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Effect of carprofen treatment following experimentally induced Escherichia coli mastitis in primiparous cows

(2005) JOURNAL OF DAIRY SCIENCE. 88(7). p.2361-2376
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Abstract
Acute Escherichia coli mastitis is one of the major sources of economic loss in the dairy industry due to reduced milk production, treatment costs, discarded milk, and occasional fatal disease. Nonsteroidal anti-inflammatory drugs ( NSAIDs) are frequently used as adjunctive therapy to antibiotics. The objective of the current study was to evaluate the effect of carprofen treatment following infusion of Escherichia coli into the mammary glands of primiparous cows during the periparturient period. Severity of mastitis was scored based on the average milk production in the uninfected quarters on d+2 postinoculation and a clinical severity score. Carprofen was administered intravenously at 9 h postchallenge, when clinical signs of mastitis appeared. In previous work, efficacy of NSAIDs was mainly evaluated using clinical symptoms. In the present study, the effect of carprofen on innate immune response was also assessed by quantification of inflammatory mediators. All primiparous cows reacted as moderate responders throughout the experimental period. Primiparous cows were intramammarily inoculated with 1x10(4) cfu of E. coli P4:O32 in 2 left quarters. Analysis of blood and milk parameters, including IL-8, complement component C5a, lipopolysaccharide-binding protein (LBP), soluble CD14, prostaglandin E-2, and thromboxane B-2 was performed from d0 to d+6 relative to intramammary inoculation. Rectal temperature in carprofen-treated animals was lower than in control animals at 3 and 6 h posttreatment. Treatment also restored the decreased reticulorumen motility that occurs during E. coli mastitis to preinfection levels faster than in control animals. Carprofen treatment resulted in an earlier normalization of the clinical severity score. Eicosanoid (prostaglandin E-2 and thromboxane B-2) production in milk tended to be inhibited by carprofen. No significant differences in the kinetic patterns of somatic cell count, IL-8, complement component C5a, LBP, and soluble CD14 were observed. In conclusion, carprofen treatment improved general clinical condition by effective antipyrexia and restoration of reticulorumen motility but did not significantly inhibit eicosanoid production. Carprofen treatment did not result in a significant decrease of chemotactic inflammatory mediators, IL-8 and C5a, and early innate immune molecules, sCD14 and LBP. Therefore, major modulatory effects from NSAID administration were not observed in this mastitis model, although a larger study might confirm some apparent trends obtained in the present results.
Keywords
LACTATING GOATS, SOLUBLE CD14, DAIRY-COWS, NONSTEROIDAL ANTIINFLAMMATORY DRUGS, ARACHIDONIC-ACID METABOLITES, MAMMARY BLOOD-FLOW, CD14 REDUCES SEVERITY, FLUNIXIN MEGLUMINE, carprofen, primiparous cow, Escherichia coli mastitis, ENDOTOXIN-INDUCED MASTITIS, INDUCED BOVINE MASTITIS, moderate inflammation

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MLA
Vangroenweghe, Frédéric, et al. “Effect of Carprofen Treatment Following Experimentally Induced Escherichia Coli Mastitis in Primiparous Cows.” JOURNAL OF DAIRY SCIENCE, vol. 88, no. 7, 2005, pp. 2361–76.
APA
Vangroenweghe, F., Duchateau, L., Boutet, P., Lekeux, P., Rainard, P., Paape, M., & Burvenich, C. (2005). Effect of carprofen treatment following experimentally induced Escherichia coli mastitis in primiparous cows. JOURNAL OF DAIRY SCIENCE, 88(7), 2361–2376.
Chicago author-date
Vangroenweghe, Frédéric, Luc Duchateau, P Boutet, P Lekeux, P Rainard, Max Paape, and Christian Burvenich. 2005. “Effect of Carprofen Treatment Following Experimentally Induced Escherichia Coli Mastitis in Primiparous Cows.” JOURNAL OF DAIRY SCIENCE 88 (7): 2361–76.
Chicago author-date (all authors)
Vangroenweghe, Frédéric, Luc Duchateau, P Boutet, P Lekeux, P Rainard, Max Paape, and Christian Burvenich. 2005. “Effect of Carprofen Treatment Following Experimentally Induced Escherichia Coli Mastitis in Primiparous Cows.” JOURNAL OF DAIRY SCIENCE 88 (7): 2361–2376.
Vancouver
1.
Vangroenweghe F, Duchateau L, Boutet P, Lekeux P, Rainard P, Paape M, et al. Effect of carprofen treatment following experimentally induced Escherichia coli mastitis in primiparous cows. JOURNAL OF DAIRY SCIENCE. 2005;88(7):2361–76.
IEEE
[1]
F. Vangroenweghe et al., “Effect of carprofen treatment following experimentally induced Escherichia coli mastitis in primiparous cows,” JOURNAL OF DAIRY SCIENCE, vol. 88, no. 7, pp. 2361–2376, 2005.
@article{350235,
  abstract     = {{Acute Escherichia coli mastitis is one of the major sources of economic loss in the dairy industry due to reduced milk production, treatment costs, discarded milk, and occasional fatal disease. Nonsteroidal anti-inflammatory drugs ( NSAIDs) are frequently used as adjunctive therapy to antibiotics. The objective of the current study was to evaluate the effect of carprofen treatment following infusion of Escherichia coli into the mammary glands of primiparous cows during the periparturient period. Severity of mastitis was scored based on the average milk production in the uninfected quarters on d+2 postinoculation and a clinical severity score. Carprofen was administered intravenously at 9 h postchallenge, when clinical signs of mastitis appeared. In previous work, efficacy of NSAIDs was mainly evaluated using clinical symptoms. In the present study, the effect of carprofen on innate immune response was also assessed by quantification of inflammatory mediators. All primiparous cows reacted as moderate responders throughout the experimental period. Primiparous cows were intramammarily inoculated with 1x10(4) cfu of E. coli P4:O32 in 2 left quarters. Analysis of blood and milk parameters, including IL-8, complement component C5a, lipopolysaccharide-binding protein (LBP), soluble CD14, prostaglandin E-2, and thromboxane B-2 was performed from d0 to d+6 relative to intramammary inoculation. Rectal temperature in carprofen-treated animals was lower than in control animals at 3 and 6 h posttreatment. Treatment also restored the decreased reticulorumen motility that occurs during E. coli mastitis to preinfection levels faster than in control animals. Carprofen treatment resulted in an earlier normalization of the clinical severity score. Eicosanoid (prostaglandin E-2 and thromboxane B-2) production in milk tended to be inhibited by carprofen. No significant differences in the kinetic patterns of somatic cell count, IL-8, complement component C5a, LBP, and soluble CD14 were observed. In conclusion, carprofen treatment improved general clinical condition by effective antipyrexia and restoration of reticulorumen motility but did not significantly inhibit eicosanoid production. Carprofen treatment did not result in a significant decrease of chemotactic inflammatory mediators, IL-8 and C5a, and early innate immune molecules, sCD14 and LBP. Therefore, major modulatory effects from NSAID administration were not observed in this mastitis model, although a larger study might confirm some apparent trends obtained in the present results.}},
  author       = {{Vangroenweghe, Frédéric and Duchateau, Luc and Boutet, P and Lekeux, P and Rainard, P and Paape, Max and Burvenich, Christian}},
  issn         = {{0022-0302}},
  journal      = {{JOURNAL OF DAIRY SCIENCE}},
  keywords     = {{LACTATING GOATS,SOLUBLE CD14,DAIRY-COWS,NONSTEROIDAL ANTIINFLAMMATORY DRUGS,ARACHIDONIC-ACID METABOLITES,MAMMARY BLOOD-FLOW,CD14 REDUCES SEVERITY,FLUNIXIN MEGLUMINE,carprofen,primiparous cow,Escherichia coli mastitis,ENDOTOXIN-INDUCED MASTITIS,INDUCED BOVINE MASTITIS,moderate inflammation}},
  language     = {{eng}},
  number       = {{7}},
  pages        = {{2361--2376}},
  title        = {{Effect of carprofen treatment following experimentally induced Escherichia coli mastitis in primiparous cows}},
  url          = {{http://jds.fass.org/cgi/content/abstract/88/7/2361}},
  volume       = {{88}},
  year         = {{2005}},
}

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