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Proteome-wide characterization of N-glycosylation events by diagonal chromatography

Bart Ghesquière UGent, Jozef Van Damme UGent, Lennart Martens UGent, Joël Vandekerckhove and Kris Gevaert UGent (2006) JOURNAL OF PROTEOME RESEARCH. 5(9). p.2438-2447
abstract
A procedure to map N-glycosylation sites is presented here. It can be applied to purified proteins as well as to highly complex mixtures. The method exploits deglycosylation by PNGase F in a diagonal, reverse-phase chromatographic setup. When applied to 10 mu L of mouse serum, affinity-depleted for its three most abundant components, 117 known or predicted sites were mapped in addition to 10 novel sites. Several sites were detected on soluble membrane or receptor components. Our method furthermore senses the nature of glycan structures and can detect differential glycosylation on a given site. These properties-high sensitivity and dependence on glycan imprinting-can be exploited for glycan-biomarker analysis.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
glycoproteome, diagonal chromatography, COFRADIC, PNGase F, HUMAN ALPHA-1-ACID GLYCOPROTEIN, HAMSTER OVARY CELLS, AMINO-ACID-SEQUENCE, MASS-SPECTROMETRY, TRIFLUOROMETHANESULFONIC ACID, LINKED OLIGOSACCHARIDES, CONGENITAL DISORDERS, HYDRAZIDE CHEMISTRY, CONTAINING PEPTIDES, SOLUBLE FORM
journal title
JOURNAL OF PROTEOME RESEARCH
J. Proteome Res.
volume
5
issue
9
pages
2438 - 2447
Web of Science type
Article
Web of Science id
000240200700042
JCR category
BIOCHEMICAL RESEARCH METHODS
JCR impact factor
5.151 (2006)
JCR rank
7/56 (2006)
JCR quartile
1 (2006)
ISSN
1535-3893
DOI
10.1021/pr060186m
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
356976
handle
http://hdl.handle.net/1854/LU-356976
date created
2007-03-20 09:35:00
date last changed
2016-12-19 15:43:54
@article{356976,
  abstract     = {A procedure to map N-glycosylation sites is presented here. It can be applied to purified proteins as well as to highly complex mixtures. The method exploits deglycosylation by PNGase F in a diagonal, reverse-phase chromatographic setup. When applied to 10 mu L of mouse serum, affinity-depleted for its three most abundant components, 117 known or predicted sites were mapped in addition to 10 novel sites. Several sites were detected on soluble membrane or receptor components. Our method furthermore senses the nature of glycan structures and can detect differential glycosylation on a given site. These properties-high sensitivity and dependence on glycan imprinting-can be exploited for glycan-biomarker analysis.},
  author       = {Ghesqui{\`e}re, Bart and Van Damme, Jozef and Martens, Lennart and Vandekerckhove, Jo{\"e}l and Gevaert, Kris},
  issn         = {1535-3893},
  journal      = {JOURNAL OF PROTEOME RESEARCH},
  keyword      = {glycoproteome,diagonal chromatography,COFRADIC,PNGase F,HUMAN ALPHA-1-ACID GLYCOPROTEIN,HAMSTER OVARY CELLS,AMINO-ACID-SEQUENCE,MASS-SPECTROMETRY,TRIFLUOROMETHANESULFONIC ACID,LINKED OLIGOSACCHARIDES,CONGENITAL DISORDERS,HYDRAZIDE CHEMISTRY,CONTAINING PEPTIDES,SOLUBLE FORM},
  language     = {eng},
  number       = {9},
  pages        = {2438--2447},
  title        = {Proteome-wide characterization of N-glycosylation events by diagonal chromatography},
  url          = {http://dx.doi.org/10.1021/pr060186m},
  volume       = {5},
  year         = {2006},
}

Chicago
Ghesquière, Bart, Jozef Van Damme, Lennart Martens, Joël Vandekerckhove, and Kris Gevaert. 2006. “Proteome-wide Characterization of N-glycosylation Events by Diagonal Chromatography.” Journal of Proteome Research 5 (9): 2438–2447.
APA
Ghesquière, B., Van Damme, J., Martens, L., Vandekerckhove, J., & Gevaert, K. (2006). Proteome-wide characterization of N-glycosylation events by diagonal chromatography. JOURNAL OF PROTEOME RESEARCH, 5(9), 2438–2447.
Vancouver
1.
Ghesquière B, Van Damme J, Martens L, Vandekerckhove J, Gevaert K. Proteome-wide characterization of N-glycosylation events by diagonal chromatography. JOURNAL OF PROTEOME RESEARCH. 2006;5(9):2438–47.
MLA
Ghesquière, Bart, Jozef Van Damme, Lennart Martens, et al. “Proteome-wide Characterization of N-glycosylation Events by Diagonal Chromatography.” JOURNAL OF PROTEOME RESEARCH 5.9 (2006): 2438–2447. Print.