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Atrial natriuretic peptides detect cardiac dilatation

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Abstract
Background : Atrial natriuretic peptides are released in case of atrial and ventricular stretch and might be useful in diagnosis and prognosis of valvular heart disease in horses. The aim was to compare atrial natriuretic peptide (ANP) and N-terminal proANP (NT-proANP) levels in healthy horses and horses with valvular heart disease and to determine ANP and NTproANP stability in samples stored at -20°C and -80°C. Method : Blood samples were collected from 20 healthy horses (body weight 572±54kg, age 7.9±4.3 years), 21 horses with valvular regurgitation without dilatation (body weight 565±87kg, age 12±7.3 years) and 8 horses with valvular regurgitation associated with atrial or ventricular dilatation (body weight 566±60kg, age 13.7±5.2 years). Samples were centrifuged at 4°C within 30 min after collection and stored at -20°C and -80°C. ANP and NT-proANP concentration were measured using an equine and human ELISA (E90225EQ and E90484Hu, USCN, Wuhan, China), respectively. Ten samples were reanalyzed after 15-60 days. Results : ANP levels were significantly higher in horses with atrial dilatation compared with healthy horses (PANP=0.02; PNTproANP=0.08). No significant difference was seen between horses with valvular regurgitation without atrial or ventricular dilatation and healthy horses (PANP=1.0 and PNTproANP =0.91). A significant decrease in ANP and NT-proANP levels was seen after storage at -20°C (PANP=0.01 and PNTproANP=0.02) and -80°C (PANP=0.01, PNT-proANP=0.03). The decrease in NT-proANP was smaller than in ANP (P=0.08). Conclusion : Chamber dilatation results in ANP and NT-proANP increase and these cardiac biomarkers could be used for clinical purposes. Sample storage time negatively influences (NTpro) ANP levels. Therefore, sample analysis should be done as soon as possible.
Keywords
ANP, natriuretic peptides, NT-proANP, cardiac biomarker, horses

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Chicago
Van Der Vekens, Nicky, Annelies Decloedt, Dominique De Clercq, and Gunther van Loon. 2013. “Atrial Natriuretic Peptides Detect Cardiac Dilatation.” In European Veterinary Conference : Voorjaarsdagen, Abstracts, 258–258.
APA
Van Der Vekens, N., Decloedt, A., De Clercq, D., & van Loon, G. (2013). Atrial natriuretic peptides detect cardiac dilatation. European Veterinary Conference : Voorjaarsdagen, Abstracts (pp. 258–258). Presented at the 2013 European Veterinary Conference : Voorjaarsdagen.
Vancouver
1.
Van Der Vekens N, Decloedt A, De Clercq D, van Loon G. Atrial natriuretic peptides detect cardiac dilatation. European Veterinary Conference : Voorjaarsdagen, Abstracts. 2013. p. 258–258.
MLA
Van Der Vekens, Nicky, Annelies Decloedt, Dominique De Clercq, et al. “Atrial Natriuretic Peptides Detect Cardiac Dilatation.” European Veterinary Conference : Voorjaarsdagen, Abstracts. 2013. 258–258. Print.
@inproceedings{3227757,
  abstract     = {Background : Atrial natriuretic peptides are released in case of atrial and ventricular stretch and might be useful in diagnosis and prognosis of valvular heart disease in horses. The aim was to compare atrial natriuretic peptide (ANP) and N-terminal proANP (NT-proANP) levels in healthy horses and horses with valvular heart disease and to determine ANP and NTproANP stability in samples stored at -20{\textdegree}C and -80{\textdegree}C.
Method : Blood samples were collected from 20 healthy horses (body weight 572{\textpm}54kg, age 7.9{\textpm}4.3 years), 21 horses with valvular regurgitation without dilatation (body weight 565{\textpm}87kg, age 12{\textpm}7.3 years) and 8 horses with valvular regurgitation associated with atrial or ventricular dilatation (body weight 566{\textpm}60kg, age 13.7{\textpm}5.2 years). Samples were centrifuged at 4{\textdegree}C within 30 min after collection and stored at -20{\textdegree}C and -80{\textdegree}C. ANP and NT-proANP concentration were measured using an equine and human ELISA (E90225EQ and E90484Hu, USCN, Wuhan, China), respectively. Ten samples were reanalyzed after 15-60 days.
Results : ANP levels were significantly higher in horses with atrial dilatation compared with healthy horses (PANP=0.02; PNTproANP=0.08). No significant difference was seen between horses with valvular regurgitation without atrial or ventricular dilatation and healthy horses (PANP=1.0 and PNTproANP =0.91). A significant decrease in ANP and NT-proANP levels was seen after storage at -20{\textdegree}C (PANP=0.01 and PNTproANP=0.02) and -80{\textdegree}C (PANP=0.01, PNT-proANP=0.03). The decrease in NT-proANP was smaller than in ANP (P=0.08).
Conclusion : Chamber dilatation results in ANP and NT-proANP increase and these cardiac biomarkers could be used for clinical purposes. Sample storage time negatively influences (NTpro) ANP levels. Therefore, sample analysis should be done as soon as possible.},
  author       = {Van Der Vekens, Nicky and Decloedt, Annelies and De Clercq, Dominique and van Loon, Gunther},
  booktitle    = {European Veterinary Conference : Voorjaarsdagen, Abstracts},
  language     = {eng},
  location     = {Amsterdam, The Netherlands},
  pages        = {258--258},
  title        = {Atrial natriuretic peptides detect cardiac dilatation},
  year         = {2013},
}