Advanced search

Modelling the effect of environmental parameters (temperature, pH, aw) on the individual cell lag phase of Listeria monocytogenes

(2005) Acta Horticulturae. 674. p.39-48
Author
Organization
Abstract
The effect of the environmental factors temperature (2-30 degrees C), pH (4.4-7.4) and a(X) (0.995-0.947) on the individual cell lag phase of Listeria monocytogenes was investigated. Individual cells were isolated using a serial dilution protocol in microtiter plates, and subsequent growth was investigated by optical density (OD) measurements. About 100 replicates were made for each set of environmental conditions. Individual cell lag phases and subsequent growth rates were calculated using a linear growth model. The results were discussed as mean values to determine the general trends, but also histograms were made and statistical distributions were fitted to the different data sets. The best descriptions of the data were obtained with an exponential distribution for low stress levels, a gamma distribution for intermediate stress and a Weibull distribution for severe stress levels. When only low stress levels were applied, a significant percentage of the cells showed no significant lag. In those cases better fits are obtained by separating cells with lag and those without lag using a binomial distribution and in a second step, fitting a gamma or a Weibull distribution to the fraction showing significant lag.
Keywords
Listeria monocytogenes, individual cell lag phase, risk assessment, optical density, predictive modelling, BACTERIAL-GROWTH

Citation

Please use this url to cite or link to this publication:

Chicago
Francois, Kjell, Frank Devlieghere, Koen Smet, A Standaert, AH Geeraerd, Jan Van Impe, and Johan Debevere. 2005. “Modelling the Effect of Environmental Parameters (temperature, pH, Aw) on the Individual Cell Lag Phase of Listeria Monocytogenes.” In Acta Horticulturae, ed. MLA Hertog, BM Nicolaï, and LMM Tijskens, 674:39–48. Leuven, Belgium: International Society for Horticultural Science (ISHS).
APA
Francois, Kjell, Devlieghere, F., Smet, K., Standaert, A., Geeraerd, A., Van Impe, J., & Debevere, J. (2005). Modelling the effect of environmental parameters (temperature, pH, aw) on the individual cell lag phase of Listeria monocytogenes. In M. Hertog, B. Nicolaï, & L. Tijskens (Eds.), Acta Horticulturae (Vol. 674, pp. 39–48). Presented at the 3rd International symposium on Applications of Modelling as an Innovative Technology in the Agri-Food Chain, Leuven, Belgium: International Society for Horticultural Science (ISHS).
Vancouver
1.
Francois K, Devlieghere F, Smet K, Standaert A, Geeraerd A, Van Impe J, et al. Modelling the effect of environmental parameters (temperature, pH, aw) on the individual cell lag phase of Listeria monocytogenes. In: Hertog M, Nicolaï B, Tijskens L, editors. Acta Horticulturae. Leuven, Belgium: International Society for Horticultural Science (ISHS); 2005. p. 39–48.
MLA
Francois, Kjell, Frank Devlieghere, Koen Smet, et al. “Modelling the Effect of Environmental Parameters (temperature, pH, Aw) on the Individual Cell Lag Phase of Listeria Monocytogenes.” Acta Horticulturae. Ed. MLA Hertog, BM Nicolaï, & LMM Tijskens. Vol. 674. Leuven, Belgium: International Society for Horticultural Science (ISHS), 2005. 39–48. Print.
@inproceedings{331486,
  abstract     = {The effect of the environmental factors temperature (2-30 degrees C), pH (4.4-7.4) and a(X) (0.995-0.947) on the individual cell lag phase of Listeria monocytogenes was investigated. Individual cells were isolated using a serial dilution protocol in microtiter plates, and subsequent growth was investigated by optical density (OD) measurements. About 100 replicates were made for each set of environmental conditions. Individual cell lag phases and subsequent growth rates were calculated using a linear growth model. 
The results were discussed as mean values to determine the general trends, but also histograms were made and statistical distributions were fitted to the different data sets. The best descriptions of the data were obtained with an exponential distribution for low stress levels, a gamma distribution for intermediate stress and a Weibull distribution for severe stress levels. When only low stress levels were applied, a significant percentage of the cells showed no significant lag. In those cases better fits are obtained by separating cells with lag and those without lag using a binomial distribution and in a second step, fitting a gamma or a Weibull distribution to the fraction showing significant lag.},
  author       = {Francois, Kjell and Devlieghere, Frank and Smet, Koen and Standaert, A and Geeraerd, AH and Van Impe, Jan and Debevere, Johan},
  booktitle    = {Acta Horticulturae},
  editor       = {Hertog, MLA and Nicola{\"i}, BM and Tijskens, LMM},
  isbn         = {9789066055186},
  issn         = {0567-7572},
  keyword      = {Listeria monocytogenes,individual cell lag phase,risk assessment,optical density,predictive modelling,BACTERIAL-GROWTH},
  language     = {eng},
  location     = {Leuven, Belgium},
  pages        = {39--48},
  publisher    = {International Society for Horticultural Science (ISHS)},
  title        = {Modelling the effect of environmental parameters (temperature, pH, aw) on the individual cell lag phase of Listeria monocytogenes},
  url          = {http://www.actahort.org/books/674/674\_3.htm},
  volume       = {674},
  year         = {2005},
}

Web of Science
Times cited: