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Exposure to the proton scavenger glycine under alkaline conditions induces Escherichia coli viability loss

Donna Vanhauteghem UGent, Geert Janssens UGent, Angelo Lauwaerts, Stanislas Sys UGent, Filip Boyen UGent, Eric Cox UGent and Evelyne Meyer UGent (2013) PLOS ONE. 8(3).
abstract
Our previous work described a clear loss of Escherichia coli (E. coli) membrane integrity after incubation with glycine or its N-methylated derivatives N-methylglycine (sarcosine) and N,N-dimethylglycine (DMG), but not N,N,N-trimethylglycine (betaine), under alkaline stress conditions. The current study offers a thorough viability analysis, based on a combination of real-time physiological techniques, of E. coli exposed to glycine and its N-methylated derivatives at alkaline pH. Flow cytometry was applied to assess various physiological parameters such as membrane permeability, esterase activity, respiratory activity and membrane potential. ATP and inorganic phosphate concentrations were also determined. Membrane damage was confirmed through the measurement of nucleic acid leakage. Results further showed no loss of esterase or respiratory activity, while an instant and significant decrease in the ATP concentration occurred upon exposure to either glycine, sarcosine or DMG, but not betaine. There was a clear membrane hyperpolarization as well as a significant increase in cellular inorganic phosphate concentration. Based on these results, we suggest that the inability to sustain an adequate level of ATP combined with a decrease in membrane functionality leads to the loss of bacterial viability when exposed to the proton scavengers glycine, sarcosine and DMG at alkaline pH.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
bacterial viability, flow cytometry, alkaline stress, proton scavenging, betaine, dimethylglycine, sarcosine, glycine
journal title
PLOS ONE
PLoS ONE
volume
8
issue
3
article number
e60328
pages
11 pages
Web of Science type
Article
Web of Science id
000317480700094
JCR category
MULTIDISCIPLINARY SCIENCES
JCR impact factor
3.534 (2013)
JCR rank
8/55 (2013)
JCR quartile
1 (2013)
ISSN
1932-6203
DOI
10.1371/journal.pone.0060328
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
3263311
handle
http://hdl.handle.net/1854/LU-3263311
date created
2013-06-19 13:50:50
date last changed
2016-12-21 15:42:31
@article{3263311,
  abstract     = {Our previous work described a clear loss of Escherichia coli (E. coli) membrane integrity after incubation with glycine or its N-methylated derivatives N-methylglycine (sarcosine) and N,N-dimethylglycine (DMG), but not N,N,N-trimethylglycine (betaine), under alkaline stress conditions. The current study offers a thorough viability analysis, based on a combination of real-time physiological techniques, of E. coli exposed to glycine and its N-methylated derivatives at alkaline pH. Flow cytometry was applied to assess various physiological parameters such as membrane permeability, esterase activity, respiratory activity and membrane potential. ATP and inorganic phosphate concentrations were also determined. Membrane damage was confirmed through the measurement of nucleic acid leakage. Results further showed no loss of esterase or respiratory activity, while an instant and significant decrease in the ATP concentration occurred upon exposure to either glycine, sarcosine or DMG, but not betaine. There was a clear membrane hyperpolarization as well as a significant increase in cellular inorganic phosphate concentration. Based on these results, we suggest that the inability to sustain an adequate level of ATP combined with a decrease in membrane functionality leads to the loss of bacterial viability when exposed to the proton scavengers glycine, sarcosine and DMG at alkaline pH.},
  articleno    = {e60328},
  author       = {Vanhauteghem, Donna and Janssens, Geert and Lauwaerts, Angelo and Sys, Stanislas and Boyen, Filip and Cox, Eric and Meyer, Evelyne},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {bacterial viability,flow cytometry,alkaline stress,proton scavenging,betaine,dimethylglycine,sarcosine,glycine},
  language     = {eng},
  number       = {3},
  pages        = {11},
  title        = {Exposure to the proton scavenger glycine under alkaline conditions induces Escherichia coli viability loss},
  url          = {http://dx.doi.org/10.1371/journal.pone.0060328},
  volume       = {8},
  year         = {2013},
}

Chicago
Vanhauteghem, Donna, Geert Janssens, Angelo Lauwaerts, Stanislas Sys, Filip Boyen, Eric Cox, and Evelyne Meyer. 2013. “Exposure to the Proton Scavenger Glycine Under Alkaline Conditions Induces Escherichia Coli Viability Loss.” Plos One 8 (3).
APA
Vanhauteghem, D., Janssens, G., Lauwaerts, A., Sys, S., Boyen, F., Cox, E., & Meyer, E. (2013). Exposure to the proton scavenger glycine under alkaline conditions induces Escherichia coli viability loss. PLOS ONE, 8(3).
Vancouver
1.
Vanhauteghem D, Janssens G, Lauwaerts A, Sys S, Boyen F, Cox E, et al. Exposure to the proton scavenger glycine under alkaline conditions induces Escherichia coli viability loss. PLOS ONE. 2013;8(3).
MLA
Vanhauteghem, Donna, Geert Janssens, Angelo Lauwaerts, et al. “Exposure to the Proton Scavenger Glycine Under Alkaline Conditions Induces Escherichia Coli Viability Loss.” PLOS ONE 8.3 (2013): n. pag. Print.