Advanced search
1 file | 199.78 KB

Detection of putative secreted proteins in the plant-parasitic nematode Heterodera schachtii

(2006) PARASITOLOGY RESEARCH. 98(5). p.414-424
Author
Organization
Abstract
The beet cyst nematode Heterodera schachtii is an important pathogen worldwide, but its molecular characterization has been limited to studying individual genes of interest. We undertook a high-throughput genomic approach and drastically increased the number of available sequences for this parasite. A total of 2,662 expressed sequence tags were grouped into 1,212 clusters representing a nonredundant catalog of H. schachtii genes. Implementing a bioinformatic workflow, we identified 50 sequences coding for candidate secreted proteins. All of these contain a putative signal peptide required for entry into the secretory pathway and lack any transmembrane domain. Included are previously postulated cell-wall-degrading enzymes and other parasitism-related genes. Moreover, we provide the first report of an arabinogalactan endo-1,4-beta-galactosidase enzyme (EC 3.2.1.89) in animals. As sequence data increase at a rapid rate, developing high-throughput genomic screening is a necessity. The in silico approach described here is an effective way to identify putative secreted proteins and prioritize candidates for further studies.
Keywords
ROOT-KNOT NEMATODE, FREE-LIVING NEMATODES, MELOIDOGYNE-INCOGNITA, DEVELOPMENTAL EXPRESSION, GENE-EXPRESSION, CYST-NEMATODES, MOLECULAR CHARACTERIZATION, GLOBODERA-ROSTOCHIENSIS, VENOM ALLERGEN, GLYCINES

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 199.78 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Vanholme, Bartel, M Mitreva, Wim Van Criekinge, Marc Logghe, D Bird, JP McCarter, and Godelieve Gheysen. 2006. “Detection of Putative Secreted Proteins in the Plant-parasitic Nematode Heterodera Schachtii.” Parasitology Research 98 (5): 414–424.
APA
Vanholme, B., Mitreva, M., Van Criekinge, W., Logghe, M., Bird, D., McCarter, J., & Gheysen, G. (2006). Detection of putative secreted proteins in the plant-parasitic nematode Heterodera schachtii. PARASITOLOGY RESEARCH, 98(5), 414–424.
Vancouver
1.
Vanholme B, Mitreva M, Van Criekinge W, Logghe M, Bird D, McCarter J, et al. Detection of putative secreted proteins in the plant-parasitic nematode Heterodera schachtii. PARASITOLOGY RESEARCH. 2006;98(5):414–24.
MLA
Vanholme, Bartel, M Mitreva, Wim Van Criekinge, et al. “Detection of Putative Secreted Proteins in the Plant-parasitic Nematode Heterodera Schachtii.” PARASITOLOGY RESEARCH 98.5 (2006): 414–424. Print.
@article{334373,
  abstract     = {The beet cyst nematode Heterodera schachtii is an important pathogen worldwide, but its molecular characterization has been limited to studying individual genes of interest. We undertook a high-throughput genomic approach and drastically increased the number of available sequences for this parasite. A total of 2,662 expressed sequence tags were grouped into 1,212 clusters representing a nonredundant catalog of H. schachtii genes. Implementing a bioinformatic workflow, we identified 50 sequences coding for candidate secreted proteins. All of these contain a putative signal peptide required for entry into the secretory pathway and lack any transmembrane domain. Included are previously postulated cell-wall-degrading enzymes and other parasitism-related genes. Moreover, we provide the first report of an arabinogalactan endo-1,4-beta-galactosidase enzyme (EC 3.2.1.89) in animals. As sequence data increase at a rapid rate, developing high-throughput genomic screening is a necessity. The in silico approach described here is an effective way to identify putative secreted proteins and prioritize candidates for further studies.},
  author       = {Vanholme, Bartel and Mitreva, M and Van Criekinge, Wim and Logghe, Marc and Bird, D and McCarter, JP and Gheysen, Godelieve},
  issn         = {0932-0113},
  journal      = {PARASITOLOGY RESEARCH},
  keyword      = {ROOT-KNOT NEMATODE,FREE-LIVING NEMATODES,MELOIDOGYNE-INCOGNITA,DEVELOPMENTAL EXPRESSION,GENE-EXPRESSION,CYST-NEMATODES,MOLECULAR CHARACTERIZATION,GLOBODERA-ROSTOCHIENSIS,VENOM ALLERGEN,GLYCINES},
  language     = {eng},
  number       = {5},
  pages        = {414--424},
  title        = {Detection of putative secreted proteins in the plant-parasitic nematode Heterodera schachtii},
  volume       = {98},
  year         = {2006},
}

Web of Science
Times cited: