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The DP-E2F-like gene DEL1 controls the endocycle in Arabidopsis thaliana

Kobe Vlieghe UGent, Véronique Boudolf UGent, Gerrit Beemster UGent, Sara Maes UGent, Zoltán Magyar UGent, Ana Atanassova, Janice de Almeida Engler UGent, Ruth De Groodt UGent, Dirk Inzé UGent and Lieven De Veylder UGent (2005) CURRENT BIOLOGY. 15(1). p.59-63
abstract
Endoreduplication or DNA replication without mitosis is widespread in nature. Well-known examples are fruit fly polytene chromosomes and cereal endosperm. Although endocycles are thought to be driven by the same regulators as those that control the G1-S transition of the mitotic cell cycle, the molecular mechanisms that differentiate mitotically dividing cells from endoreduplicating ones are largely unknown. A novel class of atypical E2F-like proteins has recently been identified and is designated E2F7 in mammals [1, 2] and DP-E2F-like (DEL) in Arabidopsis thaliana [3-5]. We demonstrate that loss of DEL1 function resulted in increased ploidy levels, whereas ectopic expression of DEL1 reduced endoreduplication. Ploidy changes were correlated with altered expression of a subset of E2F target genes encoding proteins necessary for DNA replication. Because DEL1 proteins were postulated to antagonize the E2F pathway, we generated DEL1-E2Fa-DPa triple transgenics. DEL1 inhibited the endoreduplication phenotype, but not the ectopic cell divisions that resulted from the overexpression of both E2Fa and DPa, illustrating that DEL1 specifically represses the endocycle. Because DEL1 transcripts were detected exclusively in mitotically dividing cells, we conclude that DEL1 is an important novel inhibitor of the endocycle and preserves the mitotic state of proliferating cells by suppressing transcription of genes that are required for cells to enter the DNA endoreduplication cycle.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PLANTS, GROWTH, HYPOCOTYL, PROLIFERATION, DIFFERENTIATION, ENDOREDUPLICATION, E2F FAMILY, TRANSCRIPTION FACTOR
journal title
CURRENT BIOLOGY
Curr. Biol.
volume
15
issue
1
pages
59 - 63
Web of Science type
Article
Web of Science id
000226715000026
JCR category
BIOCHEMISTRY & MOLECULAR BIOLOGY
JCR impact factor
11.732 (2005)
JCR rank
11/256 (2005)
JCR quartile
1 (2005)
ISSN
0960-9822
DOI
10.1016/j.cub.2004.12.038
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
331017
handle
http://hdl.handle.net/1854/LU-331017
date created
2006-04-13 19:11:00
date last changed
2013-10-11 12:00:58
@article{331017,
  abstract     = {Endoreduplication or DNA replication without mitosis is widespread in nature. Well-known examples are fruit fly polytene chromosomes and cereal endosperm. Although endocycles are thought to be driven by the same regulators as those that control the G1-S transition of the mitotic cell cycle, the molecular mechanisms that differentiate mitotically dividing cells from endoreduplicating ones are largely unknown. A novel class of atypical E2F-like proteins has recently been identified and is designated E2F7 in mammals [1, 2] and DP-E2F-like (DEL) in Arabidopsis thaliana [3-5]. We demonstrate that loss of DEL1 function resulted in increased ploidy levels, whereas ectopic expression of DEL1 reduced endoreduplication. Ploidy changes were correlated with altered expression of a subset of E2F target genes encoding proteins necessary for DNA replication. Because DEL1 proteins were postulated to antagonize the E2F pathway, we generated DEL1-E2Fa-DPa triple transgenics. DEL1 inhibited the endoreduplication phenotype, but not the ectopic cell divisions that resulted from the overexpression of both E2Fa and DPa, illustrating that DEL1 specifically represses the endocycle. Because DEL1 transcripts were detected exclusively in mitotically dividing cells, we conclude that DEL1 is an important novel inhibitor of the endocycle and preserves the mitotic state of proliferating cells by suppressing transcription of genes that are required for cells to enter the DNA endoreduplication cycle.},
  author       = {Vlieghe, Kobe and Boudolf, V{\'e}ronique and Beemster, Gerrit and Maes, Sara and Magyar, Zolt{\'a}n and Atanassova, Ana and de Almeida Engler, Janice and De Groodt, Ruth and Inz{\'e}, Dirk and De Veylder, Lieven},
  issn         = {0960-9822},
  journal      = {CURRENT BIOLOGY},
  keyword      = {PLANTS,GROWTH,HYPOCOTYL,PROLIFERATION,DIFFERENTIATION,ENDOREDUPLICATION,E2F FAMILY,TRANSCRIPTION FACTOR},
  language     = {eng},
  number       = {1},
  pages        = {59--63},
  title        = {The DP-E2F-like gene DEL1 controls the endocycle in Arabidopsis thaliana},
  url          = {http://dx.doi.org/10.1016/j.cub.2004.12.038},
  volume       = {15},
  year         = {2005},
}

Chicago
Vlieghe, Kobe, Véronique Boudolf, Gerrit Beemster, Sara Maes, Zoltán Magyar, Ana Atanassova, Janice de Almeida Engler, Ruth De Groodt, Dirk Inzé, and Lieven De Veylder. 2005. “The DP-E2F-like Gene DEL1 Controls the Endocycle in Arabidopsis Thaliana.” Current Biology 15 (1): 59–63.
APA
Vlieghe, K., Boudolf, V., Beemster, G., Maes, S., Magyar, Z., Atanassova, A., de Almeida Engler, J., et al. (2005). The DP-E2F-like gene DEL1 controls the endocycle in Arabidopsis thaliana. CURRENT BIOLOGY, 15(1), 59–63.
Vancouver
1.
Vlieghe K, Boudolf V, Beemster G, Maes S, Magyar Z, Atanassova A, et al. The DP-E2F-like gene DEL1 controls the endocycle in Arabidopsis thaliana. CURRENT BIOLOGY. 2005;15(1):59–63.
MLA
Vlieghe, Kobe, Véronique Boudolf, Gerrit Beemster, et al. “The DP-E2F-like Gene DEL1 Controls the Endocycle in Arabidopsis Thaliana.” CURRENT BIOLOGY 15.1 (2005): 59–63. Print.