RNA interference links oxidative stress to the inhibition of heat stress adaptation
- Author
- Zoltán Spiró, Mehmet Alper Arslan, Milán Somogyvári, Minh Tu Nguyen, Arne Smolders (UGent) , Balázs Dancsó, Nóra Németh, Zsuzsanna Elek, Bart Braeckman (UGent) , Péter Csermely and Csaba Sőti
- Organization
- Abstract
- Increased oxidative stress is associated with various diseases and aging, while adaptation to heat stress is an important determinant of survival and contributes to longevity. However, the impact of oxidative stress on heat resistance remains largely unclear. Aim: In this study we investigated how oxidative stress impinges on heat stress responses. Results: We report that hydrogen-peroxide (H2O2) pretreatment inhibits both acquired thermotolerance and heat-induced Hsp70 expression in mammalian cells, as well as acquired thermotolerance in the nematode Caenorhabditis elegans, via RNA interference. Moreover, we demonstrate that elimination of RNA interference by silencing key enzymes in microRNA biogenesis, dcr-1 or pash-1, restores the diminished intrinsic thermotolerance of aged and H2O2-elimination compromised (catalase-2 and peroxiredoxin-2 deficient) worms. Innovation and Conclusion: These results uncover a novel post-transcriptional element in the regulation of heat stress adaptation under oxidative conditions that may have implications in disease susceptibility and aging.
- Keywords
- LIFE-SPAN, CAENORHABDITIS-ELEGANS, DIFFERENTIAL EXPRESSION, SHOCK FACTOR-1, C-ELEGANS, MICRORNA, PROTEIN, GENE, THERMOTOLERANCE, HEAT-SHOCK-PROTEIN-70
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-3239063
- MLA
- Spiró, Zoltán, et al. “RNA Interference Links Oxidative Stress to the Inhibition of Heat Stress Adaptation.” ANTIOXIDANTS & REDOX SIGNALING, vol. 17, no. 6, 2012, pp. 890–901, doi:10.1089/ars.2011.4161.
- APA
- Spiró, Z., Arslan, M. A., Somogyvári, M., Nguyen, M. T., Smolders, A., Dancsó, B., … Sőti, C. (2012). RNA interference links oxidative stress to the inhibition of heat stress adaptation. ANTIOXIDANTS & REDOX SIGNALING, 17(6), 890–901. https://doi.org/10.1089/ars.2011.4161
- Chicago author-date
- Spiró, Zoltán, Mehmet Alper Arslan, Milán Somogyvári, Minh Tu Nguyen, Arne Smolders, Balázs Dancsó, Nóra Németh, et al. 2012. “RNA Interference Links Oxidative Stress to the Inhibition of Heat Stress Adaptation.” ANTIOXIDANTS & REDOX SIGNALING 17 (6): 890–901. https://doi.org/10.1089/ars.2011.4161.
- Chicago author-date (all authors)
- Spiró, Zoltán, Mehmet Alper Arslan, Milán Somogyvári, Minh Tu Nguyen, Arne Smolders, Balázs Dancsó, Nóra Németh, Zsuzsanna Elek, Bart Braeckman, Péter Csermely, and Csaba Sőti. 2012. “RNA Interference Links Oxidative Stress to the Inhibition of Heat Stress Adaptation.” ANTIOXIDANTS & REDOX SIGNALING 17 (6): 890–901. doi:10.1089/ars.2011.4161.
- Vancouver
- 1.Spiró Z, Arslan MA, Somogyvári M, Nguyen MT, Smolders A, Dancsó B, et al. RNA interference links oxidative stress to the inhibition of heat stress adaptation. ANTIOXIDANTS & REDOX SIGNALING. 2012;17(6):890–901.
- IEEE
- [1]Z. Spiró et al., “RNA interference links oxidative stress to the inhibition of heat stress adaptation,” ANTIOXIDANTS & REDOX SIGNALING, vol. 17, no. 6, pp. 890–901, 2012.
@article{3239063,
abstract = {{Increased oxidative stress is associated with various diseases and aging, while adaptation to heat stress is an important determinant of survival and contributes to longevity. However, the impact of oxidative stress on heat resistance remains largely unclear. Aim: In this study we investigated how oxidative stress impinges on heat stress responses. Results: We report that hydrogen-peroxide (H2O2) pretreatment inhibits both acquired thermotolerance and heat-induced Hsp70 expression in mammalian cells, as well as acquired thermotolerance in the nematode Caenorhabditis elegans, via RNA interference. Moreover, we demonstrate that elimination of RNA interference by silencing key enzymes in microRNA biogenesis, dcr-1 or pash-1, restores the diminished intrinsic thermotolerance of aged and H2O2-elimination compromised (catalase-2 and peroxiredoxin-2 deficient) worms. Innovation and Conclusion: These results uncover a novel post-transcriptional element in the regulation of heat stress adaptation under oxidative conditions that may have implications in disease susceptibility and aging.}},
author = {{Spiró, Zoltán and Arslan, Mehmet Alper and Somogyvári, Milán and Nguyen, Minh Tu and Smolders, Arne and Dancsó, Balázs and Németh, Nóra and Elek, Zsuzsanna and Braeckman, Bart and Csermely, Péter and Sőti, Csaba}},
issn = {{1523-0864}},
journal = {{ANTIOXIDANTS & REDOX SIGNALING}},
keywords = {{LIFE-SPAN,CAENORHABDITIS-ELEGANS,DIFFERENTIAL EXPRESSION,SHOCK FACTOR-1,C-ELEGANS,MICRORNA,PROTEIN,GENE,THERMOTOLERANCE,HEAT-SHOCK-PROTEIN-70}},
language = {{eng}},
number = {{6}},
pages = {{890--901}},
title = {{RNA interference links oxidative stress to the inhibition of heat stress adaptation}},
url = {{http://dx.doi.org/10.1089/ars.2011.4161}},
volume = {{17}},
year = {{2012}},
}
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