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Restriction analysis of an amplified rodA gene fragment to distinguish Aspergillus fumigatus var. ellipticus from Aspergillus fumigatus var. fumigatus

Els Van Pamel, Els Daeseleire, Nikki De Clercq, Lieve Herman, Annemieke Verbeken UGent, Marc Heyndrickx UGent and Geertrui Vlaemynck (2012) FEMS MICROBIOLOGY LETTERS. 333(2). p.153-159
abstract
A previous multidisciplinary study indicated that gliotoxin-producing Aspergillus fumigatus Fresen. isolates from silage commodities mostly belonged to its variant A. fumigatus var. ellipticus Raper & Fennell. Sequence analysis revealed the presence of a single nucleotide polymorphism at five positions in a fragment of the rodA gene (coding for a hydrophobin rodletA protein) between Aspergillus fumigatus var. fumigatus and Aspergillus fumigatus var. ellipticus. A method was developed to distinguish these two types of isolates based on restriction analysis of this rodA gene fragment using the HinfI restriction enzyme. In addition, in silico analysis of 113 rodA gene fragments retrieved from GenBank was performed and confirmed the suitability of this method. In conclusion, the method developed in this study allows easy distinction between A. fumigatus var. fumigatus and its variant ellipticus. In combination with the earlier developed PCR-restriction fragment length polymorphism method of Staab et al. (2009, J Clin Microbiol 47: 2079), this method is part of a sequencing-independent identification scheme that allows for rapid distinction between similar species/variants within Aspergillus section Fumigati, specifically A. fumigatus, A. fumigatus var. ellipticus, Aspergillus lentulus Balajee & K.A. Marr, Neosartorya pseudofischeri S.W. Peterson and Neosartorya udagawae Y. Horie, Miyaji & Nishim.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (letterNote)
publication status
published
subject
keyword
Aspergillus fumigatus, rodA gene, restriction analysis, SECTION FUMIGATI, NEOSARTORYA-PSEUDOFISCHERI, GLIOTOXIN PRODUCTION, POLYPHASIC TAXONOMY, SUSCEPTIBILITY, IDENTIFICATION, STRAINS, SILAGE, DNA
journal title
FEMS MICROBIOLOGY LETTERS
FEMS Microbiol. Lett.
volume
333
issue
2
pages
153 - 159
Web of Science type
Letter
Web of Science id
000306735800009
JCR category
MICROBIOLOGY
JCR impact factor
2.049 (2012)
JCR rank
71/116 (2012)
JCR quartile
3 (2012)
ISSN
0378-1097
DOI
10.1111/j.1574-6968.2012.02608.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
3224175
handle
http://hdl.handle.net/1854/LU-3224175
date created
2013-05-24 15:54:03
date last changed
2016-12-19 15:37:52
@article{3224175,
  abstract     = {A previous multidisciplinary study indicated that gliotoxin-producing Aspergillus fumigatus Fresen. isolates from silage commodities mostly belonged to its variant A. fumigatus var. ellipticus Raper \& Fennell. Sequence analysis revealed the presence of a single nucleotide polymorphism at five positions in a fragment of the rodA gene (coding for a hydrophobin rodletA protein) between Aspergillus fumigatus var. fumigatus and Aspergillus fumigatus var. ellipticus. A method was developed to distinguish these two types of isolates based on restriction analysis of this rodA gene fragment using the HinfI restriction enzyme. In addition, in silico analysis of 113 rodA gene fragments retrieved from GenBank was performed and confirmed the suitability of this method. In conclusion, the method developed in this study allows easy distinction between A. fumigatus var. fumigatus and its variant ellipticus. In combination with the earlier developed PCR-restriction fragment length polymorphism method of Staab et al. (2009, J Clin Microbiol 47: 2079), this method is part of a sequencing-independent identification scheme that allows for rapid distinction between similar species/variants within Aspergillus section Fumigati, specifically A. fumigatus, A. fumigatus var. ellipticus, Aspergillus lentulus Balajee \& K.A. Marr, Neosartorya pseudofischeri S.W. Peterson and Neosartorya udagawae Y. Horie, Miyaji \& Nishim.},
  author       = {Van Pamel, Els and Daeseleire, Els and De Clercq, Nikki and Herman, Lieve and Verbeken, Annemieke and Heyndrickx, Marc and Vlaemynck, Geertrui},
  issn         = {0378-1097},
  journal      = {FEMS MICROBIOLOGY LETTERS},
  keyword      = {Aspergillus fumigatus,rodA gene,restriction analysis,SECTION FUMIGATI,NEOSARTORYA-PSEUDOFISCHERI,GLIOTOXIN PRODUCTION,POLYPHASIC TAXONOMY,SUSCEPTIBILITY,IDENTIFICATION,STRAINS,SILAGE,DNA},
  language     = {eng},
  number       = {2},
  pages        = {153--159},
  title        = {Restriction analysis of an amplified rodA gene fragment to distinguish Aspergillus fumigatus var. ellipticus from Aspergillus fumigatus var. fumigatus},
  url          = {http://dx.doi.org/10.1111/j.1574-6968.2012.02608.x},
  volume       = {333},
  year         = {2012},
}

Chicago
Van Pamel, Els, Els Daeseleire, Nikki De Clercq, Lieve Herman, Annemieke Verbeken, Marc Heyndrickx, and Geertrui Vlaemynck. 2012. “Restriction Analysis of an Amplified rodA Gene Fragment to Distinguish Aspergillus Fumigatus Var. Ellipticus from Aspergillus Fumigatus Var. Fumigatus.” Fems Microbiology Letters 333 (2): 153–159.
APA
Van Pamel, E., Daeseleire, E., De Clercq, N., Herman, L., Verbeken, A., Heyndrickx, M., & Vlaemynck, G. (2012). Restriction analysis of an amplified rodA gene fragment to distinguish Aspergillus fumigatus var. ellipticus from Aspergillus fumigatus var. fumigatus. FEMS MICROBIOLOGY LETTERS, 333(2), 153–159.
Vancouver
1.
Van Pamel E, Daeseleire E, De Clercq N, Herman L, Verbeken A, Heyndrickx M, et al. Restriction analysis of an amplified rodA gene fragment to distinguish Aspergillus fumigatus var. ellipticus from Aspergillus fumigatus var. fumigatus. FEMS MICROBIOLOGY LETTERS. 2012;333(2):153–9.
MLA
Van Pamel, Els, Els Daeseleire, Nikki De Clercq, et al. “Restriction Analysis of an Amplified rodA Gene Fragment to Distinguish Aspergillus Fumigatus Var. Ellipticus from Aspergillus Fumigatus Var. Fumigatus.” FEMS MICROBIOLOGY LETTERS 333.2 (2012): 153–159. Print.