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Root gravitropism requires lateral root cap and epidermal cells for transport and response to a mobile auxin signal

(2005) NATURE CELL BIOLOGY. 7(11). p.1057-1065
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Abstract
Re-orientation of Arabidopsis seedlings induces a rapid, asymmetric release of the growth regulator auxin from gravity-sensing columella cells at the root apex. The resulting lateral auxin gradient is hypothesized to drive differential cell expansion in elongation-zone tissues. We mapped those root tissues that function to transport or respond to auxin during a gravitropic response. Targeted expression of the auxin influx facilitator AUX1 demonstrated that root gravitropism requires auxin to be transported via the lateral root cap to all elongating epidermal cells. A three-dimensional model of the root elongation zone predicted that AUX1 causes the majority of auxin to accumulate in the epidermis. Selectively disrupting the auxin responsiveness of expanding epidermal cells by expressing a mutant form of the AUX/IAA17 protein, axr3-1, abolished root gravitropism. We conclude that gravitropic curvature in Arabidopsis roots is primarily driven by the differential expansion of epidermal cells in response to an influx-carrier-dependent auxin gradient.
Keywords
MEMBRANE-PROTEIN, ARABIDOPSIS-THALIANA, ELONGATION ZONE, PERMEASE AUX1, GROWTH, GRAVITY, ENCODES, GENE, REGULATOR, TISSUE

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Citation

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Chicago
Swarup, Ranjan, Eric M Kramer, Paula Perry, Kirsten Knox, HM Ottoline Leyser, Jim Haseloff, Gerrit Beemster, Rishikesh Bhalerao, and Malcolm J Bennett. 2005. “Root Gravitropism Requires Lateral Root Cap and Epidermal Cells for Transport and Response to a Mobile Auxin Signal.” Nature Cell Biology 7 (11): 1057–1065.
APA
Swarup, R., Kramer, E. M., Perry, P., Knox, K., Leyser, H. O., Haseloff, J., Beemster, G., et al. (2005). Root gravitropism requires lateral root cap and epidermal cells for transport and response to a mobile auxin signal. NATURE CELL BIOLOGY, 7(11), 1057–1065.
Vancouver
1.
Swarup R, Kramer EM, Perry P, Knox K, Leyser HO, Haseloff J, et al. Root gravitropism requires lateral root cap and epidermal cells for transport and response to a mobile auxin signal. NATURE CELL BIOLOGY. 2005;7(11):1057–65.
MLA
Swarup, Ranjan, Eric M Kramer, Paula Perry, et al. “Root Gravitropism Requires Lateral Root Cap and Epidermal Cells for Transport and Response to a Mobile Auxin Signal.” NATURE CELL BIOLOGY 7.11 (2005): 1057–1065. Print.
@article{331393,
  abstract     = {Re-orientation of Arabidopsis seedlings induces a rapid, asymmetric release of the growth regulator auxin from gravity-sensing columella cells at the root apex. The resulting lateral auxin gradient is hypothesized to drive differential cell expansion in elongation-zone tissues. We mapped those root tissues that function to transport or respond to auxin during a gravitropic response. Targeted expression of the auxin influx facilitator AUX1 demonstrated that root gravitropism requires auxin to be transported via the lateral root cap to all elongating epidermal cells. A three-dimensional model of the root elongation zone predicted that AUX1 causes the majority of auxin to accumulate in the epidermis. Selectively disrupting the auxin responsiveness of expanding epidermal cells by expressing a mutant form of the AUX/IAA17 protein, axr3-1, abolished root gravitropism. We conclude that gravitropic curvature in Arabidopsis roots is primarily driven by the differential expansion of epidermal cells in response to an influx-carrier-dependent auxin gradient.},
  author       = {Swarup, Ranjan and Kramer, Eric M and Perry, Paula and Knox, Kirsten and Leyser, HM Ottoline and Haseloff, Jim and Beemster, Gerrit and Bhalerao, Rishikesh and Bennett, Malcolm J},
  issn         = {1465-7392},
  journal      = {NATURE CELL BIOLOGY},
  keyword      = {MEMBRANE-PROTEIN,ARABIDOPSIS-THALIANA,ELONGATION ZONE,PERMEASE AUX1,GROWTH,GRAVITY,ENCODES,GENE,REGULATOR,TISSUE},
  language     = {eng},
  number       = {11},
  pages        = {1057--1065},
  title        = {Root gravitropism requires lateral root cap and epidermal cells for transport and response to a mobile auxin signal},
  url          = {http://dx.doi.org/10.1038/ncb1316},
  volume       = {7},
  year         = {2005},
}

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