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Overexpression of HES-1 is not sufficient to impose T-cell differentiation on human hernatopoietic stem cells

Inge Hoebeke UGent, Magda De Smedt UGent, Inge Van de Walle UGent, Katia Reynvoet UGent, Greet De Smet UGent, Jean Plum UGent and Georges Leclercq UGent (2006) BLOOD. 107(7). p.2879-2881
abstract
By retroviral overexpression of the Notch-1 intracellular domain (ICN) in human CD34(+) hematopoietic stem cells (HSCs), we have shown previously that Notch-1 signaling promotes the T-cell fate and inhibits the monocyte and B-cell fate in several in vitro and in vivo differentiation assays. Here, we Investigated whether the effects of constitutively active Notch-1 can be mimicked by overexpression of its downstream target gene HES1. Upon HES-1 retroviral transduction, human CD34+ stem cells had a different outcome in the differentiation assays as compared to ICN-transduced cells. Although HES-1 induced a partial block in B-cell development, it did not inhibit monocyte development and did not promote T/NK-cell-lineage differentiation. On the contrary, a higher percentage of HES-1-transduced stem cells remained CD34+. These experiments indicate that HES-1 alone is not able to substitute for Notch-1 signaling to induce T-cell differentiation of human CD34(+) hematopoietic stem cells.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
THYMUS, NOTCH SIGNALING PATHWAY, MICE, LINEAGE, MARROW, DELTA-1, PRECURSORS, EXPRESSION, IN-VIVO, FATE
journal title
BLOOD
Blood
volume
107
issue
7
pages
2879 - 2881
Web of Science type
Article
Web of Science id
000236656900051
JCR category
HEMATOLOGY
JCR impact factor
10.37 (2006)
JCR rank
2/61 (2006)
JCR quartile
1 (2006)
ISSN
0006-4971
DOI
10.1182/blood-2005-05-1815
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
338358
handle
http://hdl.handle.net/1854/LU-338358
date created
2006-06-07 17:03:00
date last changed
2011-05-27 12:49:40
@article{338358,
  abstract     = {By retroviral overexpression of the Notch-1 intracellular domain (ICN) in human CD34(+) hematopoietic stem cells (HSCs), we have shown previously that Notch-1 signaling promotes the T-cell fate and inhibits the monocyte and B-cell fate in several in vitro and in vivo differentiation assays. Here, we Investigated whether the effects of constitutively active Notch-1 can be mimicked by overexpression of its downstream target gene HES1. Upon HES-1 retroviral transduction, human CD34+ stem cells had a different outcome in the differentiation assays as compared to ICN-transduced cells. Although HES-1 induced a partial block in B-cell development, it did not inhibit monocyte development and did not promote T/NK-cell-lineage differentiation. On the contrary, a higher percentage of HES-1-transduced stem cells remained CD34+. These experiments indicate that HES-1 alone is not able to substitute for Notch-1 signaling to induce T-cell differentiation of human CD34(+) hematopoietic stem cells.},
  author       = {Hoebeke, Inge and De Smedt, Magda and Van de Walle, Inge and Reynvoet, Katia and De Smet, Greet and Plum, Jean and Leclercq, Georges},
  issn         = {0006-4971},
  journal      = {BLOOD},
  keyword      = {THYMUS,NOTCH SIGNALING PATHWAY,MICE,LINEAGE,MARROW,DELTA-1,PRECURSORS,EXPRESSION,IN-VIVO,FATE},
  language     = {eng},
  number       = {7},
  pages        = {2879--2881},
  title        = {Overexpression of HES-1 is not sufficient to impose T-cell differentiation on human hernatopoietic stem cells},
  url          = {http://dx.doi.org/10.1182/blood-2005-05-1815},
  volume       = {107},
  year         = {2006},
}

Chicago
Hoebeke, Inge, Magda De Smedt, Inge Van de Walle, Katia Reynvoet, Greet De Smet, Jean Plum, and Georges Leclercq. 2006. “Overexpression of HES-1 Is Not Sufficient to Impose T-cell Differentiation on Human Hernatopoietic Stem Cells.” Blood 107 (7): 2879–2881.
APA
Hoebeke, I., De Smedt, M., Van de Walle, I., Reynvoet, K., De Smet, G., Plum, J., & Leclercq, G. (2006). Overexpression of HES-1 is not sufficient to impose T-cell differentiation on human hernatopoietic stem cells. BLOOD, 107(7), 2879–2881.
Vancouver
1.
Hoebeke I, De Smedt M, Van de Walle I, Reynvoet K, De Smet G, Plum J, et al. Overexpression of HES-1 is not sufficient to impose T-cell differentiation on human hernatopoietic stem cells. BLOOD. 2006;107(7):2879–81.
MLA
Hoebeke, Inge, Magda De Smedt, Inge Van de Walle, et al. “Overexpression of HES-1 Is Not Sufficient to Impose T-cell Differentiation on Human Hernatopoietic Stem Cells.” BLOOD 107.7 (2006): 2879–2881. Print.