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Porous gelatin hydrogels, 2: in vitro cell interaction study

(2007) BIOMACROMOLECULES. 8(2). p.338-344
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Abstract
We report on the feasibility of applying porous gelatin hydrogels, prepared by a novel and controlled cryogenic treatment, as cell-interactive scaffolds for tissue engineering applications. Despite the large number of publications on gelatin as a biomaterial, a detailed study of screening a limited number of gelatin scaffolds for their interaction with a panel of human cells has, to the best of our knowledge, not yet been published. In the present work, we have evaluated two types of porous gelatin scaffolds that differ in their pore geometry and pore size. Type I hydrogels contained top-to-bottom transverse channels (i.e. cones) with a decreasing diameter from the top (330 mu m) to the bottom (20-30 mu m). Type II hydrogels contained spherical pores with a diameter of 135 mu m. Both types of scaffolds were evaluated by confocal laser scanning microscopy in terms of adhesion, spreading, and proliferation of human cells (endothelial, epithelial, fibroblast, glial, and osteoblast) by visualizing cells using calcein-acetoxy methyl ester as a vital stain. The results indicated that cells attached, spread, and proliferated on both types of hydrogels. In addition, the scaffolds developed can be used for the long-term culturing of human cells.
Keywords
BETA-TRICALCIUM PHOSPHATE, TISSUE ENGINEERING APPLICATIONS, TRI-COPOLYMER SCAFFOLD, HUMAN NUCLEUS PULPOSUS, ENDOTHELIAL-CELLS, CROSS-LINKING, GROWTH-FACTOR, CULTURE, HYDROXYAPATITE, CHONDROCYTES

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Chicago
Dubruel, Peter, R Unger, Sandra Van Vlierberghe, Veerle Cnudde, Patric Jacobs, Etienne Schacht, and CJ Kirkpatrick. 2007. “Porous Gelatin Hydrogels, 2: In Vitro Cell Interaction Study.” Biomacromolecules 8 (2): 338–344.
APA
Dubruel, P., Unger, R., Van Vlierberghe, S., Cnudde, V., Jacobs, P., Schacht, E., & Kirkpatrick, C. (2007). Porous gelatin hydrogels, 2: in vitro cell interaction study. BIOMACROMOLECULES, 8(2), 338–344.
Vancouver
1.
Dubruel P, Unger R, Van Vlierberghe S, Cnudde V, Jacobs P, Schacht E, et al. Porous gelatin hydrogels, 2: in vitro cell interaction study. BIOMACROMOLECULES. 2007;8(2):338–44.
MLA
Dubruel, Peter, R Unger, Sandra Van Vlierberghe, et al. “Porous Gelatin Hydrogels, 2: In Vitro Cell Interaction Study.” BIOMACROMOLECULES 8.2 (2007): 338–344. Print.
@article{356363,
  abstract     = {We report on the feasibility of applying porous gelatin hydrogels, prepared by a novel and controlled cryogenic treatment, as cell-interactive scaffolds for tissue engineering applications. Despite the large number of publications on gelatin as a biomaterial, a detailed study of screening a limited number of gelatin scaffolds for their interaction with a panel of human cells has, to the best of our knowledge, not yet been published. In the present work, we have evaluated two types of porous gelatin scaffolds that differ in their pore geometry and pore size. Type I hydrogels contained top-to-bottom transverse channels (i.e. cones) with a decreasing diameter from the top (330 mu m) to the bottom (20-30 mu m). Type II hydrogels contained spherical pores with a diameter of 135 mu m. Both types of scaffolds were evaluated by confocal laser scanning microscopy in terms of adhesion, spreading, and proliferation of human cells (endothelial, epithelial, fibroblast, glial, and osteoblast) by visualizing cells using calcein-acetoxy methyl ester as a vital stain. The results indicated that cells attached, spread, and proliferated on both types of hydrogels. In addition, the scaffolds developed can be used for the long-term culturing of human cells.},
  author       = {Dubruel, Peter and Unger, R and Van Vlierberghe, Sandra and Cnudde, Veerle and Jacobs, Patric and Schacht, Etienne and Kirkpatrick, CJ},
  issn         = {1525-7797},
  journal      = {BIOMACROMOLECULES},
  keyword      = {BETA-TRICALCIUM PHOSPHATE,TISSUE ENGINEERING APPLICATIONS,TRI-COPOLYMER SCAFFOLD,HUMAN NUCLEUS PULPOSUS,ENDOTHELIAL-CELLS,CROSS-LINKING,GROWTH-FACTOR,CULTURE,HYDROXYAPATITE,CHONDROCYTES},
  language     = {eng},
  number       = {2},
  pages        = {338--344},
  title        = {Porous gelatin hydrogels, 2: in vitro cell interaction study},
  url          = {http://dx.doi.org/10.1021/bm0606869},
  volume       = {8},
  year         = {2007},
}

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