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Polymer-coated nanoparticles interacting with proteins and cells: focusing on the sign of the net charge

Dominik Hühn, Karsten Kantner, Christian Geidel, Stefan Brandholt, Ine De Cock (UGent) , Stefaan Soenen (UGent) , Pilar Rivera Gil, Jose Maria Montenegro, Kevin Braeckmans (UGent) , Klaus Müllen, et al.
(2013) ACS NANO. 7(4). p.3253-3263
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Abstract
To study charge-dependent interactions of nanopartides (NPs) with biological media and NP uptake by cells, colloidal gold nanopanicles were modified with amphiphilic polymers to obtain NPs with identical physical properties except for the sign of the charge (negative/positive). This strategy enabled us to solely assess the influence of charge on the interactions of the NPs with proteins and cells, without interference by other effects such as different size and colloidal stability. Our study shows that the number of adsorbed human serum albumin molecules per NP was not influenced by their surface charge. Positively charged NPs were incorporated by cells to a larger extent than negatively charged ones, both in serum-free and serum-containing media. Consequently, with and without protein corona (i.e., in serum-free medium) present, NP internalization depends on the sign of charge. The uptake rate of NPs by cells was higher for positively than for negatively charged NPs. Furthermore, cytotoxicity assays revealed a higher cytotoxicity for positively charged NPs, associated with their enhanced uptake.
Keywords
surface charge dependence, colloidal gold nanoparticles, protein corona, uptake by cells, cytotoxicity, FLUORESCENCE CORRELATION SPECTROSCOPY, IRON-OXIDE NANOPARTICLES, GOLD NANOPARTICLES, SURFACE-CHARGE, CELLULAR UPTAKE, INORGANIC NANOPARTICLES, COLLOIDAL NANOPARTICLES, QUANTUM DOTS, HELA-CELLS, CORONA

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MLA
Hühn, Dominik, et al. “Polymer-Coated Nanoparticles Interacting with Proteins and Cells: Focusing on the Sign of the Net Charge.” ACS NANO, vol. 7, no. 4, 2013, pp. 3253–63, doi:10.1021/nn3059295.
APA
Hühn, D., Kantner, K., Geidel, C., Brandholt, S., De Cock, I., Soenen, S., … Parak, W. J. (2013). Polymer-coated nanoparticles interacting with proteins and cells: focusing on the sign of the net charge. ACS NANO, 7(4), 3253–3263. https://doi.org/10.1021/nn3059295
Chicago author-date
Hühn, Dominik, Karsten Kantner, Christian Geidel, Stefan Brandholt, Ine De Cock, Stefaan Soenen, Pilar Rivera Gil, et al. 2013. “Polymer-Coated Nanoparticles Interacting with Proteins and Cells: Focusing on the Sign of the Net Charge.” ACS NANO 7 (4): 3253–63. https://doi.org/10.1021/nn3059295.
Chicago author-date (all authors)
Hühn, Dominik, Karsten Kantner, Christian Geidel, Stefan Brandholt, Ine De Cock, Stefaan Soenen, Pilar Rivera Gil, Jose Maria Montenegro, Kevin Braeckmans, Klaus Müllen, G Ulrich Nienhaus, Markus Klapper, and Wolfgang J Parak. 2013. “Polymer-Coated Nanoparticles Interacting with Proteins and Cells: Focusing on the Sign of the Net Charge.” ACS NANO 7 (4): 3253–3263. doi:10.1021/nn3059295.
Vancouver
1.
Hühn D, Kantner K, Geidel C, Brandholt S, De Cock I, Soenen S, et al. Polymer-coated nanoparticles interacting with proteins and cells: focusing on the sign of the net charge. ACS NANO. 2013;7(4):3253–63.
IEEE
[1]
D. Hühn et al., “Polymer-coated nanoparticles interacting with proteins and cells: focusing on the sign of the net charge,” ACS NANO, vol. 7, no. 4, pp. 3253–3263, 2013.
@article{3226299,
  abstract     = {{To study charge-dependent interactions of nanopartides (NPs) with biological media and NP uptake by cells, colloidal gold nanopanicles were modified with amphiphilic polymers to obtain NPs with identical physical properties except for the sign of the charge (negative/positive). This strategy enabled us to solely assess the influence of charge on the interactions of the NPs with proteins and cells, without interference by other effects such as different size and colloidal stability. Our study shows that the number of adsorbed human serum albumin molecules per NP was not influenced by their surface charge. Positively charged NPs were incorporated by cells to a larger extent than negatively charged ones, both in serum-free and serum-containing media. Consequently, with and without protein corona (i.e., in serum-free medium) present, NP internalization depends on the sign of charge. The uptake rate of NPs by cells was higher for positively than for negatively charged NPs. Furthermore, cytotoxicity assays revealed a higher cytotoxicity for positively charged NPs, associated with their enhanced uptake.}},
  author       = {{Hühn, Dominik and Kantner, Karsten and Geidel, Christian and Brandholt, Stefan and De Cock, Ine and Soenen, Stefaan and Rivera Gil, Pilar and Montenegro, Jose Maria and Braeckmans, Kevin and Müllen, Klaus and Nienhaus, G Ulrich and Klapper, Markus and Parak, Wolfgang J}},
  issn         = {{1936-0851}},
  journal      = {{ACS NANO}},
  keywords     = {{surface charge dependence,colloidal gold nanoparticles,protein corona,uptake by cells,cytotoxicity,FLUORESCENCE CORRELATION SPECTROSCOPY,IRON-OXIDE NANOPARTICLES,GOLD NANOPARTICLES,SURFACE-CHARGE,CELLULAR UPTAKE,INORGANIC NANOPARTICLES,COLLOIDAL NANOPARTICLES,QUANTUM DOTS,HELA-CELLS,CORONA}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{3253--3263}},
  title        = {{Polymer-coated nanoparticles interacting with proteins and cells: focusing on the sign of the net charge}},
  url          = {{http://doi.org/10.1021/nn3059295}},
  volume       = {{7}},
  year         = {{2013}},
}
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