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Identification of four adenosine kinase isoforms in tobacco BY-2 cells and their putative role in the cell cycle-regulated cytokinin metabolism

Zuzanna Kwade, Agnieszka Świątek, Abdelkrim Azmi, Alain Goossens UGent, Dirk Inzé UGent, Harry Van Onckelen and Luc Roef (2005) JOURNAL OF BIOLOGICAL CHEMISTRY. 280(17). p.17512-17519
abstract
Adenosine kinase (ADK), a key enzyme in the regulation of the intracellular level of adenosine is also speculated to be responsible for the conversion of cytokinin ribosides to their respective nucleotides. To elucidate the role of ADK in the cytokinin metabolism of tobacco BY-2 cells ( Nicotiana tabacum cv. "Bright Yellow-2"; TBY-2), we have identified and characterized the full-length cDNAs encoding four ADK isoforms of N. tabacum and determined their catalytic properties. The four TBY-2 ADK isoforms ( designated 1S, 2S, 1T, and 2T) display a high affinity for both adenosine ( K-m 1.88 - 7.30 mu M) and three distinct types of cytokinin ribosides: isopentenyladenosine; zeatin riboside; and dihydrozeatin riboside (K-m 0.30 - 8.71 mu M). The V-max/K-m values suggest that ADK2S exhibits in vitro an overall higher efficiency in the metabolism of cytokinin ribosides than the other three isoforms. The expression pattern of NtADK genes is modulated significantly during the cell cycle. We suggest that the increased transcript accumulation of NtADK coupled to an increased ADK activity just prior to mitosis is associated with a very active cytokinin metabolism at that phase of the cell cycle of synchronized TBY-2 cells.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ARABIDOPSIS, PLANTS, PURIFICATION, BIOSYNTHESIS, ABSCISIC-ACID, GENE-EXPRESSION, UNTRANSLATED REGION, QUANTITATIVE-ANALYSIS, MESSENGER-RNA TRANSLATION, ENDOGENOUS CYTOKININS
journal title
JOURNAL OF BIOLOGICAL CHEMISTRY
J. Biol. Chem.
volume
280
issue
17
pages
17512 - 17519
Web of Science type
Article
Web of Science id
000228615500119
JCR category
BIOCHEMISTRY & MOLECULAR BIOLOGY
JCR impact factor
5.854 (2005)
JCR rank
38/256 (2005)
JCR quartile
1 (2005)
ISSN
0021-9258
DOI
10.1074/jbc.M411428200
language
English
UGent publication?
yes
classification
A1
additional info
correction published in J. Biol. Chem. (2005) 280(23), 22555 [http://www.jbc.org/content/280/23/22556.full.pdf+html]
copyright statement
I have transferred the copyright for this publication to the publisher
id
331003
handle
http://hdl.handle.net/1854/LU-331003
date created
2006-04-13 19:03:00
date last changed
2016-12-19 15:41:44
@article{331003,
  abstract     = {Adenosine kinase (ADK), a key enzyme in the regulation of the intracellular level of adenosine is also speculated to be responsible for the conversion of cytokinin ribosides to their respective nucleotides. To elucidate the role of ADK in the cytokinin metabolism of tobacco BY-2 cells ( Nicotiana tabacum cv. {\textacutedbl}Bright Yellow-2{\textacutedbl}; TBY-2), we have identified and characterized the full-length cDNAs encoding four ADK isoforms of N. tabacum and determined their catalytic properties. The four TBY-2 ADK isoforms ( designated 1S, 2S, 1T, and 2T) display a high affinity for both adenosine ( K-m 1.88 - 7.30 mu M) and three distinct types of cytokinin ribosides: isopentenyladenosine; zeatin riboside; and dihydrozeatin riboside (K-m 0.30 - 8.71 mu M). The V-max/K-m values suggest that ADK2S exhibits in vitro an overall higher efficiency in the metabolism of cytokinin ribosides than the other three isoforms. The expression pattern of NtADK genes is modulated significantly during the cell cycle. We suggest that the increased transcript accumulation of NtADK coupled to an increased ADK activity just prior to mitosis is associated with a very active cytokinin metabolism at that phase of the cell cycle of synchronized TBY-2 cells.},
  author       = {Kwade, Zuzanna and \'{S}wi\k{a}tek, Agnieszka and Azmi, Abdelkrim and Goossens, Alain and Inz{\'e}, Dirk and Van Onckelen, Harry and Roef, Luc},
  issn         = {0021-9258},
  journal      = {JOURNAL OF BIOLOGICAL CHEMISTRY},
  keyword      = {ARABIDOPSIS,PLANTS,PURIFICATION,BIOSYNTHESIS,ABSCISIC-ACID,GENE-EXPRESSION,UNTRANSLATED REGION,QUANTITATIVE-ANALYSIS,MESSENGER-RNA TRANSLATION,ENDOGENOUS CYTOKININS},
  language     = {eng},
  number       = {17},
  pages        = {17512--17519},
  title        = {Identification of four adenosine kinase isoforms in tobacco BY-2 cells and their putative role in the cell cycle-regulated cytokinin metabolism},
  url          = {http://dx.doi.org/10.1074/jbc.M411428200},
  volume       = {280},
  year         = {2005},
}

Chicago
Kwade, Zuzanna, Agnieszka Świątek, Abdelkrim Azmi, Alain Goossens, Dirk Inzé, Harry Van Onckelen, and Luc Roef. 2005. “Identification of Four Adenosine Kinase Isoforms in Tobacco BY-2 Cells and Their Putative Role in the Cell Cycle-regulated Cytokinin Metabolism.” Journal of Biological Chemistry 280 (17): 17512–17519.
APA
Kwade, Z., Świątek, A., Azmi, A., Goossens, A., Inzé, D., Van Onckelen, H., & Roef, L. (2005). Identification of four adenosine kinase isoforms in tobacco BY-2 cells and their putative role in the cell cycle-regulated cytokinin metabolism. JOURNAL OF BIOLOGICAL CHEMISTRY, 280(17), 17512–17519.
Vancouver
1.
Kwade Z, Świątek A, Azmi A, Goossens A, Inzé D, Van Onckelen H, et al. Identification of four adenosine kinase isoforms in tobacco BY-2 cells and their putative role in the cell cycle-regulated cytokinin metabolism. JOURNAL OF BIOLOGICAL CHEMISTRY. 2005;280(17):17512–9.
MLA
Kwade, Zuzanna, Agnieszka Świątek, Abdelkrim Azmi, et al. “Identification of Four Adenosine Kinase Isoforms in Tobacco BY-2 Cells and Their Putative Role in the Cell Cycle-regulated Cytokinin Metabolism.” JOURNAL OF BIOLOGICAL CHEMISTRY 280.17 (2005): 17512–17519. Print.