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Capabilities and limitations of gel electrophoresis for elemental speciation : a laboratory's experience

Cyrille C Chéry, Luc Moens (UGent) , Rita Cornelis (UGent) and Frank Vanhaecke (UGent)
(2006) PURE AND APPLIED CHEMISTRY. 78(1). p.91-103
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Abstract
Gel electrophoresis is a fractionation/separation technique that yields valuable information in the field of metalloproteomics, often referred to as metallomics. This paper is based on four years of practical experience of the authors' lab in this domain and highlights the capabilities and limitations of gel electrophoresis. Pitfalls of the technique were recognized by identifying the origin of artefacts in the separation, species degradation being the most important. Gel electrophoresis can be accomplished under either native or denaturing conditions. The speciation of vanadium and selenium among serum and yeast proteins, respectively, is sued to illustrate these two major modes. The most powerful approach is two-dimensional denaturing gel electrophoresis. This review of the methods used in our laboratory also descries the application of the two major detection techniques, autoradiography on the one hand and electrothermal vaporization - or laser ablation (LA)-inductively coupled plasma-mass spectrometry (ICP-MS) on the other.
Keywords
electrothermal vaporization, gel electrophoresis, laser ablation, autoradiography, vanadium, selenium, ICP-mass spectrometry, speciation, PROTEINS, SELENIUM, YEAST, SPECTROMETRY, MS

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MLA
Chéry, Cyrille C, Luc Moens, Rita Cornelis, et al. “Capabilities and Limitations of Gel Electrophoresis for Elemental Speciation : a Laboratory’s Experience.” PURE AND APPLIED CHEMISTRY 78.1 (2006): 91–103. Print.
APA
Chéry, Cyrille C, Moens, L., Cornelis, R., & Vanhaecke, F. (2006). Capabilities and limitations of gel electrophoresis for elemental speciation : a laboratory’s experience. PURE AND APPLIED CHEMISTRY, 78(1), 91–103. Presented at the 2nd IUPAC symposium on Trace Elements in Food.
Chicago author-date
Chéry, Cyrille C, Luc Moens, Rita Cornelis, and Frank Vanhaecke. 2006. “Capabilities and Limitations of Gel Electrophoresis for Elemental Speciation : a Laboratory’s Experience.” Pure and Applied Chemistry 78 (1): 91–103.
Chicago author-date (all authors)
Chéry, Cyrille C, Luc Moens, Rita Cornelis, and Frank Vanhaecke. 2006. “Capabilities and Limitations of Gel Electrophoresis for Elemental Speciation : a Laboratory’s Experience.” Pure and Applied Chemistry 78 (1): 91–103.
Vancouver
1.
Chéry CC, Moens L, Cornelis R, Vanhaecke F. Capabilities and limitations of gel electrophoresis for elemental speciation : a laboratory’s experience. PURE AND APPLIED CHEMISTRY. 2006;78(1):91–103.
IEEE
[1]
C. C. Chéry, L. Moens, R. Cornelis, and F. Vanhaecke, “Capabilities and limitations of gel electrophoresis for elemental speciation : a laboratory’s experience,” PURE AND APPLIED CHEMISTRY, vol. 78, no. 1, pp. 91–103, 2006.
@article{331980,
  abstract     = {Gel electrophoresis is a fractionation/separation technique that yields valuable information in the field of metalloproteomics, often referred to as metallomics. This paper is based on four years of practical experience of the authors' lab in this domain and highlights the capabilities and limitations of gel electrophoresis. Pitfalls of the technique were recognized by identifying the origin of artefacts in the separation, species degradation being the most important. Gel electrophoresis can be accomplished under either native or denaturing conditions. The speciation of vanadium and selenium among serum and yeast proteins, respectively, is sued to illustrate these two major modes. The most powerful approach is two-dimensional denaturing gel electrophoresis. This review of the methods used in our laboratory also descries the application of the two major detection techniques, autoradiography on the one hand and electrothermal vaporization - or laser ablation (LA)-inductively coupled plasma-mass spectrometry (ICP-MS) on the other.},
  author       = {Chéry, Cyrille C and Moens, Luc and Cornelis, Rita and Vanhaecke, Frank},
  issn         = {0033-4545},
  journal      = {PURE AND APPLIED CHEMISTRY},
  keywords     = {electrothermal vaporization,gel electrophoresis,laser ablation,autoradiography,vanadium,selenium,ICP-mass spectrometry,speciation,PROTEINS,SELENIUM,YEAST,SPECTROMETRY,MS},
  language     = {eng},
  location     = {Brussels, Belgium},
  number       = {1},
  pages        = {91--103},
  title        = {Capabilities and limitations of gel electrophoresis for elemental speciation : a laboratory's experience},
  url          = {http://dx.doi.org/10.1351/pac200678010091},
  volume       = {78},
  year         = {2006},
}

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