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Restriction analysis of an amplified rodA gene fragment to distinguish Aspergillus fumigatus var. ellipticus from Aspergillus fumigatus var. fumigatus

(2012) FEMS MICROBIOLOGY LETTERS. 333(2). p.153-159
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Abstract
A previous multidisciplinary study indicated that gliotoxin-producing Aspergillus fumigatus Fresen. isolates from silage commodities mostly belonged to its variant A. fumigatus var. ellipticus Raper & Fennell. Sequence analysis revealed the presence of a single nucleotide polymorphism at five positions in a fragment of the rodA gene (coding for a hydrophobin rodletA protein) between Aspergillus fumigatus var. fumigatus and Aspergillus fumigatus var. ellipticus. A method was developed to distinguish these two types of isolates based on restriction analysis of this rodA gene fragment using the HinfI restriction enzyme. In addition, in silico analysis of 113 rodA gene fragments retrieved from GenBank was performed and confirmed the suitability of this method. In conclusion, the method developed in this study allows easy distinction between A. fumigatus var. fumigatus and its variant ellipticus. In combination with the earlier developed PCR-restriction fragment length polymorphism method of Staab et al. (2009, J Clin Microbiol 47: 2079), this method is part of a sequencing-independent identification scheme that allows for rapid distinction between similar species/variants within Aspergillus section Fumigati, specifically A. fumigatus, A. fumigatus var. ellipticus, Aspergillus lentulus Balajee & K.A. Marr, Neosartorya pseudofischeri S.W. Peterson and Neosartorya udagawae Y. Horie, Miyaji & Nishim.
Keywords
Aspergillus fumigatus, rodA gene, restriction analysis, SECTION FUMIGATI, NEOSARTORYA-PSEUDOFISCHERI, GLIOTOXIN PRODUCTION, POLYPHASIC TAXONOMY, SUSCEPTIBILITY, IDENTIFICATION, STRAINS, SILAGE, DNA

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Citation

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Chicago
Van Pamel, Els, Els Daeseleire, Nikki De Clercq, Lieve Herman, Annemieke Verbeken, Marc Heyndrickx, and Geertrui Vlaemynck. 2012. “Restriction Analysis of an Amplified rodA Gene Fragment to Distinguish Aspergillus Fumigatus Var. Ellipticus from Aspergillus Fumigatus Var. Fumigatus.” Fems Microbiology Letters 333 (2): 153–159.
APA
Van Pamel, E., Daeseleire, E., De Clercq, N., Herman, L., Verbeken, A., Heyndrickx, M., & Vlaemynck, G. (2012). Restriction analysis of an amplified rodA gene fragment to distinguish Aspergillus fumigatus var. ellipticus from Aspergillus fumigatus var. fumigatus. FEMS MICROBIOLOGY LETTERS, 333(2), 153–159.
Vancouver
1.
Van Pamel E, Daeseleire E, De Clercq N, Herman L, Verbeken A, Heyndrickx M, et al. Restriction analysis of an amplified rodA gene fragment to distinguish Aspergillus fumigatus var. ellipticus from Aspergillus fumigatus var. fumigatus. FEMS MICROBIOLOGY LETTERS. 2012;333(2):153–9.
MLA
Van Pamel, Els, Els Daeseleire, Nikki De Clercq, et al. “Restriction Analysis of an Amplified rodA Gene Fragment to Distinguish Aspergillus Fumigatus Var. Ellipticus from Aspergillus Fumigatus Var. Fumigatus.” FEMS MICROBIOLOGY LETTERS 333.2 (2012): 153–159. Print.
@article{3224175,
  abstract     = {A previous multidisciplinary study indicated that gliotoxin-producing Aspergillus fumigatus Fresen. isolates from silage commodities mostly belonged to its variant A. fumigatus var. ellipticus Raper \& Fennell. Sequence analysis revealed the presence of a single nucleotide polymorphism at five positions in a fragment of the rodA gene (coding for a hydrophobin rodletA protein) between Aspergillus fumigatus var. fumigatus and Aspergillus fumigatus var. ellipticus. A method was developed to distinguish these two types of isolates based on restriction analysis of this rodA gene fragment using the HinfI restriction enzyme. In addition, in silico analysis of 113 rodA gene fragments retrieved from GenBank was performed and confirmed the suitability of this method. In conclusion, the method developed in this study allows easy distinction between A. fumigatus var. fumigatus and its variant ellipticus. In combination with the earlier developed PCR-restriction fragment length polymorphism method of Staab et al. (2009, J Clin Microbiol 47: 2079), this method is part of a sequencing-independent identification scheme that allows for rapid distinction between similar species/variants within Aspergillus section Fumigati, specifically A. fumigatus, A. fumigatus var. ellipticus, Aspergillus lentulus Balajee \& K.A. Marr, Neosartorya pseudofischeri S.W. Peterson and Neosartorya udagawae Y. Horie, Miyaji \& Nishim.},
  author       = {Van Pamel, Els and Daeseleire, Els and De Clercq, Nikki and Herman, Lieve and Verbeken, Annemieke and Heyndrickx, Marc and Vlaemynck, Geertrui},
  issn         = {0378-1097},
  journal      = {FEMS MICROBIOLOGY LETTERS},
  keyword      = {Aspergillus fumigatus,rodA gene,restriction analysis,SECTION FUMIGATI,NEOSARTORYA-PSEUDOFISCHERI,GLIOTOXIN PRODUCTION,POLYPHASIC TAXONOMY,SUSCEPTIBILITY,IDENTIFICATION,STRAINS,SILAGE,DNA},
  language     = {eng},
  number       = {2},
  pages        = {153--159},
  title        = {Restriction analysis of an amplified rodA gene fragment to distinguish Aspergillus fumigatus var. ellipticus from Aspergillus fumigatus var. fumigatus},
  url          = {http://dx.doi.org/10.1111/j.1574-6968.2012.02608.x},
  volume       = {333},
  year         = {2012},
}

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