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SIP1/ZEB2 induces EMT by repressing genes of different epithelial cell-cell junctions

(2005) NUCLEIC ACIDS RESEARCH. 33(20). p.6566-6578
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Abstract
SIP1/ZEB2 is a member of the delta EF-1 family of two-handed zinc finger nuclear factors. The expression of these transcription factors is associated with epithelial mesenchymal transitions (EMT) during development. SIP1 is also expressed in some breast cancer cell lines and was detected in intestinal gastric carcinomas, where its expression is inversely correlated with that of E-cadherin. Here, we show that expression of SIP1 in human epithelial cells results in a clear morphological change from an epithelial to a mesenchymal phenotype. Induction of this epithelial dedifferentiation was accompanied by repression of several cell junctional proteins, with concomitant repression of their mRNA levels. Besides E-cadherin, other genes coding for crucial proteins of tight junctions, desmosomes and gap junctions were found to be transcriptionally regulated by the transcriptional repressor SIP1. Moreover, study of the promoter regions of selected genes by luciferase reporter assays and chromatin immunoprecipitation shows that repression is directly mediated by SIP1. These data indicate that, during epithelial dedifferentiation, SIP1 represses in a coordinated manner the transcription of genes coding for junctional proteins contributing to the dedifferentiated state; this repression occurs by a general mechanism mediated by Smad Interacting Protein 1 (SIP1)-binding sites.

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MLA
Vandewalle, Cindy, Joke Comijn, Bram De Craene, et al. “SIP1/ZEB2 Induces EMT by Repressing Genes of Different Epithelial Cell-cell Junctions.” NUCLEIC ACIDS RESEARCH 33.20 (2005): 6566–6578. Print.
APA
Vandewalle, Cindy, Comijn, J., De Craene, B., Vermassen, P., Bruyneel, E., Andersen, H., Tulchinsky, E., et al. (2005). SIP1/ZEB2 induces EMT by repressing genes of different epithelial cell-cell junctions. NUCLEIC ACIDS RESEARCH, 33(20), 6566–6578.
Chicago author-date
Vandewalle, Cindy, Joke Comijn, Bram De Craene, Petra Vermassen, Erik Bruyneel, H Andersen, E Tulchinsky, Frans Van Roy, and Geert Berx. 2005. “SIP1/ZEB2 Induces EMT by Repressing Genes of Different Epithelial Cell-cell Junctions.” Nucleic Acids Research 33 (20): 6566–6578.
Chicago author-date (all authors)
Vandewalle, Cindy, Joke Comijn, Bram De Craene, Petra Vermassen, Erik Bruyneel, H Andersen, E Tulchinsky, Frans Van Roy, and Geert Berx. 2005. “SIP1/ZEB2 Induces EMT by Repressing Genes of Different Epithelial Cell-cell Junctions.” Nucleic Acids Research 33 (20): 6566–6578.
Vancouver
1.
Vandewalle C, Comijn J, De Craene B, Vermassen P, Bruyneel E, Andersen H, et al. SIP1/ZEB2 induces EMT by repressing genes of different epithelial cell-cell junctions. NUCLEIC ACIDS RESEARCH. 2005;33(20):6566–78.
IEEE
[1]
C. Vandewalle et al., “SIP1/ZEB2 induces EMT by repressing genes of different epithelial cell-cell junctions,” NUCLEIC ACIDS RESEARCH, vol. 33, no. 20, pp. 6566–6578, 2005.
@article{325324,
  abstract     = {{SIP1/ZEB2 is a member of the delta EF-1 family of two-handed zinc finger nuclear factors. The expression of these transcription factors is associated with epithelial mesenchymal transitions (EMT) during development. SIP1 is also expressed in some breast cancer cell lines and was detected in intestinal gastric carcinomas, where its expression is inversely correlated with that of E-cadherin. Here, we show that expression of SIP1 in human epithelial cells results in a clear morphological change from an epithelial to a mesenchymal phenotype. Induction of this epithelial dedifferentiation was accompanied by repression of several cell junctional proteins, with concomitant repression of their mRNA levels. Besides E-cadherin, other genes coding for crucial proteins of tight junctions, desmosomes and gap junctions were found to be transcriptionally regulated by the transcriptional repressor SIP1. Moreover, study of the promoter regions of selected genes by luciferase reporter assays and chromatin immunoprecipitation shows that repression is directly mediated by SIP1. These data indicate that, during epithelial dedifferentiation, SIP1 represses in a coordinated manner the transcription of genes coding for junctional proteins contributing to the dedifferentiated state; this repression occurs by a general mechanism mediated by Smad Interacting Protein 1 (SIP1)-binding sites.}},
  author       = {{Vandewalle, Cindy and Comijn, Joke and De Craene, Bram and Vermassen, Petra and Bruyneel, Erik and Andersen, H and Tulchinsky, E and Van Roy, Frans and Berx, Geert}},
  issn         = {{0305-1048}},
  journal      = {{NUCLEIC ACIDS RESEARCH}},
  language     = {{eng}},
  number       = {{20}},
  pages        = {{6566--6578}},
  title        = {{SIP1/ZEB2 induces EMT by repressing genes of different epithelial cell-cell junctions}},
  url          = {{http://dx.doi.org/10.1093/nar/gki965}},
  volume       = {{33}},
  year         = {{2005}},
}

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