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Altered gut transcriptome in spondyloarthropathy

Debby Laukens UGent, Harald Peeters UGent, Bert Vander Cruyssen UGent, Tom Boonefaes, Dirk Elewaut UGent, Filip De Keyser UGent, Herman Mielants, Claude Cuvelier, Eric Veys, Kenny Knecht, et al. (2006) ANNALS OF THE RHEUMATIC DISEASES. 65(10). p.1293-1300
abstract
Background: Intestinal inflammation is a common feature of spondyloarthropathy (SpA) and Crohn's disease. Inflammation is manifested clinically in Crohn's disease and subclinically in SpA. However, a fraction of patients with SpA develops overt Crohn's disease. Aims: To investigate whether subclinical gut lesions in patients with SpA are associated with transcriptome changes comparable to those seen in Crohn's disease and to examine global gene expression in non-inflamed colon biopsy specimens and screen patients for differentially expressed genes. Methods: Macroarray analysis was used as an initial genomewide screen for selecting a comprehensive set of genes relevant to Crohn's disease and SpA. This led to the identification of 2625 expressed sequence tags that are differentially expressed in the colon of patients with Crohn's disease or SpA. These clones, with appropriate controls (6779 in total), were used to construct a glass-based microarray, which was then used to analyse colon biopsy specimens from 15 patients with SpA, 11 patients with Crohn's disease and 10 controls. Results: 95 genes were identified as differentially expressed in patients with SpA having a history of subclinical chronic gut inflammation and also in patients with Crohn's disease. Principal component analysis of this filtered set of genes successfully distinguished colon biopsy specimens from the three groups studied. Patients with SpA having subclinical chronic gut inflammation cluster together and are more related to those with Crohn's disease. Conclusion: The transcriptome in the intestine of patients with SpA differs from that of controls. Moreover, these gene changes are comparable to those seen in patients with Crohn's disease, confirming initial clinical observations. On the basis of these findings, new ( genetic) markers for detection of early Crohn's disease in patients with SpA can be considered.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
INFLAMMATORY-BOWEL-DISEASE, AFFECTED RELATIVE PAIRS, CROHNS-DISEASE, ULCERATIVE-COLITIS, GENE-EXPRESSION, INTESTINAL INFLAMMATION, SUSCEPTIBILITY LOCI, COLONIC MUCOSA, GENOME SCAN, EVOLUTION
journal title
ANNALS OF THE RHEUMATIC DISEASES
Ann. Rheum. Dis.
volume
65
issue
10
pages
1293 - 1300
Web of Science type
Article
Web of Science id
000240508600007
JCR category
RHEUMATOLOGY
JCR impact factor
5.767 (2006)
JCR rank
2/23 (2006)
JCR quartile
1 (2006)
ISSN
0003-4967
DOI
10.1136/ard.2005.047738
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
364459
handle
http://hdl.handle.net/1854/LU-364459
date created
2006-10-12 14:33:00
date last changed
2016-12-19 15:40:28
@article{364459,
  abstract     = {Background: Intestinal inflammation is a common feature of spondyloarthropathy (SpA) and Crohn's disease. Inflammation is manifested clinically in Crohn's disease and subclinically in SpA. However, a fraction of patients with SpA develops overt Crohn's disease.
Aims: To investigate whether subclinical gut lesions in patients with SpA are associated with transcriptome changes comparable to those seen in Crohn's disease and to examine global gene expression in non-inflamed colon biopsy specimens and screen patients for differentially expressed genes.
Methods: Macroarray analysis was used as an initial genomewide screen for selecting a comprehensive set of genes relevant to Crohn's disease and SpA. This led to the identification of 2625 expressed sequence tags that are differentially expressed in the colon of patients with Crohn's disease or SpA. These clones, with appropriate controls (6779 in total), were used to construct a glass-based microarray, which was then used to analyse colon biopsy specimens from 15 patients with SpA, 11 patients with Crohn's disease and 10 controls.
Results: 95 genes were identified as differentially expressed in patients with SpA having a history of subclinical chronic gut inflammation and also in patients with Crohn's disease. Principal component analysis of this filtered set of genes successfully distinguished colon biopsy specimens from the three groups studied. Patients with SpA having subclinical chronic gut inflammation cluster together and are more related to those with Crohn's disease.
Conclusion: The transcriptome in the intestine of patients with SpA differs from that of controls. Moreover, these gene changes are comparable to those seen in patients with Crohn's disease, confirming initial clinical observations. On the basis of these findings, new ( genetic) markers for detection of early Crohn's disease in patients with SpA can be considered.},
  author       = {Laukens, Debby and Peeters, Harald and Vander Cruyssen, Bert and Boonefaes, Tom and Elewaut, Dirk and De Keyser, Filip and Mielants, Herman and Cuvelier, Claude and Veys, Eric and Knecht, Kenny and Van Hummelen, P and Remaut, Erik and Steidler, Lothar and De Vos, Martine and Rottiers, Pieter},
  issn         = {0003-4967},
  journal      = {ANNALS OF THE RHEUMATIC DISEASES},
  keyword      = {INFLAMMATORY-BOWEL-DISEASE,AFFECTED RELATIVE PAIRS,CROHNS-DISEASE,ULCERATIVE-COLITIS,GENE-EXPRESSION,INTESTINAL INFLAMMATION,SUSCEPTIBILITY LOCI,COLONIC MUCOSA,GENOME SCAN,EVOLUTION},
  language     = {eng},
  number       = {10},
  pages        = {1293--1300},
  title        = {Altered gut transcriptome in spondyloarthropathy},
  url          = {http://dx.doi.org/10.1136/ard.2005.047738},
  volume       = {65},
  year         = {2006},
}

Chicago
Laukens, Debby, Harald Peeters, Bert Vander Cruyssen, Tom Boonefaes, Dirk Elewaut, Filip De Keyser, Herman Mielants, et al. 2006. “Altered Gut Transcriptome in Spondyloarthropathy.” Annals of the Rheumatic Diseases 65 (10): 1293–1300.
APA
Laukens, D., Peeters, H., Vander Cruyssen, B., Boonefaes, T., Elewaut, D., De Keyser, F., Mielants, H., et al. (2006). Altered gut transcriptome in spondyloarthropathy. ANNALS OF THE RHEUMATIC DISEASES, 65(10), 1293–1300.
Vancouver
1.
Laukens D, Peeters H, Vander Cruyssen B, Boonefaes T, Elewaut D, De Keyser F, et al. Altered gut transcriptome in spondyloarthropathy. ANNALS OF THE RHEUMATIC DISEASES. 2006;65(10):1293–300.
MLA
Laukens, Debby, Harald Peeters, Bert Vander Cruyssen, et al. “Altered Gut Transcriptome in Spondyloarthropathy.” ANNALS OF THE RHEUMATIC DISEASES 65.10 (2006): 1293–1300. Print.