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Capabilities and limitations of gel electrophoresis for elemental speciation: a laboratory's experience

Cyrille C Chéry, Luc Moens UGent, Rita Cornelis UGent and Frank Vanhaecke UGent (2006) PURE AND APPLIED CHEMISTRY. 78(1). p.91-103
abstract
Gel electrophoresis is a fractionation/separation technique that yields valuable information in the field of metalloproteomics, often referred to as metallomics. This paper is based on four years of practical experience of the authors' lab in this domain and highlights the capabilities and limitations of gel electrophoresis. Pitfalls of the technique were recognized by identifying the origin of artefacts in the separation, species degradation being the most important. Gel electrophoresis can be accomplished under either native or denaturing conditions. The speciation of vanadium and selenium among serum and yeast proteins, respectively, is sued to illustrate these two major modes. The most powerful approach is two-dimensional denaturing gel electrophoresis. This review of the methods used in our laboratory also descries the application of the two major detection techniques, autoradiography on the one hand and electrothermal vaporization - or laser ablation (LA)-inductively coupled plasma-mass spectrometry (ICP-MS) on the other.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (proceedingsPaper)
publication status
published
subject
keyword
electrothermal vaporization, gel electrophoresis, laser ablation, autoradiography, vanadium, selenium, ICP-mass spectrometry, speciation, PROTEINS, SELENIUM, YEAST, SPECTROMETRY, MS
journal title
PURE AND APPLIED CHEMISTRY
Pure Appl. Chem.
volume
78
issue
1
pages
91 - 103
conference name
2nd IUPAC symposium on Trace Elements in Food
conference location
Brussels, Belgium
conference start
2004-10-07
conference end
2004-10-08
Web of Science type
Article
Web of Science id
000234945800010
JCR category
CHEMISTRY, MULTIDISCIPLINARY
JCR impact factor
1.92 (2006)
JCR rank
35/124 (2006)
JCR quartile
2 (2006)
ISSN
0033-4545
DOI
10.1351/pac200678010091
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
331980
handle
http://hdl.handle.net/1854/LU-331980
date created
2006-04-19 15:11:00
date last changed
2012-11-12 11:34:04
@article{331980,
  abstract     = {Gel electrophoresis is a fractionation/separation technique that yields valuable information in the field of metalloproteomics, often referred to as metallomics. This paper is based on four years of practical experience of the authors' lab in this domain and highlights the capabilities and limitations of gel electrophoresis. Pitfalls of the technique were recognized by identifying the origin of artefacts in the separation, species degradation being the most important. Gel electrophoresis can be accomplished under either native or denaturing conditions. The speciation of vanadium and selenium among serum and yeast proteins, respectively, is sued to illustrate these two major modes. The most powerful approach is two-dimensional denaturing gel electrophoresis. This review of the methods used in our laboratory also descries the application of the two major detection techniques, autoradiography on the one hand and electrothermal vaporization - or laser ablation (LA)-inductively coupled plasma-mass spectrometry (ICP-MS) on the other.},
  author       = {Ch{\'e}ry, Cyrille C and Moens, Luc and Cornelis, Rita and Vanhaecke, Frank},
  issn         = {0033-4545},
  journal      = {PURE AND APPLIED CHEMISTRY},
  keyword      = {electrothermal vaporization,gel electrophoresis,laser ablation,autoradiography,vanadium,selenium,ICP-mass spectrometry,speciation,PROTEINS,SELENIUM,YEAST,SPECTROMETRY,MS},
  language     = {eng},
  location     = {Brussels, Belgium},
  number       = {1},
  pages        = {91--103},
  title        = {Capabilities and limitations of gel electrophoresis for elemental speciation: a laboratory's experience},
  url          = {http://dx.doi.org/10.1351/pac200678010091},
  volume       = {78},
  year         = {2006},
}

Chicago
Chéry, Cyrille C, Luc Moens, Rita Cornelis, and Frank Vanhaecke. 2006. “Capabilities and Limitations of Gel Electrophoresis for Elemental Speciation: a Laboratory’s Experience.” Pure and Applied Chemistry 78 (1): 91–103.
APA
Chéry, C. C., Moens, L., Cornelis, R., & Vanhaecke, F. (2006). Capabilities and limitations of gel electrophoresis for elemental speciation: a laboratory’s experience. PURE AND APPLIED CHEMISTRY, 78(1), 91–103. Presented at the 2nd IUPAC symposium on Trace Elements in Food.
Vancouver
1.
Chéry CC, Moens L, Cornelis R, Vanhaecke F. Capabilities and limitations of gel electrophoresis for elemental speciation: a laboratory’s experience. PURE AND APPLIED CHEMISTRY. 2006;78(1):91–103.
MLA
Chéry, Cyrille C, Luc Moens, Rita Cornelis, et al. “Capabilities and Limitations of Gel Electrophoresis for Elemental Speciation: a Laboratory’s Experience.” PURE AND APPLIED CHEMISTRY 78.1 (2006): 91–103. Print.