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T-, B- and NK-lymphoid, but not myeloid cells arise from human CD34+CD38-CD7+ common lymphoid progenitors expressing lymphoid-specific genes

(2007) LEUKEMIA. 21(2). p.311-319
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Abstract
Hematopoietic stem cells in the bone marrow ( BM) give rise to all blood cells. According to the classic model of hematopoiesis, the differentiation paths leading to the myeloid and lymphoid lineages segregate early. A candidate 'common lymphoid progenitor' (CLP) has been isolated from CD34(+)CD38(-) human cord blood cells based on CD7 expression. Here, we confirm the B- and NK-differentiation potential of CD34(+)CD38(-)CD7(-) cells and show in addition that this population has strong capacity to differentiate into T cells. As CD34(+)CD38(-)CD7(+) cells are virtually devoid of myeloid differentiation potential, these cells represent true CLPs. To unravel the molecular mechanisms underlying lymphoid commitment, we performed genome-wide gene expression profiling on sorted CD34(+)CD38(-)CD7(+) and CD34(+)CD38(-)CD7(+) cells. Interestingly, lymphoid-affiliated genes were mainly upregulated in the CD7(+) population, while myeloid-specific genes were downregulated. This supports the hypothesis that lineage commitment is accompanied by the shutdown of inappropriate gene expression and the upregulation of lineage-specific genes. In addition, we identified several highly expressed genes that have not been described in hematopoiesis before.
Keywords
cord blood, human, hematopoiesis, stem cells, lymphoid progenitor, BONE-MARROW, CORD BLOOD, IDENTIFICATION, CYTOSKELETON, LINEAGES, DIFFERENTIATION, REARRANGEMENT, COMMITMENT, RECEPTOR, TISSUES

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Chicago
Hoebeke, Inge, Magda De Smedt, Frank Stolz, K Pike-Overzet, FJT Staal, Jean Plum, and Georges Leclercq. 2007. “T-, B- and NK-lymphoid, but Not Myeloid Cells Arise from Human CD34+CD38-CD7+ Common Lymphoid Progenitors Expressing Lymphoid-specific Genes.” Leukemia 21 (2): 311–319.
APA
Hoebeke, I., De Smedt, M., Stolz, F., Pike-Overzet, K., Staal, F., Plum, J., & Leclercq, G. (2007). T-, B- and NK-lymphoid, but not myeloid cells arise from human CD34+CD38-CD7+ common lymphoid progenitors expressing lymphoid-specific genes. LEUKEMIA, 21(2), 311–319.
Vancouver
1.
Hoebeke I, De Smedt M, Stolz F, Pike-Overzet K, Staal F, Plum J, et al. T-, B- and NK-lymphoid, but not myeloid cells arise from human CD34+CD38-CD7+ common lymphoid progenitors expressing lymphoid-specific genes. LEUKEMIA. 2007;21(2):311–9.
MLA
Hoebeke, Inge, Magda De Smedt, Frank Stolz, et al. “T-, B- and NK-lymphoid, but Not Myeloid Cells Arise from Human CD34+CD38-CD7+ Common Lymphoid Progenitors Expressing Lymphoid-specific Genes.” LEUKEMIA 21.2 (2007): 311–319. Print.
@article{354433,
  abstract     = {Hematopoietic stem cells in the bone marrow ( BM) give rise to all blood cells. According to the classic model of hematopoiesis, the differentiation paths leading to the myeloid and lymphoid lineages segregate early. A candidate 'common lymphoid progenitor' (CLP) has been isolated from CD34(+)CD38(-) human cord blood cells based on CD7 expression. Here, we confirm the B- and NK-differentiation potential of CD34(+)CD38(-)CD7(-) cells and show in addition that this population has strong capacity to differentiate into T cells. As CD34(+)CD38(-)CD7(+) cells are virtually devoid of myeloid differentiation potential, these cells represent true CLPs. To unravel the molecular mechanisms underlying lymphoid commitment, we performed genome-wide gene expression profiling on sorted CD34(+)CD38(-)CD7(+) and CD34(+)CD38(-)CD7(+) cells. Interestingly, lymphoid-affiliated genes were mainly upregulated in the CD7(+) population, while myeloid-specific genes were downregulated. This supports the hypothesis that lineage commitment is accompanied by the shutdown of inappropriate gene expression and the upregulation of lineage-specific genes. In addition, we identified several highly expressed genes that have not been described in hematopoiesis before.},
  author       = {Hoebeke, Inge and De Smedt, Magda and Stolz, Frank and Pike-Overzet, K and Staal, FJT and Plum, Jean and Leclercq, Georges},
  issn         = {0887-6924},
  journal      = {LEUKEMIA},
  language     = {eng},
  number       = {2},
  pages        = {311--319},
  title        = {T-, B- and NK-lymphoid, but not myeloid cells arise from human CD34+CD38-CD7+ common lymphoid progenitors expressing lymphoid-specific genes},
  url          = {http://dx.doi.org/10.1038/sj.leu.2404488},
  volume       = {21},
  year         = {2007},
}

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