Increased angiogenesis and permeability in the mesenteric microvasculature of rats with cirrhosis and portal hypertension: an in vivo study
- Author
- Anja Geerts (UGent) , An De Vriese (UGent) , Eline Vanheule (UGent) , Hans Van Vlierberghe (UGent) , Siska Mortier, Kin Jip Cheung (UGent) , Pieter Demetter, Norbert Lameire (UGent) , Martine De Vos (UGent) and Isabelle Colle (UGent)
- Organization
- Abstract
- Background: In vivo evidence for angiogenesis in the splanchnic vasodilation in portal hypertension (PHT) and cirrhosis is lacking. Vascular endothelial growth factor (VEGF) and endothelial nitric oxide synthase (eNOS) are mediators of angiogenesis. The present study visualises in vivo structural changes (angiogenesis and vascular hyperpermeability) and examines the presence of VEGF and eNOS in the mesenteric microvasculature of animal models of PHT with and without cirrhosis. Methods: Portal hypertension was induced by partial portal vein ligation (PPVL) and cirrhosis was induced by common bile duct ligation (CBDL) in rats. The mesenteric microcirculation was examined by intravital microscopy. Expression of VEGF, eNOS and CD31 in mesenteric tissue were studied by immunohistochemistry. Results: An increased mesenteric angiogenesis was observed in PPVL and CBDL rats compared with Sham-operated and control rats, as shown by intravital microscopy and CD 31 staining. VEGF and eNOS expression was higher in CBDL and PPVL rats compared with control groups and correlated positively with vascular density. Macromolecular leakage was increased in cirrhotic rats compared with control and PPVL rats. Conclusion: Our study provides in vivo evidence of an increased angiogenesis in the mesenteric microvasculature of animal models of PHT and cirrhosis. Increased VEGF and eNOS expression in the mesentery of PPVL and CBDL rats may suggest their contribution. Microvascular permeability in the mesenteric vessels was only increased in cirrhotic rats.
- Keywords
- ENDOTHELIAL GROWTH-FACTOR, angiogenesis, NITRIC-OXIDE SYNTHASE, PERITONEAL-MACROPHAGES, VASCULAR-PERMEABILITY, BILIARY-CIRRHOSIS, VEGF, MICE, CIRCULATION, INHIBITION, MEMBRANE, vascular endothelial growth factor, cirrhosis, endothelial nitric oxide synthase, intravital microscopy, portal hypertension
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-354553
- MLA
- Geerts, Anja, et al. “Increased Angiogenesis and Permeability in the Mesenteric Microvasculature of Rats with Cirrhosis and Portal Hypertension: An in Vivo Study.” LIVER INTERNATIONAL, vol. 26, no. 7, 2006, pp. 889–98, doi:10.1111/j.1478-3231.2006.01308.x.
- APA
- Geerts, A., De Vriese, A., Vanheule, E., Van Vlierberghe, H., Mortier, S., Cheung, K. J., … Colle, I. (2006). Increased angiogenesis and permeability in the mesenteric microvasculature of rats with cirrhosis and portal hypertension: an in vivo study. LIVER INTERNATIONAL, 26(7), 889–898. https://doi.org/10.1111/j.1478-3231.2006.01308.x
- Chicago author-date
- Geerts, Anja, An De Vriese, Eline Vanheule, Hans Van Vlierberghe, Siska Mortier, Kin Jip Cheung, Pieter Demetter, Norbert Lameire, Martine De Vos, and Isabelle Colle. 2006. “Increased Angiogenesis and Permeability in the Mesenteric Microvasculature of Rats with Cirrhosis and Portal Hypertension: An in Vivo Study.” LIVER INTERNATIONAL 26 (7): 889–98. https://doi.org/10.1111/j.1478-3231.2006.01308.x.
- Chicago author-date (all authors)
- Geerts, Anja, An De Vriese, Eline Vanheule, Hans Van Vlierberghe, Siska Mortier, Kin Jip Cheung, Pieter Demetter, Norbert Lameire, Martine De Vos, and Isabelle Colle. 2006. “Increased Angiogenesis and Permeability in the Mesenteric Microvasculature of Rats with Cirrhosis and Portal Hypertension: An in Vivo Study.” LIVER INTERNATIONAL 26 (7): 889–898. doi:10.1111/j.1478-3231.2006.01308.x.
- Vancouver
- 1.Geerts A, De Vriese A, Vanheule E, Van Vlierberghe H, Mortier S, Cheung KJ, et al. Increased angiogenesis and permeability in the mesenteric microvasculature of rats with cirrhosis and portal hypertension: an in vivo study. LIVER INTERNATIONAL. 2006;26(7):889–98.
- IEEE
- [1]A. Geerts et al., “Increased angiogenesis and permeability in the mesenteric microvasculature of rats with cirrhosis and portal hypertension: an in vivo study,” LIVER INTERNATIONAL, vol. 26, no. 7, pp. 889–898, 2006.
@article{354553, abstract = {{Background: In vivo evidence for angiogenesis in the splanchnic vasodilation in portal hypertension (PHT) and cirrhosis is lacking. Vascular endothelial growth factor (VEGF) and endothelial nitric oxide synthase (eNOS) are mediators of angiogenesis. The present study visualises in vivo structural changes (angiogenesis and vascular hyperpermeability) and examines the presence of VEGF and eNOS in the mesenteric microvasculature of animal models of PHT with and without cirrhosis. Methods: Portal hypertension was induced by partial portal vein ligation (PPVL) and cirrhosis was induced by common bile duct ligation (CBDL) in rats. The mesenteric microcirculation was examined by intravital microscopy. Expression of VEGF, eNOS and CD31 in mesenteric tissue were studied by immunohistochemistry. Results: An increased mesenteric angiogenesis was observed in PPVL and CBDL rats compared with Sham-operated and control rats, as shown by intravital microscopy and CD 31 staining. VEGF and eNOS expression was higher in CBDL and PPVL rats compared with control groups and correlated positively with vascular density. Macromolecular leakage was increased in cirrhotic rats compared with control and PPVL rats. Conclusion: Our study provides in vivo evidence of an increased angiogenesis in the mesenteric microvasculature of animal models of PHT and cirrhosis. Increased VEGF and eNOS expression in the mesentery of PPVL and CBDL rats may suggest their contribution. Microvascular permeability in the mesenteric vessels was only increased in cirrhotic rats.}}, author = {{Geerts, Anja and De Vriese, An and Vanheule, Eline and Van Vlierberghe, Hans and Mortier, Siska and Cheung, Kin Jip and Demetter, Pieter and Lameire, Norbert and De Vos, Martine and Colle, Isabelle}}, issn = {{1478-3223}}, journal = {{LIVER INTERNATIONAL}}, keywords = {{ENDOTHELIAL GROWTH-FACTOR,angiogenesis,NITRIC-OXIDE SYNTHASE,PERITONEAL-MACROPHAGES,VASCULAR-PERMEABILITY,BILIARY-CIRRHOSIS,VEGF,MICE,CIRCULATION,INHIBITION,MEMBRANE,vascular endothelial growth factor,cirrhosis,endothelial nitric oxide synthase,intravital microscopy,portal hypertension}}, language = {{eng}}, number = {{7}}, pages = {{889--898}}, title = {{Increased angiogenesis and permeability in the mesenteric microvasculature of rats with cirrhosis and portal hypertension: an in vivo study}}, url = {{http://doi.org/10.1111/j.1478-3231.2006.01308.x}}, volume = {{26}}, year = {{2006}}, }
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