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A simple instrumental approach for 'supercritical' fluid chromatography in drug discovery and its consequences for coupling with mass spectrometric and light scattering detection

(2011) LC GC NORTH AMERICA. 29(11). p.1006-1016
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Organization
Abstract
In spite of the name supercritical fluid chromatography (SFC), SFC systems are generally operated in the subcritical state. When both the temperature and pressure are below the critical point of the CO(2)-modifier mixture, phase separation may occur, which is claimed to destroy the separation. We show here, however, that working below the critical temperature and pressure has no practical detrimental effect on the separation; its only detrimental effect is on detection by UV. This contribution describes a simple experimental setup applied in recent years in a drug discovery laboratory and illustrates some anomalies and misconceptions about SFC for drug discovery.
Keywords
fixed restrictor, SFC, back pressure regulator, DESIGNING OPERATIONS, STATIONARY PHASES, ISOPYCNIC PLOTS

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Citation

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MLA
Pereira, Alberto, Frank David, Gerd Vanhoenacker, et al. “A Simple Instrumental Approach for ‘Supercritical’ Fluid Chromatography in Drug Discovery and Its Consequences for Coupling with Mass Spectrometric and Light Scattering Detection.” LC GC NORTH AMERICA 29.11 (2011): 1006–1016. Print.
APA
Pereira, Alberto, David, F., Vanhoenacker, G., Brunelli, C., & Sandra, P. (2011). A simple instrumental approach for “supercritical” fluid chromatography in drug discovery and its consequences for coupling with mass spectrometric and light scattering detection. LC GC NORTH AMERICA, 29(11), 1006–1016.
Chicago author-date
Pereira, Alberto, Frank David, Gerd Vanhoenacker, Claudio Brunelli, and Patrick Sandra. 2011. “A Simple Instrumental Approach for ‘Supercritical’ Fluid Chromatography in Drug Discovery and Its Consequences for Coupling with Mass Spectrometric and Light Scattering Detection.” Lc Gc North America 29 (11): 1006–1016.
Chicago author-date (all authors)
Pereira, Alberto, Frank David, Gerd Vanhoenacker, Claudio Brunelli, and Patrick Sandra. 2011. “A Simple Instrumental Approach for ‘Supercritical’ Fluid Chromatography in Drug Discovery and Its Consequences for Coupling with Mass Spectrometric and Light Scattering Detection.” Lc Gc North America 29 (11): 1006–1016.
Vancouver
1.
Pereira A, David F, Vanhoenacker G, Brunelli C, Sandra P. A simple instrumental approach for “supercritical” fluid chromatography in drug discovery and its consequences for coupling with mass spectrometric and light scattering detection. LC GC NORTH AMERICA. 2011;29(11):1006–16.
IEEE
[1]
A. Pereira, F. David, G. Vanhoenacker, C. Brunelli, and P. Sandra, “A simple instrumental approach for ‘supercritical’ fluid chromatography in drug discovery and its consequences for coupling with mass spectrometric and light scattering detection,” LC GC NORTH AMERICA, vol. 29, no. 11, pp. 1006–1016, 2011.
@article{3233323,
  abstract     = {In spite of the name supercritical fluid chromatography (SFC), SFC systems are generally operated in the subcritical state. When both the temperature and pressure are below the critical point of the CO(2)-modifier mixture, phase separation may occur, which is claimed to destroy the separation. We show here, however, that working below the critical temperature and pressure has no practical detrimental effect on the separation; its only detrimental effect is on detection by UV. This contribution describes a simple experimental setup applied in recent years in a drug discovery laboratory and illustrates some anomalies and misconceptions about SFC for drug discovery.},
  author       = {Pereira, Alberto and David, Frank and Vanhoenacker, Gerd and Brunelli, Claudio and Sandra, Patrick},
  issn         = {1527-5949},
  journal      = {LC GC NORTH AMERICA},
  keywords     = {fixed restrictor,SFC,back pressure regulator,DESIGNING OPERATIONS,STATIONARY PHASES,ISOPYCNIC PLOTS},
  language     = {eng},
  number       = {11},
  pages        = {1006--1016},
  title        = {A simple instrumental approach for 'supercritical' fluid chromatography in drug discovery and its consequences for coupling with mass spectrometric and light scattering detection},
  volume       = {29},
  year         = {2011},
}

Web of Science
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