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11C-PET imaging reveals transport dynamics and sectorial plasticity of oak phloem after girdling

Veerle De Schepper, Jonas Bühler, Michael Thorpe, Gerhard Roeb, Gregor Huber, Dagmar van Dusschoten, Siegfried Jahnke and Kathy Steppe UGent (2013) FRONTIERS IN PLANT SCIENCE. 4.
abstract
Carbon transport processes in plants can be followed non-invasively by repeated application of the short-lived positron-emitting radioisotope 11C, a technique which has rarely been used with trees. Recently, positron emission tomography (PET) allowing 3D visualisation has been adapted for use with plants. To investigate the effects of stem girdling on the flow of assimilates, leaves on first order branches of two-year-old oak (Quercus robur L.) trees were labelled with 11C by supplying 11CO2-gas to a leaf cuvette. Magnetic resonance imaging gave an indication of the plant structure, while PET registered the tracer flow in a stem region downstream from the labelled branches. After repeated pulse labelling, phloem translocation was shown to be sectorial in the stem: leaf orthostichy determined the position of the phloem sieve tubes containing labelled 11C. The observed pathway remained unchanged for days. Tracer time-series derived from each pulse and analysed with a mechanistic model showed for two adjacent heights in the stem a similar velocity but different loss of recent assimilates. With either complete or partial girdling of bark within the monitored region, transport immediately stopped and then resumed in a new location in the stem cross-section, demonstrating the plasticity of sectoriality. One day after partial girdling, the loss of tracer along the interrupted transport pathway increased, while the velocity was enhanced in a non-girdled sector for several days. These findings suggest that lateral sugar transport was enhanced after wounding by a change in the lateral sugar transport path and the axial transport resumed with the development of new conductive tissue.
Please use this url to cite or link to this publication:
author
organization
alternative title
C-11-PET imaging reveals transport dynamics and sectorial plasticity of oak phloem after girdling
year
type
journalArticle (original)
publication status
published
subject
keyword
XYLEM, FLOW, CARBOHYDRATE, PHOTOSYNTHATE, PATTERNS, STEM, TRANSLOCATION, PLANTS, SUGAR-BEET, LONG-DISTANCE TRANSPORT, tracer model, wounding, girdle, assimilates, carbon transport, bark cutting, Quercus robur L., translocation, 11C
journal title
FRONTIERS IN PLANT SCIENCE
Front. Plant Sci.
volume
4
article number
200
pages
9 pages
Web of Science type
Article
Web of Science id
000330166900001
JCR category
PLANT SCIENCES
JCR impact factor
3.637 (2013)
JCR rank
23/199 (2013)
JCR quartile
1 (2013)
ISSN
1664-462X
DOI
10.3389/fpls.2013.00200
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
3607232
handle
http://hdl.handle.net/1854/LU-3607232
date created
2013-06-19 17:03:58
date last changed
2017-03-02 14:12:53
@article{3607232,
  abstract     = {Carbon transport processes in plants can be followed non-invasively by repeated application of the short-lived positron-emitting radioisotope 11C, a technique which has rarely been used with trees. Recently, positron emission tomography (PET) allowing 3D visualisation has been adapted for use with plants. To investigate the effects of stem girdling on the flow of assimilates, leaves on first order branches of two-year-old oak (Quercus robur L.) trees were labelled with 11C by supplying 11CO2-gas to a leaf cuvette. Magnetic resonance imaging gave an indication of the plant structure, while PET registered the tracer flow in a stem region downstream from the labelled branches. After repeated pulse labelling, phloem translocation was shown to be sectorial in the stem: leaf orthostichy determined the position of the phloem sieve tubes containing labelled 11C. The observed pathway remained unchanged for days. Tracer time-series derived from each pulse and analysed with a mechanistic model showed for two adjacent heights in the stem a similar velocity but different loss of recent assimilates. With either complete or partial girdling of bark within the monitored region, transport immediately stopped and then resumed in a new location in the stem cross-section, demonstrating the plasticity of sectoriality. One day after partial girdling, the loss of tracer along the interrupted transport pathway increased, while the velocity was enhanced in a non-girdled sector for several days. These findings suggest that lateral sugar transport was enhanced after wounding by a change in the lateral sugar transport path and the axial transport resumed with the development of new conductive tissue.},
  articleno    = {200},
  author       = {De Schepper, Veerle and B{\"u}hler, Jonas and Thorpe, Michael and Roeb, Gerhard and Huber, Gregor and van Dusschoten, Dagmar and Jahnke, Siegfried and Steppe, Kathy},
  issn         = {1664-462X},
  journal      = {FRONTIERS IN PLANT SCIENCE},
  keyword      = {XYLEM,FLOW,CARBOHYDRATE,PHOTOSYNTHATE,PATTERNS,STEM,TRANSLOCATION,PLANTS,SUGAR-BEET,LONG-DISTANCE TRANSPORT,tracer model,wounding,girdle,assimilates,carbon transport,bark cutting,Quercus robur L.,translocation,11C},
  language     = {eng},
  pages        = {9},
  title        = {11C-PET imaging reveals transport dynamics and sectorial plasticity of oak phloem after girdling},
  url          = {http://dx.doi.org/10.3389/fpls.2013.00200},
  volume       = {4},
  year         = {2013},
}

Chicago
De Schepper, Veerle, Jonas Bühler, Michael Thorpe, Gerhard Roeb, Gregor Huber, Dagmar van Dusschoten, Siegfried Jahnke, and Kathy Steppe. 2013. “11C-PET Imaging Reveals Transport Dynamics and Sectorial Plasticity of Oak Phloem After Girdling.” Frontiers in Plant Science 4.
APA
De Schepper, V., Bühler, J., Thorpe, M., Roeb, G., Huber, G., van Dusschoten, D., Jahnke, S., et al. (2013). 11C-PET imaging reveals transport dynamics and sectorial plasticity of oak phloem after girdling. FRONTIERS IN PLANT SCIENCE, 4.
Vancouver
1.
De Schepper V, Bühler J, Thorpe M, Roeb G, Huber G, van Dusschoten D, et al. 11C-PET imaging reveals transport dynamics and sectorial plasticity of oak phloem after girdling. FRONTIERS IN PLANT SCIENCE. 2013;4.
MLA
De Schepper, Veerle, Jonas Bühler, Michael Thorpe, et al. “11C-PET Imaging Reveals Transport Dynamics and Sectorial Plasticity of Oak Phloem After Girdling.” FRONTIERS IN PLANT SCIENCE 4 (2013): n. pag. Print.