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Taeniasis-cysticercosis in Southern Ecuador : assessment of infection status using multiple laboratory diagnostic tools

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Abstract
Taenia solium-taeniasis and cysticercosis were studied in the human and porcine populations of a rural community in the Southern Ecuadorian Andes. From the 1059 inhabitants, 800 serum samples and 958 stool samples could he collected. In addition, 646 from the estimated 1148 pigs were tongue inspected. Circulating antigen was detected by enzyme linked immunosorbent assay (Ag-ELISA) in 2.25% of the human population, whereas intestinal taeniasis was detected in 1.46% by the formalin-ether technique. Following treatment and recovery of tapeworm fragments these were all identified as T. solium. Porcine cysticercosis was diagnosed in 3.56% of the pigs by tongue inspection. In addition, enzyme linked immunoelectrotransfer blot (EITB) was performed on a subset group of 100 humans to confirm the results of the Ag-ELISA. One hundred serum samples from pigs were also analysed by EITB. It appeared that 43 and 74% of humans and pigs had antibodies against T. solium cysticerci, respectively. It is concluded that contrary to the high exposure of the human population to T. solium. that is suggested by EITB, the number of active cysticercosis cases, diagnosed by Ag-ELISA, was low, which may indicate endemic stability. The further use of complementary diagnostic methods for a better understanding of the epidemiology of T. solium. is suggested.
Keywords
restricted fragment length polymerase, Taenia solium, Ecuador, PORCINE CYSTICERCOSIS, TAENIOSIS-CYSTICERCOSIS, SAGINATA CYSTICERCOSIS, SOLIUM, VILLAGE, EPIDEMIOLOGY, PREVALENCE, ANTIGENS, ELISA, PERU, polymerase chain reaction, taeniasis, cysticercosis, enzyme linked immunosorbent assay, enzyme-linked immunoelectrotransfer blot

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Please use this url to cite or link to this publication:

Chicago
Rodriguez-Hidalgo, R, W Benitez-Ortiz, N Praet, LR Saa, Jozef Vercruysse, J Brandt, and Pierre Dorny. 2006. “Taeniasis-cysticercosis in Southern Ecuador : Assessment of Infection Status Using Multiple Laboratory Diagnostic Tools.” Memorias Do Instituto Oswaldo Cruz 101 (7): 779–782.
APA
Rodriguez-Hidalgo, R., Benitez-Ortiz, W., Praet, N., Saa, L., Vercruysse, J., Brandt, J., & Dorny, P. (2006). Taeniasis-cysticercosis in Southern Ecuador : assessment of infection status using multiple laboratory diagnostic tools. MEMORIAS DO INSTITUTO OSWALDO CRUZ, 101(7), 779–782.
Vancouver
1.
Rodriguez-Hidalgo R, Benitez-Ortiz W, Praet N, Saa L, Vercruysse J, Brandt J, et al. Taeniasis-cysticercosis in Southern Ecuador : assessment of infection status using multiple laboratory diagnostic tools. MEMORIAS DO INSTITUTO OSWALDO CRUZ. 2006;101(7):779–82.
MLA
Rodriguez-Hidalgo, R, W Benitez-Ortiz, N Praet, et al. “Taeniasis-cysticercosis in Southern Ecuador : Assessment of Infection Status Using Multiple Laboratory Diagnostic Tools.” MEMORIAS DO INSTITUTO OSWALDO CRUZ 101.7 (2006): 779–782. Print.
@article{354372,
  abstract     = {Taenia solium-taeniasis and cysticercosis were studied in the human and porcine populations of a rural community in the Southern Ecuadorian Andes. From the 1059 inhabitants, 800 serum samples and 958 stool samples could he collected. In addition, 646 from the estimated 1148 pigs were tongue inspected. Circulating antigen was detected by enzyme linked immunosorbent assay (Ag-ELISA) in 2.25\% of the human population, whereas intestinal taeniasis was detected in 1.46\% by the formalin-ether technique. Following treatment and recovery of tapeworm fragments these were all identified as T. solium. Porcine cysticercosis was diagnosed in 3.56\% of the pigs by tongue inspection. 
In addition, enzyme linked immunoelectrotransfer blot (EITB) was performed on a subset group of 100 humans to confirm the results of the Ag-ELISA. One hundred serum samples from pigs were also analysed by EITB. It appeared that 43 and 74\% of humans and pigs had antibodies against T. solium cysticerci, respectively. It is concluded that contrary to the high exposure of the human population to T. solium. that is suggested by EITB, the number of active cysticercosis cases, diagnosed by Ag-ELISA, was low, which may indicate endemic stability. The further use of complementary diagnostic methods for a better understanding of the epidemiology of T. solium. is suggested.},
  author       = {Rodriguez-Hidalgo, R and Benitez-Ortiz, W and Praet, N and Saa, LR and Vercruysse, Jozef and Brandt, J and Dorny, Pierre},
  issn         = {0074-0276},
  journal      = {MEMORIAS DO INSTITUTO OSWALDO CRUZ},
  language     = {eng},
  number       = {7},
  pages        = {779--782},
  title        = {Taeniasis-cysticercosis in Southern Ecuador : assessment of infection status using multiple laboratory diagnostic tools},
  url          = {http://memorias.ioc.fiocruz.br/101(7)/5623.html},
  volume       = {101},
  year         = {2006},
}

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