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In vitro permeation of chromium species through porcine and human skin as determined by capillary electrophoresis-inductively coupled plasma-sector field mass spectrometry

Veerle Van Lierde, Cyrille C Chéry, Nathalie Roche, Stan Monstrey UGent, Luc Moens UGent and Frank Vanhaecke UGent (2006) ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 384(2). p.378-384
abstract
Since the species that trigger chromium allergy are not yet known, it is important to gain more of an insight into the mechanism of chromium transport through the skin and into the relationship between chromium allergy and chromium species. In vitro permeation studies with porcine and human skin were performed using a Franz static diffusion cell. Investigations attempted to elucidate (i) which Cr compounds are able to permeate through skin, (ii) the influence the Cr concentration in the donor solution has on the Cr permeation, and (iii) the effect that the time of exposure to the donor solution has on Cr permeation. Capillary electrophoresis hyphenated to inductively coupled plasma-sector field mass spectrometry (CE-ICP-SFMS) was used to separate and quantify the Cr species in the receptor fluid. 50 mmol L-1 phosphate buffer (pH 2.5) was used for CE separation, and two different electrophoretic runs were carried out (in the positive and negative modes). Pneumatic nebulization (PN)-ICP-SFMS was used in order to quantify the total amount of Cr absorbed by the skin after microwave-assisted acid digestion of the tissue. Cr(VI) was found to pass most easily through the skin. Nevertheless, Cr(VI) was also shown to be absorbed more efficiently by the skin than Cr(III), an observation attributed to a more pronounced rejection of the positively charged Cr(III) ions by the skin barrier. These results were in good agreement with in vitro permeation studies previously reported in the literature in which other analytical techniques were used. Differences observed in the permeation of Cr following the application of aqueous Cr donor solutions and Cr-containing simulated sweat donor solutions are also described.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CE-ICP-MS, chromium, porcine skin, human skin, simulated sweat, INVITRO PERCUTANEOUS-ABSORPTION, CONTACT-DERMATITIS, HEXAVALENT CHROMIUM, GUINEA PIG, PENETRATION, ALLERGY, INVIVO, TRIVALENT, MODEL, 51CR
journal title
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Anal. Bioanal. Chem.
volume
384
issue
2
pages
378 - 384
Web of Science type
Article
Web of Science id
000234299600009
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
2.591 (2006)
JCR rank
17/68 (2006)
JCR quartile
1 (2006)
ISSN
1618-2642
DOI
10.1007/s00216-005-0226-z
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
364097
handle
http://hdl.handle.net/1854/LU-364097
date created
2006-02-23 15:50:00
date last changed
2012-11-12 11:39:52
@article{364097,
  abstract     = {Since the species that trigger chromium allergy are not yet known, it is important to gain more of an insight into the mechanism of chromium transport through the skin and into the relationship between chromium allergy and chromium species. In vitro permeation studies with porcine and human skin were performed using a Franz static diffusion cell. Investigations attempted to elucidate (i) which Cr compounds are able to permeate through skin, (ii) the influence the Cr concentration in the donor solution has on the Cr permeation, and (iii) the effect that the time of exposure to the donor solution has on Cr permeation. Capillary electrophoresis hyphenated to inductively coupled plasma-sector field mass spectrometry (CE-ICP-SFMS) was used to separate and quantify the Cr species in the receptor fluid. 50 mmol L-1 phosphate buffer (pH 2.5) was used for CE separation, and two different electrophoretic runs were carried out (in the positive and negative modes). Pneumatic nebulization (PN)-ICP-SFMS was used in order to quantify the total amount of Cr absorbed by the skin after microwave-assisted acid digestion of the tissue. Cr(VI) was found to pass most easily through the skin. Nevertheless, Cr(VI) was also shown to be absorbed more efficiently by the skin than Cr(III), an observation attributed to a more pronounced rejection of the positively charged Cr(III) ions by the skin barrier. These results were in good agreement with in vitro permeation studies previously reported in the literature in which other analytical techniques were used. Differences observed in the permeation of Cr following the application of aqueous Cr donor solutions and Cr-containing simulated sweat donor solutions are also described.},
  author       = {Van Lierde, Veerle and Ch{\'e}ry, Cyrille C and Roche, Nathalie and Monstrey, Stan and Moens, Luc and Vanhaecke, Frank},
  issn         = {1618-2642},
  journal      = {ANALYTICAL AND BIOANALYTICAL CHEMISTRY},
  keyword      = {CE-ICP-MS,chromium,porcine skin,human skin,simulated sweat,INVITRO PERCUTANEOUS-ABSORPTION,CONTACT-DERMATITIS,HEXAVALENT CHROMIUM,GUINEA PIG,PENETRATION,ALLERGY,INVIVO,TRIVALENT,MODEL,51CR},
  language     = {eng},
  number       = {2},
  pages        = {378--384},
  title        = {In vitro permeation of chromium species through porcine and human skin as determined by capillary electrophoresis-inductively coupled plasma-sector field mass spectrometry},
  url          = {http://dx.doi.org/10.1007/s00216-005-0226-z},
  volume       = {384},
  year         = {2006},
}

Chicago
Van Lierde, Veerle, Cyrille C Chéry, Nathalie Roche, Stan Monstrey, Luc Moens, and Frank Vanhaecke. 2006. “In Vitro Permeation of Chromium Species Through Porcine and Human Skin as Determined by Capillary Electrophoresis-inductively Coupled Plasma-sector Field Mass Spectrometry.” Analytical and Bioanalytical Chemistry 384 (2): 378–384.
APA
Van Lierde, V., Chéry, C. C., Roche, N., Monstrey, S., Moens, L., & Vanhaecke, F. (2006). In vitro permeation of chromium species through porcine and human skin as determined by capillary electrophoresis-inductively coupled plasma-sector field mass spectrometry. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 384(2), 378–384.
Vancouver
1.
Van Lierde V, Chéry CC, Roche N, Monstrey S, Moens L, Vanhaecke F. In vitro permeation of chromium species through porcine and human skin as determined by capillary electrophoresis-inductively coupled plasma-sector field mass spectrometry. ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 2006;384(2):378–84.
MLA
Van Lierde, Veerle, Cyrille C Chéry, Nathalie Roche, et al. “In Vitro Permeation of Chromium Species Through Porcine and Human Skin as Determined by Capillary Electrophoresis-inductively Coupled Plasma-sector Field Mass Spectrometry.” ANALYTICAL AND BIOANALYTICAL CHEMISTRY 384.2 (2006): 378–384. Print.