCYTES,FABRICATION,MATRIX,VIVO},
  language     = {eng},
  number       = {2},
  pages        = {331--337},
  title        = {Porous gelatin hydrogels, 1: cryogenic formation and structure analysis},
  url          = {http://dx.doi.org/10.1021/bm060684o},
  volume       = {8},
  year         = {2007},
}

Altmetric
View in Altmetric
Web of Science
Times cited: