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The predominance of Human Immunodeficiency Virus type 1 (HIV-1) circulating recombinant form 02 (CRF02_AG) in West Central Africa may be related to its replicative fitness

Harr F Njai, Youssef Gali, Guido Vanham, Claude Clybergh, Wim Jennes, Nicole Vidal, Christelle Butel, Eitel Mpoudi-Ngolle, Martine Peeters and Kevin Ariën UGent (2006) RETROVIROLOGY. 3.
abstract
Background: CRF02_AG is the predominant HIV strain circulating in West and West Central Africa. The aim of this study was to test whether this predominance is associated with a higher in vitro replicative fitness relative to parental subtype A and G viruses. Primary HIV-I isolates (10 CRF02_AG, 5 subtype A and 5 subtype G) were obtained from a well-described Cameroonian cohort. Growth competition experiments were carried out at equal multiplicity of infection in activated T cells and monocyte-derived dendritic cells (MO-DC) in parallel. Results: Dual infection/ competition experiments in activated T cells clearly indicated that CRF02_AG isolates had a significant replication advantage over the subtype A and subtype G viruses. The higher fitness of CRF02_AG was evident for isolates from patients with CD4+ T cell counts > 200 cells/mu L (non-AIDS) or CD4+ T cell counts < 200 cells/mu L (AIDS), and was independent of the co-receptor tropism. In MO-DC cultures, CRF02_AG isolates showed a slightly but not significantly higher replication advantage compared to subtype A or G isolates. Conclusion: We observed a higher ex vivo replicative fitness of CRF02_AG isolates compared to subtype A and G viruses from the same geographic region and showed that this was independent of the co-receptor tropism and irrespective of high or low CD4+ T cell count. This advantage in replicative fitness may contribute to the dominant spread of CRF02_AG over A and G subtypes in West and West Central Africa.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
DISEASE PROGRESSION, MOLECULAR EPIDEMIOLOGY, GROUP-O, INFECTION, CAMEROON, INTRAVAGINAL INOCULATION, T-CELLS, SUBTYPE-G, GENETIC DIVERSITY, DENDRITIC CELLS
journal title
RETROVIROLOGY
Retrovirology
volume
3
article_number
40
pages
11 pages
Web of Science type
Article
Web of Science id
000239505100001
ISSN
1742-4690
DOI
10.1186/1742-4690-3-40
language
English
UGent publication?
no
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
352686
handle
http://hdl.handle.net/1854/LU-352686
date created
2007-01-17 13:40:00
date last changed
2010-11-16 15:10:48
@article{352686,
  abstract     = {Background: CRF02\_AG is the predominant HIV strain circulating in West and West Central Africa. The aim of this study was to test whether this predominance is associated with a higher in vitro replicative fitness relative to parental subtype A and G viruses. Primary HIV-I isolates (10 CRF02\_AG, 5 subtype A and 5 subtype G) were obtained from a well-described Cameroonian cohort. Growth competition experiments were carried out at equal multiplicity of infection in activated T cells and monocyte-derived dendritic cells (MO-DC) in parallel.
Results: Dual infection/ competition experiments in activated T cells clearly indicated that CRF02\_AG isolates had a significant replication advantage over the subtype A and subtype G viruses. The higher fitness of CRF02\_AG was evident for isolates from patients with CD4+ T cell counts {\textrangle} 200 cells/mu L (non-AIDS) or CD4+ T cell counts {\textlangle} 200 cells/mu L (AIDS), and was independent of the co-receptor tropism. In MO-DC cultures, CRF02\_AG isolates showed a slightly but not significantly higher replication advantage compared to subtype A or G isolates.
Conclusion: We observed a higher ex vivo replicative fitness of CRF02\_AG isolates compared to subtype A and G viruses from the same geographic region and showed that this was independent of the co-receptor tropism and irrespective of high or low CD4+ T cell count. This advantage in replicative fitness may contribute to the dominant spread of CRF02\_AG over A and G subtypes in West and West Central Africa.},
  articleno    = {40},
  author       = {Njai, Harr F and Gali, Youssef and Vanham, Guido and Clybergh, Claude and Jennes, Wim and Vidal, Nicole and Butel, Christelle and Mpoudi-Ngolle, Eitel and Peeters, Martine and Ari{\"e}n, Kevin},
  issn         = {1742-4690},
  journal      = {RETROVIROLOGY},
  keyword      = {DISEASE PROGRESSION,MOLECULAR EPIDEMIOLOGY,GROUP-O,INFECTION,CAMEROON,INTRAVAGINAL INOCULATION,T-CELLS,SUBTYPE-G,GENETIC DIVERSITY,DENDRITIC CELLS},
  language     = {eng},
  pages        = {11},
  title        = {The predominance of Human Immunodeficiency Virus type 1 (HIV-1) circulating recombinant form 02 (CRF02\_AG) in West Central Africa may be related to its replicative fitness},
  url          = {http://dx.doi.org/10.1186/1742-4690-3-40},
  volume       = {3},
  year         = {2006},
}

Chicago
Njai, Harr F, Youssef Gali, Guido Vanham, Claude Clybergh, Wim Jennes, Nicole Vidal, Christelle Butel, Eitel Mpoudi-Ngolle, Martine Peeters, and Kevin Ariën. 2006. “The Predominance of Human Immunodeficiency Virus Type 1 (HIV-1) Circulating Recombinant Form 02 (CRF02_AG) in West Central Africa May Be Related to Its Replicative Fitness.” Retrovirology 3.
APA
Njai, H. F., Gali, Y., Vanham, G., Clybergh, C., Jennes, W., Vidal, N., Butel, C., et al. (2006). The predominance of Human Immunodeficiency Virus type 1 (HIV-1) circulating recombinant form 02 (CRF02_AG) in West Central Africa may be related to its replicative fitness. RETROVIROLOGY, 3.
Vancouver
1.
Njai HF, Gali Y, Vanham G, Clybergh C, Jennes W, Vidal N, et al. The predominance of Human Immunodeficiency Virus type 1 (HIV-1) circulating recombinant form 02 (CRF02_AG) in West Central Africa may be related to its replicative fitness. RETROVIROLOGY. 2006;3.
MLA
Njai, Harr F, Youssef Gali, Guido Vanham, et al. “The Predominance of Human Immunodeficiency Virus Type 1 (HIV-1) Circulating Recombinant Form 02 (CRF02_AG) in West Central Africa May Be Related to Its Replicative Fitness.” RETROVIROLOGY 3 (2006): n. pag. Print.