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Overexpression of HES-1 is not sufficient to impose T-cell differentiation on human hernatopoietic stem cells

Inge Hoebeke (UGent) , Magda De Smedt (UGent) , Inge Van de Walle (UGent) , Katia Reynvoet (UGent) , Greet De Smet (UGent) , Jean Plum (UGent) and Georges Leclercq (UGent)
(2006) BLOOD. 107(7). p.2879-2881
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Organization
Abstract
By retroviral overexpression of the Notch-1 intracellular domain (ICN) in human CD34(+) hematopoietic stem cells (HSCs), we have shown previously that Notch-1 signaling promotes the T-cell fate and inhibits the monocyte and B-cell fate in several in vitro and in vivo differentiation assays. Here, we Investigated whether the effects of constitutively active Notch-1 can be mimicked by overexpression of its downstream target gene HES1. Upon HES-1 retroviral transduction, human CD34+ stem cells had a different outcome in the differentiation assays as compared to ICN-transduced cells. Although HES-1 induced a partial block in B-cell development, it did not inhibit monocyte development and did not promote T/NK-cell-lineage differentiation. On the contrary, a higher percentage of HES-1-transduced stem cells remained CD34+. These experiments indicate that HES-1 alone is not able to substitute for Notch-1 signaling to induce T-cell differentiation of human CD34(+) hematopoietic stem cells.
Keywords
THYMUS, NOTCH SIGNALING PATHWAY, MICE, LINEAGE, MARROW, DELTA-1, PRECURSORS, EXPRESSION, IN-VIVO, FATE

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Chicago
Hoebeke, Inge, Magda De Smedt, Inge Van de Walle, Katia Reynvoet, Greet De Smet, Jean Plum, and Georges Leclercq. 2006. “Overexpression of HES-1 Is Not Sufficient to Impose T-cell Differentiation on Human Hernatopoietic Stem Cells.” Blood 107 (7): 2879–2881.
APA
Hoebeke, I., De Smedt, M., Van de Walle, I., Reynvoet, K., De Smet, G., Plum, J., & Leclercq, G. (2006). Overexpression of HES-1 is not sufficient to impose T-cell differentiation on human hernatopoietic stem cells. BLOOD, 107(7), 2879–2881.
Vancouver
1.
Hoebeke I, De Smedt M, Van de Walle I, Reynvoet K, De Smet G, Plum J, et al. Overexpression of HES-1 is not sufficient to impose T-cell differentiation on human hernatopoietic stem cells. BLOOD. 2006;107(7):2879–81.
MLA
Hoebeke, Inge, Magda De Smedt, Inge Van de Walle, et al. “Overexpression of HES-1 Is Not Sufficient to Impose T-cell Differentiation on Human Hernatopoietic Stem Cells.” BLOOD 107.7 (2006): 2879–2881. Print.
@article{338358,
  abstract     = {By retroviral overexpression of the Notch-1 intracellular domain (ICN) in human CD34(+) hematopoietic stem cells (HSCs), we have shown previously that Notch-1 signaling promotes the T-cell fate and inhibits the monocyte and B-cell fate in several in vitro and in vivo differentiation assays. Here, we Investigated whether the effects of constitutively active Notch-1 can be mimicked by overexpression of its downstream target gene HES1. Upon HES-1 retroviral transduction, human CD34+ stem cells had a different outcome in the differentiation assays as compared to ICN-transduced cells. Although HES-1 induced a partial block in B-cell development, it did not inhibit monocyte development and did not promote T/NK-cell-lineage differentiation. On the contrary, a higher percentage of HES-1-transduced stem cells remained CD34+. These experiments indicate that HES-1 alone is not able to substitute for Notch-1 signaling to induce T-cell differentiation of human CD34(+) hematopoietic stem cells.},
  author       = {Hoebeke, Inge and De Smedt, Magda and Van de Walle, Inge and Reynvoet, Katia and De Smet, Greet and Plum, Jean and Leclercq, Georges},
  issn         = {0006-4971},
  journal      = {BLOOD},
  language     = {eng},
  number       = {7},
  pages        = {2879--2881},
  title        = {Overexpression of HES-1 is not sufficient to impose T-cell differentiation on human hernatopoietic stem cells},
  url          = {http://dx.doi.org/10.1182/blood-2005-05-1815},
  volume       = {107},
  year         = {2006},
}

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