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Single-photon emission computed tomographic imaging of the early time course of therapy-induced cell death using technetium 99m tricarbonyl His-annexin A5 in a colorectal cancer xenograft model

(2012) MOLECULAR IMAGING. 11(2). p.135-147
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Abstract
As apoptosis occurs over an interval of time after administration of apoptosis-inducing therapy in tumors, the changes in technetium 99m (Tc-99m)-tricarbonyl (CO)(3) His-annexin A5 (His-ann A5) accumulation over time were examined. Colo205-bearing mice were divided into six treatment groups: (1) control, (2) 5-fluorouracil (5-FU; 250 mg/kg), (3) irinotecan (100 mg/kg), (4) oxaliplatin (30 mg/kg), (5) bevacizumab (5 mg/kg), and (6) panitumumab (6 mg/kg). (99)mTc-(CO)(3) His-ann A5 was injected 4, 8, 12, 24, and 48 hours posttreatment, and micro-single-photon emission computed tomography was performed. Immunostaining of caspase-3 (apoptosis), survivin (antiapoptosis), and LC3-II (autophagy marker) was also performed. Different dynamics of (99)mTc-(CO)(3) His-ann A5 uptake were observed in this colorectal cancer xenograft model, in response to a single dose of three different chemotherapeutics (5-FU, irinotecan, and oxaliplatin). Bevacizumab-treated mice showed no increased uptake of the radiotracer, and a peak of (99)mTc-(CO)(3) HisannA5 uptake in panitumumab-treated mice was observed 24 hours posttreatment, as confirmed by caspase-3 immunostaining. For irinotecan-, oxaliplatin-, and bevacizumab-treated tumors, a significant correlation was established between the radiotracer uptake and caspase-3 immunostaining (r = .8, p < .05; r = .9, p < .001; r = .9, p < .001, respectively). For 5-FU- and panitumumab-treated mice, the correlation coefficients were r = .7 (p = .18) and r = .7 (p = .19), respectively. Optimal timing of annexin A5 imaging after the start of different treatments in the Colo205 model was determined.
Keywords
EFFICACY, INCREASES, PANITUMUMAB, PHOSPHATIDYLSERINE, AUTOPHAGY, CHEMOTHERAPY, APOPTOTIC TUMOR RESPONSE, IN-VIVO DETECTION, IONIZING-RADIATION, V SCINTIGRAPHY

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MLA
VANGESTEL, CHRISTEL, et al. “Single-Photon Emission Computed Tomographic Imaging of the Early Time Course of Therapy-Induced Cell Death Using Technetium 99m Tricarbonyl His-Annexin A5 in a Colorectal Cancer Xenograft Model.” MOLECULAR IMAGING, vol. 11, no. 2, 2012, pp. 135–47, doi:10.2310/7290.2011.00034.
APA
VANGESTEL, C., Van De Wiele, C., Mees, G., Mertens, K., Staelens, S., Reutelingsperger, C., … Peeters, M. (2012). Single-photon emission computed tomographic imaging of the early time course of therapy-induced cell death using technetium 99m tricarbonyl His-annexin A5 in a colorectal cancer xenograft model. MOLECULAR IMAGING, 11(2), 135–147. https://doi.org/10.2310/7290.2011.00034
Chicago author-date
VANGESTEL, CHRISTEL, Christophe Van De Wiele, Gilles Mees, Koen Mertens, Steven Staelens, Chris Reutelingsperger, Patrick Pauwels, Nancy Van Damme, and Marc Peeters. 2012. “Single-Photon Emission Computed Tomographic Imaging of the Early Time Course of Therapy-Induced Cell Death Using Technetium 99m Tricarbonyl His-Annexin A5 in a Colorectal Cancer Xenograft Model.” MOLECULAR IMAGING 11 (2): 135–47. https://doi.org/10.2310/7290.2011.00034.
Chicago author-date (all authors)
VANGESTEL, CHRISTEL, Christophe Van De Wiele, Gilles Mees, Koen Mertens, Steven Staelens, Chris Reutelingsperger, Patrick Pauwels, Nancy Van Damme, and Marc Peeters. 2012. “Single-Photon Emission Computed Tomographic Imaging of the Early Time Course of Therapy-Induced Cell Death Using Technetium 99m Tricarbonyl His-Annexin A5 in a Colorectal Cancer Xenograft Model.” MOLECULAR IMAGING 11 (2): 135–147. doi:10.2310/7290.2011.00034.
Vancouver
1.
VANGESTEL C, Van De Wiele C, Mees G, Mertens K, Staelens S, Reutelingsperger C, et al. Single-photon emission computed tomographic imaging of the early time course of therapy-induced cell death using technetium 99m tricarbonyl His-annexin A5 in a colorectal cancer xenograft model. MOLECULAR IMAGING. 2012;11(2):135–47.
IEEE
[1]
C. VANGESTEL et al., “Single-photon emission computed tomographic imaging of the early time course of therapy-induced cell death using technetium 99m tricarbonyl His-annexin A5 in a colorectal cancer xenograft model,” MOLECULAR IMAGING, vol. 11, no. 2, pp. 135–147, 2012.
@article{3226976,
  abstract     = {{As apoptosis occurs over an interval of time after administration of apoptosis-inducing therapy in tumors, the changes in technetium 99m (Tc-99m)-tricarbonyl (CO)(3) His-annexin A5 (His-ann A5) accumulation over time were examined. Colo205-bearing mice were divided into six treatment groups: (1) control, (2) 5-fluorouracil (5-FU; 250 mg/kg), (3) irinotecan (100 mg/kg), (4) oxaliplatin (30 mg/kg), (5) bevacizumab (5 mg/kg), and (6) panitumumab (6 mg/kg). (99)mTc-(CO)(3) His-ann A5 was injected 4, 8, 12, 24, and 48 hours posttreatment, and micro-single-photon emission computed tomography was performed. Immunostaining of caspase-3 (apoptosis), survivin (antiapoptosis), and LC3-II (autophagy marker) was also performed. Different dynamics of (99)mTc-(CO)(3) His-ann A5 uptake were observed in this colorectal cancer xenograft model, in response to a single dose of three different chemotherapeutics (5-FU, irinotecan, and oxaliplatin). Bevacizumab-treated mice showed no increased uptake of the radiotracer, and a peak of (99)mTc-(CO)(3) HisannA5 uptake in panitumumab-treated mice was observed 24 hours posttreatment, as confirmed by caspase-3 immunostaining. For irinotecan-, oxaliplatin-, and bevacizumab-treated tumors, a significant correlation was established between the radiotracer uptake and caspase-3 immunostaining (r = .8, p < .05; r = .9, p < .001; r = .9, p < .001, respectively). For 5-FU- and panitumumab-treated mice, the correlation coefficients were r = .7 (p = .18) and r = .7 (p = .19), respectively. Optimal timing of annexin A5 imaging after the start of different treatments in the Colo205 model was determined.}},
  author       = {{VANGESTEL, CHRISTEL and Van De Wiele, Christophe and Mees, Gilles and Mertens, Koen and Staelens, Steven and Reutelingsperger, Chris and Pauwels, Patrick and Van Damme, Nancy and Peeters, Marc}},
  issn         = {{1535-3508}},
  journal      = {{MOLECULAR IMAGING}},
  keywords     = {{EFFICACY,INCREASES,PANITUMUMAB,PHOSPHATIDYLSERINE,AUTOPHAGY,CHEMOTHERAPY,APOPTOTIC TUMOR RESPONSE,IN-VIVO DETECTION,IONIZING-RADIATION,V SCINTIGRAPHY}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{135--147}},
  title        = {{Single-photon emission computed tomographic imaging of the early time course of therapy-induced cell death using technetium 99m tricarbonyl His-annexin A5 in a colorectal cancer xenograft model}},
  url          = {{http://dx.doi.org/10.2310/7290.2011.00034}},
  volume       = {{11}},
  year         = {{2012}},
}

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