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P-cadherin promotes cell-cell adhesion and counteracts invasion in human melanoma

(2005) CANCER RESEARCH. 65(19). p.8774-8783
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Abstract
Malignant transformation of melanocytes frequently coincides with alterations in epithelial cadherin (E-cadherin) expression, switching on of neural cadherin (N-cadherin), and, when progressed to a metastatic stage, loss of membranous placental cadherin (P-cadherin). In vitro studies of melanoma cell lines have shown invasion suppressor and promoter roles for E-cadherin and N-cadherin, respectively. In the present study, we investigated the effect of P-cadherin on aggregation and invasion using melanoma cells retrovirally transduced with human P-cadherin. De novo expression of P-cadherin in P-cadherin-negative cell lines (BLM and HMB2) promoted cell-cell contacts and Ca2+-dependent cell-cell aggregation in two- and three-dimensional cultures, whereas it counteracted invasion. These effects were not observed following P-cadherin transduction of endogenously P-cadherin-positive MeWo cells. In addition, P-cadherin-transduced BLM cells coaggregated with keratinocytes and showed markedly reduced invasion in a reconstructed skin model. The proadhesive and anti-invasive effects of P-cadherin were abolished on targeted mutation of its intracellular juxtamembrane domain or its extracellular domain. For the latter mutation, we mimicked a known missense mutation in P-cadherin (R503H), which is associated with congenital hypotrichosis with juvenile macular dystrophy.
Keywords
BREAST-CANCER CELLS, JUVENILE MACULAR DYSTROPHY, N-CADHERIN, BETA-CATENIN, P120 CATENIN, IN-VITRO, EXPRESSION, TUMOR, PROGRESSION, HYPOTRICHOSIS

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Chicago
Van Marck, Veerle, Christophe Stove, Karolien Van Den Bossche, Veronique Stove, Joana Paredes, Yves Vander Haeghen, and Marc Bracke. 2005. “P-cadherin Promotes Cell-cell Adhesion and Counteracts Invasion in Human Melanoma.” Cancer Research 65 (19): 8774–8783.
APA
Van Marck, V., Stove, C., Van Den Bossche, K., Stove, V., Paredes, J., Vander Haeghen, Y., & Bracke, M. (2005). P-cadherin promotes cell-cell adhesion and counteracts invasion in human melanoma. CANCER RESEARCH, 65(19), 8774–8783.
Vancouver
1.
Van Marck V, Stove C, Van Den Bossche K, Stove V, Paredes J, Vander Haeghen Y, et al. P-cadherin promotes cell-cell adhesion and counteracts invasion in human melanoma. CANCER RESEARCH. 2005;65(19):8774–83.
MLA
Van Marck, Veerle, Christophe Stove, Karolien Van Den Bossche, et al. “P-cadherin Promotes Cell-cell Adhesion and Counteracts Invasion in Human Melanoma.” CANCER RESEARCH 65.19 (2005): 8774–8783. Print.
@article{322739,
  abstract     = {Malignant transformation of melanocytes frequently coincides with alterations in epithelial cadherin (E-cadherin) expression, switching on of neural cadherin (N-cadherin), and, when progressed to a metastatic stage, loss of membranous placental cadherin (P-cadherin). In vitro studies of melanoma cell lines have shown invasion suppressor and promoter roles for E-cadherin and N-cadherin, respectively. In the present study, we investigated the effect of P-cadherin on aggregation and invasion using melanoma cells retrovirally transduced with human P-cadherin. De novo expression of P-cadherin in P-cadherin-negative cell lines (BLM and HMB2) promoted cell-cell contacts and Ca2+-dependent cell-cell aggregation in two- and three-dimensional cultures, whereas it counteracted invasion. These effects were not observed following P-cadherin transduction of endogenously P-cadherin-positive MeWo cells. In addition, P-cadherin-transduced BLM cells coaggregated with keratinocytes and showed markedly reduced invasion in a reconstructed skin model. The proadhesive and anti-invasive effects of P-cadherin were abolished on targeted mutation of its intracellular juxtamembrane domain or its extracellular domain. For the latter mutation, we mimicked a known missense mutation in P-cadherin (R503H), which is associated with congenital hypotrichosis with juvenile macular dystrophy.},
  author       = {Van Marck, Veerle and Stove, Christophe and Van Den Bossche, Karolien and Stove, Veronique and Paredes, Joana and Vander Haeghen, Yves and Bracke, Marc},
  issn         = {0008-5472},
  journal      = {CANCER RESEARCH},
  keywords     = {BREAST-CANCER CELLS,JUVENILE MACULAR DYSTROPHY,N-CADHERIN,BETA-CATENIN,P120 CATENIN,IN-VITRO,EXPRESSION,TUMOR,PROGRESSION,HYPOTRICHOSIS},
  language     = {eng},
  number       = {19},
  pages        = {8774--8783},
  title        = {P-cadherin promotes cell-cell adhesion and counteracts invasion in human melanoma},
  url          = {http://dx.doi.org/10.1158/0008-5472.CAN-04-4414},
  volume       = {65},
  year         = {2005},
}

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