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The elongata mutants identify a functional Elongator complex in plants with a role in cell proliferation during organ growth

Hilde Nelissen UGent, Delphine Fleury, Leonardo Bruno, Pedro Robles, Lieven De Veylder UGent, Jan Traas, José Luis Micol, Marc Van Montagu UGent, Dirk Inzé UGent and Maria Van Lijsebettens UGent (2005) PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 102(21). p.7754-7759
abstract
The key enzyme for transcription of protein-encoding genes in eukaryotes is RNA polymerase II (RNAPII). The recruitment of this enzyme during transcription initiation and its passage along the template during transcription elongation is regulated through the association and dissociation of several complexes. Elongator is a histone acetyl transferase complex, consisting of six subunits (ELP1-ELP6), that copurifies with the elongating RNAPII in yeast and humans. We demonstrate that point mutations in three Arabidopsis thaliana genes, encoding homologs of the yeast Elongator subunits ELP1, ELP3 (histone acetyl transferase), and ELP4 are responsible for the phenotypes of the elongata2 (elo2), elo3, and elo1 mutants, respectively. The elo mutants are characterized by narrow leaves and reduced root growth that results from a decreased cell division rate. Morphological and molecular phenotypes show that the ELONGATA (ELO) genes function in the same biological process and the epistatic interactions between the ELO genes can be explained by the model of complex formation in yeast. Furthermore, the plant Elongator complex is genetically positioned in the process of RNAPII-mediated transcription downstream of Mediator. Our data indicate that the Elongator complex is evolutionarily conserved in structure and function but reveal that the mechanism by which it stimulates cell proliferation is different in yeast and plants.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
histone acetyl transferase complex, Arabidopsis, leaf development, RNA polymerase II, KLUYVEROMYCES-LACTIS ZYMOCIN, SACCHAROMYCES-CEREVISIAE ELONGATOR, POLYMERASE-II HOLOENZYME, ARABIDOPSIS-THALIANA, HISTONE ACETYLTRANSFERASE, FAMILIAL DYSAUTONOMIA, LEAF MORPHOGENESIS, GENE, ACETYLATION, DIVISION
journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Proc. Natl. Acad. Sci. USA
volume
102
issue
21
pages
7754 - 7759
Web of Science type
Article
Web of Science id
000229417500063
JCR category
MULTIDISCIPLINARY SCIENCES
JCR impact factor
10.231 (2005)
JCR rank
3/47 (2005)
JCR quartile
1 (2005)
ISSN
0027-8424
DOI
10.1073/pnas.0502600102
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
330992
handle
http://hdl.handle.net/1854/LU-330992
date created
2006-04-13 18:56:00
date last changed
2012-12-18 15:42:39
@article{330992,
  abstract     = {The key enzyme for transcription of protein-encoding genes in eukaryotes is RNA polymerase II (RNAPII). The recruitment of this enzyme during transcription initiation and its passage along the template during transcription elongation is regulated through the association and dissociation of several complexes. Elongator is a histone acetyl transferase complex, consisting of six subunits (ELP1-ELP6), that copurifies with the elongating RNAPII in yeast and humans. We demonstrate that point mutations in three Arabidopsis thaliana genes, encoding homologs of the yeast Elongator subunits ELP1, ELP3 (histone acetyl transferase), and ELP4 are responsible for the phenotypes of the elongata2 (elo2), elo3, and elo1 mutants, respectively. The elo mutants are characterized by narrow leaves and reduced root growth that results from a decreased cell division rate. Morphological and molecular phenotypes show that the ELONGATA (ELO) genes function in the same biological process and the epistatic interactions between the ELO genes can be explained by the model of complex formation in yeast. Furthermore, the plant Elongator complex is genetically positioned in the process of RNAPII-mediated transcription downstream of Mediator. Our data indicate that the Elongator complex is evolutionarily conserved in structure and function but reveal that the mechanism by which it stimulates cell proliferation is different in yeast and plants.},
  author       = {Nelissen, Hilde and Fleury, Delphine and Bruno, Leonardo and Robles, Pedro and De Veylder, Lieven and Traas, Jan and Micol, Jos{\'e} Luis and Van Montagu, Marc and Inz{\'e}, Dirk and Van Lijsebettens, Maria},
  issn         = {0027-8424},
  journal      = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
  keyword      = {histone acetyl transferase complex,Arabidopsis,leaf development,RNA polymerase II,KLUYVEROMYCES-LACTIS ZYMOCIN,SACCHAROMYCES-CEREVISIAE ELONGATOR,POLYMERASE-II HOLOENZYME,ARABIDOPSIS-THALIANA,HISTONE ACETYLTRANSFERASE,FAMILIAL DYSAUTONOMIA,LEAF MORPHOGENESIS,GENE,ACETYLATION,DIVISION},
  language     = {eng},
  number       = {21},
  pages        = {7754--7759},
  title        = {The elongata mutants identify a functional Elongator complex in plants with a role in cell proliferation during organ growth},
  url          = {http://dx.doi.org/10.1073/pnas.0502600102},
  volume       = {102},
  year         = {2005},
}

Chicago
Nelissen, Hilde, Delphine Fleury, Leonardo Bruno, Pedro Robles, Lieven De Veylder, Jan Traas, José Luis Micol, Marc Van Montagu, Dirk Inzé, and Maria Van Lijsebettens. 2005. “The Elongata Mutants Identify a Functional Elongator Complex in Plants with a Role in Cell Proliferation During Organ Growth.” Proceedings of the National Academy of Sciences of the United States of America 102 (21): 7754–7759.
APA
Nelissen, H., Fleury, D., Bruno, L., Robles, P., De Veylder, L., Traas, J., Micol, J. L., et al. (2005). The elongata mutants identify a functional Elongator complex in plants with a role in cell proliferation during organ growth. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 102(21), 7754–7759.
Vancouver
1.
Nelissen H, Fleury D, Bruno L, Robles P, De Veylder L, Traas J, et al. The elongata mutants identify a functional Elongator complex in plants with a role in cell proliferation during organ growth. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2005;102(21):7754–9.
MLA
Nelissen, Hilde, Delphine Fleury, Leonardo Bruno, et al. “The Elongata Mutants Identify a Functional Elongator Complex in Plants with a Role in Cell Proliferation During Organ Growth.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 102.21 (2005): 7754–7759. Print.