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The influence of pre-cultural conditions on the individual cell lag phase and generation time of Listeria monocytogenes

A Valero, R García-Gimeno, G Zurera, Kjell Francois, Frank Devlieghere UGent and Johan Debevere (2005) Acta Horticulturae. 674. p.441-450
abstract
In this study, we assessed the impact of pre-cultural temperature and pH on the lag phase of individual Listeria monocytogenes cells at 7 degrees C. In a first step, the pre-cultural temperature effect (37, 15, 10, 7, 4 and 2 degrees C) was investigated on a subsequent growth at 7 degrees C and pH 7.4. In a second step, low pre-cultural temperatures (10, 7 and 4 degrees C) were combined with a controlled pH at 7.4 and 5.7 with a subsequent growth at 7 degrees C and different pH values (7.4, 6.0 and 5.5). Growth was monitored by OD measurements at 600 nm. For all temperature-pH combinations, the individual cell lag phase and the subsequent growth rate were determined using a three-phase linear growth model. Around 100 replications were made for each set of conditions. The results were plotted as histograms, and distributions were fitted to these data. It was observed that at low pre-cultural temperatures, a high proportion of L. monocytogenes cells was able to grow with almost no lag phase. Regarding the pH effect, the pH transition from the pre-cultural to a growth media was proportional to the mean values of the lag phases. There was no remarkable effect observed on the generation time. Regarding the statistical distributions, the exponential distribution yielded the best fit in most cases, as the highest probability was observed for very small lag phases and decreased with lag phase increase. To apply the results obtained at an industrial level, a simulation in @RISK to predict the distribution of the lag phase of L. monocytogenes at different transitions of temperature was performed. These findings have important consequences to estimate the possible risks concerned L. monocytogenes for refrigerated food products.
Please use this url to cite or link to this publication:
author
organization
year
type
conference (proceedingsPaper)
publication status
published
subject
keyword
physiological state, effect of history of cells, growth parameters, statistical distributions, Microbiological Risk Assessment (MRA), TEMPERATURE HISTORY, BACTERIAL-GROWTH
in
Acta Horticulturae
Acta Hortic.
editor
BM Nicolaï, MLATM Hertog and LMM Tijskens
volume
674
issue title
III International symposium on applications of modelling as an innovative technology in the agri-food chain : MODEL-IT
pages
441 - 450
publisher
International Society for Horticultural Science (ISHS)
place of publication
Leuven, Belgium
conference name
3rd International symposium on Applications of Modelling as an Innovative Technology in the Agri-Food Chain
conference location
Leuven, Belgium
conference start
2005-05-29
conference end
2005-06-02
Web of Science type
Proceedings Paper
Web of Science id
000230885900057
ISSN
0567-7572
ISBN
9789066055186
language
English
UGent publication?
yes
classification
P1
id
331518
handle
http://hdl.handle.net/1854/LU-331518
date created
2006-04-14 15:23:00
date last changed
2017-05-02 12:07:04
@inproceedings{331518,
  abstract     = {In this study, we assessed the impact of pre-cultural temperature and pH on the lag phase of individual Listeria monocytogenes cells at 7 degrees C. In a first step, the pre-cultural temperature effect (37, 15, 10, 7, 4 and 2 degrees C) was investigated on a subsequent growth at 7 degrees C and pH 7.4. In a second step, low pre-cultural temperatures (10, 7 and 4 degrees C) were combined with a controlled pH at 7.4 and 5.7 with a subsequent growth at 7 degrees C and different pH values (7.4, 6.0 and 5.5). Growth was monitored by OD measurements at 600 nm. For all temperature-pH combinations, the individual cell lag phase and the subsequent growth rate were determined using a three-phase linear growth model. Around 100 replications were made for each set of conditions. The results were plotted as histograms, and distributions were fitted to these data. It was observed that at low pre-cultural temperatures, a high proportion of L. monocytogenes cells was able to grow with almost no lag phase. Regarding the pH effect, the pH transition from the pre-cultural to a growth media was proportional to the mean values of the lag phases. There was no remarkable effect observed on the generation time. Regarding the statistical distributions, the exponential distribution yielded the best fit in most cases, as the highest probability was observed for very small lag phases and decreased with lag phase increase. To apply the results obtained at an industrial level, a simulation in @RISK to predict the distribution of the lag phase of L. monocytogenes at different transitions of temperature was performed. These findings have important consequences to estimate the possible risks concerned L. monocytogenes for refrigerated food products.},
  author       = {Valero, A and Garc{\'i}a-Gimeno, R and Zurera, G and Francois, Kjell and Devlieghere, Frank and Debevere, Johan},
  booktitle    = {Acta Horticulturae},
  editor       = {Nicola{\"i}, BM and Hertog, MLATM and Tijskens, LMM},
  isbn         = {9789066055186},
  issn         = {0567-7572},
  keyword      = {physiological state,effect of history of cells,growth parameters,statistical distributions,Microbiological Risk Assessment (MRA),TEMPERATURE HISTORY,BACTERIAL-GROWTH},
  language     = {eng},
  location     = {Leuven, Belgium},
  pages        = {441--450},
  publisher    = {International Society for Horticultural Science (ISHS)},
  title        = {The influence of pre-cultural conditions on the individual cell lag phase and generation time of Listeria monocytogenes},
  volume       = {674},
  year         = {2005},
}

Chicago
Valero, A, R García-Gimeno, G Zurera, Kjell Francois, Frank Devlieghere, and Johan Debevere. 2005. “The Influence of Pre-cultural Conditions on the Individual Cell Lag Phase and Generation Time of Listeria Monocytogenes.” In Acta Horticulturae, ed. BM Nicolaï, MLATM Hertog, and LMM Tijskens, 674:441–450. Leuven, Belgium: International Society for Horticultural Science (ISHS).
APA
Valero, A., García-Gimeno, R., Zurera, G., Francois, K., Devlieghere, F., & Debevere, J. (2005). The influence of pre-cultural conditions on the individual cell lag phase and generation time of Listeria monocytogenes. In B. Nicolaï, M. Hertog, & L. Tijskens (Eds.), Acta Horticulturae (Vol. 674, pp. 441–450). Presented at the 3rd International symposium on Applications of Modelling as an Innovative Technology in the Agri-Food Chain, Leuven, Belgium: International Society for Horticultural Science (ISHS).
Vancouver
1.
Valero A, García-Gimeno R, Zurera G, Francois K, Devlieghere F, Debevere J. The influence of pre-cultural conditions on the individual cell lag phase and generation time of Listeria monocytogenes. In: Nicolaï B, Hertog M, Tijskens L, editors. Acta Horticulturae. Leuven, Belgium: International Society for Horticultural Science (ISHS); 2005. p. 441–50.
MLA
Valero, A, R García-Gimeno, G Zurera, et al. “The Influence of Pre-cultural Conditions on the Individual Cell Lag Phase and Generation Time of Listeria Monocytogenes.” Acta Horticulturae. Ed. BM Nicolaï, MLATM Hertog, & LMM Tijskens. Vol. 674. Leuven, Belgium: International Society for Horticultural Science (ISHS), 2005. 441–450. Print.