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An explorative study on the cell wall polysaccharides in the pulp and peel of dragon fruits (Hylocereus spp.)

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Abstract
The pectic and hemicellulosic cell wall polysaccharides from the pulp and the peel of white-flesh and red-flesh dragon fruits (Hylocereus spp.) were isolated and compared in terms of degree of methoxylation (DM), solubility properties (relative content of uronic acids and neutral sugars in different fractions), neutral sugar composition, molar mass distribution, and affinity towards some specific anti-pectin antibodies. Hereto, the alcohol insoluble residues were extracted and sequentially fractionated using hot water, a chelating agent, sodium carbonate, and potassium hydroxide solutions to obtain different pectin fractions and a hemicellulose fraction. Chemical analyses were used to characterize these polysaccharide fractions. The results show that cell wall polysaccharides of the pulp and especially of the peel from white-flesh and red-flesh dragon fruits contain significant amounts of pectic substances that are lowly methyl-esterified. The cell wall polysaccharides of the peel as well as those of the pulp contain high amounts (38-47%) of loosely bound (water-soluble) pectic substances. In the water-soluble fraction (WSF) of the peel samples, uronic acids are the predominant monomers. On the contrary, rhamnogalacturonan-I type neutral sugars, and especially arabinose and galactose, contribute equally, as compared to uronic acid, to the WSF of the pulp samples. Despite the low average DM value of pulp and peel pectin, pectic substances in both samples showed affinity for antibodies with different specificities indicating that a wide range of epitopes, including long blocks of unesterified galacturonic acids (GalA) residues as well as (short) blocks of esterified GalA residues, are present. No large differences between the pectic substances among the different dragon fruit varieties were observed.
Keywords
Hemicellulose, Dragon fruit, Degree of methoxylation, Sugar composition, Anti-pectin antibodies, Pectin, OPUNTIA-FICUS-INDICA, LOW-METHOXYL PECTIN, EXTRACTION CONDITIONS, ANTIOXIDANT ACTIVITY, HOMOGALACTURONAN, BIOSYNTHESIS, CHEMISTRY, FIRMNESS, BEHAVIOR, TISSUES

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Chicago
Liaotrakoon, Wijitra, Sandy Van Buggenhout, Stefanie Christiaens, Ken Houben, Nathalie De Clercq, Koen Dewettinck, and Marc E Hendrickx. 2013. “An Explorative Study on the Cell Wall Polysaccharides in the Pulp and Peel of Dragon Fruits (Hylocereus Spp.).” European Food Research and Technology 237 (3): 341–351.
APA
Liaotrakoon, W., Van Buggenhout, S., Christiaens, S., Houben, K., De Clercq, N., Dewettinck, K., & Hendrickx, M. E. (2013). An explorative study on the cell wall polysaccharides in the pulp and peel of dragon fruits (Hylocereus spp.). EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 237(3), 341–351.
Vancouver
1.
Liaotrakoon W, Van Buggenhout S, Christiaens S, Houben K, De Clercq N, Dewettinck K, et al. An explorative study on the cell wall polysaccharides in the pulp and peel of dragon fruits (Hylocereus spp.). EUROPEAN FOOD RESEARCH AND TECHNOLOGY. 2013;237(3):341–51.
MLA
Liaotrakoon, Wijitra, Sandy Van Buggenhout, Stefanie Christiaens, et al. “An Explorative Study on the Cell Wall Polysaccharides in the Pulp and Peel of Dragon Fruits (Hylocereus Spp.).” EUROPEAN FOOD RESEARCH AND TECHNOLOGY 237.3 (2013): 341–351. Print.
@article{3229338,
  abstract     = {The pectic and hemicellulosic cell wall polysaccharides from the pulp and the peel of white-flesh and red-flesh dragon fruits (Hylocereus spp.) were isolated and compared in terms of degree of methoxylation (DM), solubility properties (relative content of uronic acids and neutral sugars in different fractions), neutral sugar composition, molar mass distribution, and affinity towards some specific anti-pectin antibodies. Hereto, the alcohol insoluble residues were extracted and sequentially fractionated using hot water, a chelating agent, sodium carbonate, and potassium hydroxide solutions to obtain different pectin fractions and a hemicellulose fraction. Chemical analyses were used to characterize these polysaccharide fractions. The results show that cell wall polysaccharides of the pulp and especially of the peel from white-flesh and red-flesh dragon fruits contain significant amounts of pectic substances that are lowly methyl-esterified. The cell wall polysaccharides of the peel as well as those of the pulp contain high amounts (38-47\%) of loosely bound (water-soluble) pectic substances. In the water-soluble fraction (WSF) of the peel samples, uronic acids are the predominant monomers. On the contrary, rhamnogalacturonan-I type neutral sugars, and especially arabinose and galactose, contribute equally, as compared to uronic acid, to the WSF of the pulp samples. Despite the low average DM value of pulp and peel pectin, pectic substances in both samples showed affinity for antibodies with different specificities indicating that a wide range of epitopes, including long blocks of unesterified galacturonic acids (GalA) residues as well as (short) blocks of esterified GalA residues, are present. No large differences between the pectic substances among the different dragon fruit varieties were observed.},
  author       = {Liaotrakoon, Wijitra and Van Buggenhout, Sandy and Christiaens, Stefanie and Houben, Ken and De Clercq, Nathalie and Dewettinck, Koen and Hendrickx, Marc E},
  issn         = {1438-2377},
  journal      = {EUROPEAN FOOD RESEARCH AND TECHNOLOGY},
  language     = {eng},
  number       = {3},
  pages        = {341--351},
  title        = {An explorative study on the cell wall polysaccharides in the pulp and peel of dragon fruits (Hylocereus spp.)},
  url          = {http://dx.doi.org/10.1007/s00217-013-1997-7},
  volume       = {237},
  year         = {2013},
}

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