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Molecular changes associated with the setting up of secondary growth in aspen

Damien van Raemdonck, Edouard Pesquet, Sophie Cloquet, Hans Beeckman, Wout Boerjan UGent, Deborah Goffner, Mondher El Jaziri and Marie Baucher UGent (2005) JOURNAL OF EXPERIMENTAL BOTANY. 56(418). p.2211-2227
abstract
Vascular secondary growth results from the activity of the vascular cambium, which produces secondary phloem and secondary xylem. By means of cDNA-amplified fragment length polymorphism (cDNA-AFLP) analysis along aspen stems, several potential regulatory genes involved in the progressive transition from primary to secondary growth were identified. A total of 83 unique transcript-derived fragments (TDFs) was found to be differentiated between the top and the bottom of the stem. An independent RT-PCR expression analysis validated the cDNA-AFLP profiles for 19 of the TDFs. Among these, seven correspond to new genes encoding putative regulatory proteins. Emphasis was laid upon two genes encoding, respectively, an AP2/ERF-like transcription factor (PtaERF1) and a RING finger protein (PtaRHE1); their differential expression was further confirmed by reverse northern analysis. In situ RT-PCR revealed that PtaERF1 was expressed in phloem tissue and that PtaRHE1 had a pronounced expression in ray initials and their derivatives within the cambial zone. These results suggest that these genes have a potential role in vascular tissue development and/or functioning.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PROMOTER, XYLEM, CELL FATE, WOOD FORMATION, FUNCTIONAL GENOMICS, VASCULAR DEVELOPMENT, ARABIDOPSIS-THALIANA, SITU RT-PCR, GENE-EXPRESSION PROFILES, CINNAMYL ALCOHOL-DEHYDROGENASE, vascular cambium, secondary growth, RING-H2, in situ RT-PCR, cDNA-AFLP, AP2/ERF, aspen
journal title
JOURNAL OF EXPERIMENTAL BOTANY
J. Exp. Bot.
volume
56
issue
418
pages
2211 - 2227
Web of Science type
Article
Web of Science id
000230513500023
JCR category
PLANT SCIENCES
JCR impact factor
3.336 (2005)
JCR rank
13/143 (2005)
JCR quartile
1 (2005)
ISSN
0022-0957
DOI
10.1093/jxb/eri221
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
331253
handle
http://hdl.handle.net/1854/LU-331253
date created
2006-04-14 12:12:00
date last changed
2016-12-19 15:44:10
@article{331253,
  abstract     = {Vascular secondary growth results from the activity of the vascular cambium, which produces secondary phloem and secondary xylem. By means of cDNA-amplified fragment length polymorphism (cDNA-AFLP) analysis along aspen stems, several potential regulatory genes involved in the progressive transition from primary to secondary growth were identified. A total of 83 unique transcript-derived fragments (TDFs) was found to be differentiated between the top and the bottom of the stem. An independent RT-PCR expression analysis validated the cDNA-AFLP profiles for 19 of the TDFs. Among these, seven correspond to new genes encoding putative regulatory proteins. Emphasis was laid upon two genes encoding, respectively, an AP2/ERF-like transcription factor (PtaERF1) and a RING finger protein (PtaRHE1); their differential expression was further confirmed by reverse northern analysis. In situ RT-PCR revealed that PtaERF1 was expressed in phloem tissue and that PtaRHE1 had a pronounced expression in ray initials and their derivatives within the cambial zone. These results suggest that these genes have a potential role in vascular tissue development and/or functioning.},
  author       = {van Raemdonck, Damien and Pesquet, Edouard and Cloquet, Sophie and Beeckman, Hans and Boerjan, Wout and Goffner, Deborah and El Jaziri, Mondher and Baucher, Marie},
  issn         = {0022-0957},
  journal      = {JOURNAL OF EXPERIMENTAL BOTANY},
  keyword      = {PROMOTER,XYLEM,CELL FATE,WOOD FORMATION,FUNCTIONAL GENOMICS,VASCULAR DEVELOPMENT,ARABIDOPSIS-THALIANA,SITU RT-PCR,GENE-EXPRESSION PROFILES,CINNAMYL ALCOHOL-DEHYDROGENASE,vascular cambium,secondary growth,RING-H2,in situ RT-PCR,cDNA-AFLP,AP2/ERF,aspen},
  language     = {eng},
  number       = {418},
  pages        = {2211--2227},
  title        = {Molecular changes associated with the setting up of secondary growth in aspen},
  url          = {http://dx.doi.org/10.1093/jxb/eri221},
  volume       = {56},
  year         = {2005},
}

Chicago
van Raemdonck, Damien, Edouard Pesquet, Sophie Cloquet, Hans Beeckman, Wout Boerjan, Deborah Goffner, Mondher El Jaziri, and Marie Baucher. 2005. “Molecular Changes Associated with the Setting up of Secondary Growth in Aspen.” Journal of Experimental Botany 56 (418): 2211–2227.
APA
van Raemdonck, D., Pesquet, E., Cloquet, S., Beeckman, H., Boerjan, W., Goffner, D., El Jaziri, M., et al. (2005). Molecular changes associated with the setting up of secondary growth in aspen. JOURNAL OF EXPERIMENTAL BOTANY, 56(418), 2211–2227.
Vancouver
1.
van Raemdonck D, Pesquet E, Cloquet S, Beeckman H, Boerjan W, Goffner D, et al. Molecular changes associated with the setting up of secondary growth in aspen. JOURNAL OF EXPERIMENTAL BOTANY. 2005;56(418):2211–27.
MLA
van Raemdonck, Damien, Edouard Pesquet, Sophie Cloquet, et al. “Molecular Changes Associated with the Setting up of Secondary Growth in Aspen.” JOURNAL OF EXPERIMENTAL BOTANY 56.418 (2005): 2211–2227. Print.