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Methyl jasmonate stimulates the de novo biosynthesis of vitamin C in plant cell suspensions

Beata Wolucka UGent, Alain Goossens UGent and Dirk Inzé UGent (2005) JOURNAL OF EXPERIMENTAL BOTANY. 56(419). p.2527-2538
abstract
Vitamin C (L-ascorbic acid) is an important primary metabolite of plants that functions as an antioxidant, an enzyme cofactor, and a cell-signalling modulator in a wide array of crucial physiological processes, including biosynthesis of the cell wall, secondary metabolites and phytohormones, stress resistance, photoprotection, cell division, and growth. Plants synthesize ascorbic acid via de novo and salvage pathways, but the regulation of its biosynthesis and the mechanisms behind ascorbate homeostasis are largely unknown. Jasmonic acid and its methyl ester (jasmonates) mediate plant responses to many biotic and abiotic stresses by triggering a transcriptional reprogramming that allows cells to cope with pathogens and stress. By using C-14-mannose radiolabelling combined with HPLC and transcript profiling analysis, it is shown that methyl jasmonate treatment increases the de novo synthesis of ascorbic acid in Arabidopsis and tobacco Bright Yellow-2 (BY-2) suspension cells. In BY-2 cells, this stimulation coincides with enhanced transcription of at least two late methyl jasmonate-responsive genes encoding enzymes for vitamin C biosynthesis: the GDP-mannose 3'',5''-epimerase and a putative L-gulono-1,4-lactone dehydrogenase/oxidase. As far as is known, this is the first report of a hormonal regulation of vitamin C biosynthesis in plants. Finally, the role of ascorbic acid in jasmonate-regulated stress responses is reviewed.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
HYDROGEN-PEROXIDE, SEQUENCE-ANALYSIS, TOMATO PLANTS, DEHYDROASCORBATE REDUCTASE, CATHARANTHUS-ROSEUS, GDP-MANNOSE, ARABIDOPSIS-THALIANA, L-ASCORBIC-ACID, GAMMA-LACTONE DEHYDROGENASE, HYDROXYPROLINE-RICH GLYCOPROTEIN, jasmonic acid, hormone signalling, BY-2 cells, Arabidopsis, ascorbic acid
journal title
JOURNAL OF EXPERIMENTAL BOTANY
J. Exp. Bot.
volume
56
issue
419
pages
2527 - 2538
Web of Science type
Article
Web of Science id
000231363000022
JCR category
PLANT SCIENCES
JCR impact factor
3.336 (2005)
JCR rank
13/143 (2005)
JCR quartile
1 (2005)
ISSN
0022-0957
DOI
10.1093/jxb/eri246
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
330976
handle
http://hdl.handle.net/1854/LU-330976
date created
2006-04-13 18:47:00
date last changed
2016-12-19 15:44:10
@article{330976,
  abstract     = {Vitamin C (L-ascorbic acid) is an important primary metabolite of plants that functions as an antioxidant, an enzyme cofactor, and a cell-signalling modulator in a wide array of crucial physiological processes, including biosynthesis of the cell wall, secondary metabolites and phytohormones, stress resistance, photoprotection, cell division, and growth. Plants synthesize ascorbic acid via de novo and salvage pathways, but the regulation of its biosynthesis and the mechanisms behind ascorbate homeostasis are largely unknown. Jasmonic acid and its methyl ester (jasmonates) mediate plant responses to many biotic and abiotic stresses by triggering a transcriptional reprogramming that allows cells to cope with pathogens and stress. By using C-14-mannose radiolabelling combined with HPLC and transcript profiling analysis, it is shown that methyl jasmonate treatment increases the de novo synthesis of ascorbic acid in Arabidopsis and tobacco Bright Yellow-2 (BY-2) suspension cells. In BY-2 cells, this stimulation coincides with enhanced transcription of at least two late methyl jasmonate-responsive genes encoding enzymes for vitamin C biosynthesis: the GDP-mannose 3'',5''-epimerase and a putative L-gulono-1,4-lactone dehydrogenase/oxidase. As far as is known, this is the first report of a hormonal regulation of vitamin C biosynthesis in plants. Finally, the role of ascorbic acid in jasmonate-regulated stress responses is reviewed.},
  author       = {Wolucka, Beata and Goossens, Alain and Inz{\'e}, Dirk},
  issn         = {0022-0957},
  journal      = {JOURNAL OF EXPERIMENTAL BOTANY},
  keyword      = {HYDROGEN-PEROXIDE,SEQUENCE-ANALYSIS,TOMATO PLANTS,DEHYDROASCORBATE REDUCTASE,CATHARANTHUS-ROSEUS,GDP-MANNOSE,ARABIDOPSIS-THALIANA,L-ASCORBIC-ACID,GAMMA-LACTONE DEHYDROGENASE,HYDROXYPROLINE-RICH GLYCOPROTEIN,jasmonic acid,hormone signalling,BY-2 cells,Arabidopsis,ascorbic acid},
  language     = {eng},
  number       = {419},
  pages        = {2527--2538},
  title        = {Methyl jasmonate stimulates the de novo biosynthesis of vitamin C in plant cell suspensions},
  url          = {http://dx.doi.org/10.1093/jxb/eri246},
  volume       = {56},
  year         = {2005},
}

Chicago
Wolucka, Beata, Alain Goossens, and Dirk Inzé. 2005. “Methyl Jasmonate Stimulates the De Novo Biosynthesis of Vitamin C in Plant Cell Suspensions.” Journal of Experimental Botany 56 (419): 2527–2538.
APA
Wolucka, B., Goossens, A., & Inzé, D. (2005). Methyl jasmonate stimulates the de novo biosynthesis of vitamin C in plant cell suspensions. JOURNAL OF EXPERIMENTAL BOTANY, 56(419), 2527–2538.
Vancouver
1.
Wolucka B, Goossens A, Inzé D. Methyl jasmonate stimulates the de novo biosynthesis of vitamin C in plant cell suspensions. JOURNAL OF EXPERIMENTAL BOTANY. 2005;56(419):2527–38.
MLA
Wolucka, Beata, Alain Goossens, and Dirk Inzé. “Methyl Jasmonate Stimulates the De Novo Biosynthesis of Vitamin C in Plant Cell Suspensions.” JOURNAL OF EXPERIMENTAL BOTANY 56.419 (2005): 2527–2538. Print.