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Production of acylated homoserine lactones by Aeromonas and Pseudomonas strains isolated from municipal activated sludge

(2005) CANADIAN JOURNAL OF MICROBIOLOGY. 51(11). p.924-933
Author
Organization
Abstract
Up to now, the production and role of N-acyl homoserine lactones (AHLs) in activated sludge have been poorly understood. In this study, cross-feeding assays with the reporter strains Agrobacterium tumefaciens NTL4 and Chromobacterium violaceum CV026 were used to investigate AHL signal production by municipal activated sludge samples. AHL signal production was consistently detected from municipal activated sludge when different samples were incubated on nutrient media. From one municipal activated sludge sample, 10 strains producing AHL-like auto-inducers were isolated by an overlay technique. 16S rDNA-based phylogenetic analysis showed that eight of the isolates belonged to Aeromonas spp. and two to Pseudomonas spp. Box-PCR indicated that six of these Aeromonas isolates were different strains and the two Pseudomonas strains were identical. The production of AHL or AHL-like compounds by these strains was confirmed by thin layer chromatography and biosensor overlays. The six different Aeromonas strains were found to produce the same set of AHLs, including N-hexanoyl-L-homoserine lactone. These results may indicate the possible presence of AHLs in municipal activated sludge. The potential roles of AHL in this ecosystem are briefly discussed.
Keywords
MICROBIAL COMMUNITY COMPOSITION, ROTATING BIOLOGICAL CONTACTOR, CONTROLS BIOFILM FORMATION, SERRATIA-LIQUEFACIENS MG1, QUORUM-SENSING SIGNALS, 16S RIBOSOMAL-RNA, AGROBACTERIUM-TUMEFACIENS, BACTERIAL BIOFILMS, BIODEGRADATION, IDENTIFICATION, municipal activated sludge, acylated homoserine lactones, Aeromonas spp., bioaggregates, Pseudomonas spp., AHL biosensors

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Chicago
Morgan-Sagastume, F, Nico Boon, Sofie Dobbelaere, Tom Defoirdt, and Willy Verstraete. 2005. “Production of Acylated Homoserine Lactones by Aeromonas and Pseudomonas Strains Isolated from Municipal Activated Sludge.” Canadian Journal of Microbiology 51 (11): 924–933.
APA
Morgan-Sagastume, F, Boon, N., Dobbelaere, S., Defoirdt, T., & Verstraete, W. (2005). Production of acylated homoserine lactones by Aeromonas and Pseudomonas strains isolated from municipal activated sludge. CANADIAN JOURNAL OF MICROBIOLOGY, 51(11), 924–933.
Vancouver
1.
Morgan-Sagastume F, Boon N, Dobbelaere S, Defoirdt T, Verstraete W. Production of acylated homoserine lactones by Aeromonas and Pseudomonas strains isolated from municipal activated sludge. CANADIAN JOURNAL OF MICROBIOLOGY. 2005;51(11):924–33.
MLA
Morgan-Sagastume, F, Nico Boon, Sofie Dobbelaere, et al. “Production of Acylated Homoserine Lactones by Aeromonas and Pseudomonas Strains Isolated from Municipal Activated Sludge.” CANADIAN JOURNAL OF MICROBIOLOGY 51.11 (2005): 924–933. Print.
@article{328775,
  abstract     = {Up to now, the production and role of N-acyl homoserine lactones (AHLs) in activated sludge have been poorly understood. In this study, cross-feeding assays with the reporter strains Agrobacterium tumefaciens NTL4 and Chromobacterium violaceum CV026 were used to investigate AHL signal production by municipal activated sludge samples. AHL signal production was consistently detected from municipal activated sludge when different samples were incubated on nutrient media. From one municipal activated sludge sample, 10 strains producing AHL-like auto-inducers were isolated by an overlay technique. 16S rDNA-based phylogenetic analysis showed that eight of the isolates belonged to Aeromonas spp. and two to Pseudomonas spp. Box-PCR indicated that six of these Aeromonas isolates were different strains and the two Pseudomonas strains were identical. The production of AHL or AHL-like compounds by these strains was confirmed by thin layer chromatography and biosensor overlays. The six different Aeromonas strains were found to produce the same set of AHLs, including N-hexanoyl-L-homoserine lactone. These results may indicate the possible presence of AHLs in municipal activated sludge. The potential roles of AHL in this ecosystem are briefly discussed.},
  author       = {Morgan-Sagastume, F and Boon, Nico and Dobbelaere, Sofie and Defoirdt, Tom and Verstraete, Willy},
  issn         = {0008-4166},
  journal      = {CANADIAN JOURNAL OF MICROBIOLOGY},
  language     = {eng},
  number       = {11},
  pages        = {924--933},
  title        = {Production of acylated homoserine lactones by Aeromonas and Pseudomonas strains isolated from municipal activated sludge},
  url          = {http://dx.doi.org/10.1139/W05-077},
  volume       = {51},
  year         = {2005},
}

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