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Linkage mapping identifies the sex determining region as a single locus in the pennate diatom Seminavis robusta

Ives Vanstechelman UGent, Koen Sabbe UGent, Wim Vyverman UGent, Pieter Vanormelingen and Marnik Vuylsteke UGent (2013) PLOS ONE. 8(3).
abstract
The pennate diatom Seminavis robusta, characterized by an archetypical diatom life cycle including a heterothallic mating system, is emerging as a model system for studying the molecular regulation of the diatom cell and life cycle. One of its main advantages compared with other diatom model systems is that sexual crosses can be made routinely, offering unprecedented possibilities for forward genetics. To date, nothing is known about the genetic basis of sex determination in diatoms. Here, we report on the construction of mating type-specific linkage maps for S. robusta, and use them to identify a single locus sex determination system in this diatom. We identified 13 mating type plus and 15 mating type minus linkage groups obtained from the analysis of 463 AFLP markers segregating in a full-sib family, covering 963.7 and 972.2 cM, respectively. Five linkage group pairs could be identified as putative homologues. The mating type phenotype mapped as a monogenic trait, disclosing the mating type plus as the heterogametic sex. This study provides the first evidence for a genetic sex determining mechanism in a diatom.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
EVOLUTION, SEQUENCE, AFLP, BACILLARIOPHYCEAE, MAP, REPRODUCTION, PHYTOPHTHORA-INFESTANS, GENOME, AVIRULENCE GENES, CLONES
journal title
PLOS ONE
PLoS One
volume
8
issue
3
article number
e60132
pages
9 pages
Web of Science type
Article
Web of Science id
000317562600119
JCR category
MULTIDISCIPLINARY SCIENCES
JCR impact factor
3.534 (2013)
JCR rank
8/55 (2013)
JCR quartile
1 (2013)
ISSN
1932-6203
DOI
10.1371/journal.pone.0060132
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
3223138
handle
http://hdl.handle.net/1854/LU-3223138
date created
2013-05-23 16:53:52
date last changed
2016-12-21 15:42:49
@article{3223138,
  abstract     = {The pennate diatom Seminavis robusta, characterized by an archetypical diatom life cycle including a heterothallic mating system, is emerging as a model system for studying the molecular regulation of the diatom cell and life cycle. One of its main advantages compared with other diatom model systems is that sexual crosses can be made routinely, offering unprecedented possibilities for forward genetics. To date, nothing is known about the genetic basis of sex determination in diatoms. Here, we report on the construction of mating type-specific linkage maps for S. robusta, and use them to identify a single locus sex determination system in this diatom. We identified 13 mating type plus and 15 mating type minus linkage groups obtained from the analysis of 463 AFLP markers segregating in a full-sib family, covering 963.7 and 972.2 cM, respectively. Five linkage group pairs could be identified as putative homologues. The mating type phenotype mapped as a monogenic trait, disclosing the mating type plus as the heterogametic sex. This study provides the first evidence for a genetic sex determining mechanism in a diatom.},
  articleno    = {e60132},
  author       = {Vanstechelman, Ives and Sabbe, Koen and Vyverman, Wim and Vanormelingen, Pieter and Vuylsteke, Marnik},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {EVOLUTION,SEQUENCE,AFLP,BACILLARIOPHYCEAE,MAP,REPRODUCTION,PHYTOPHTHORA-INFESTANS,GENOME,AVIRULENCE GENES,CLONES},
  language     = {eng},
  number       = {3},
  pages        = {9},
  title        = {Linkage mapping identifies the sex determining region as a single locus in the pennate diatom Seminavis robusta},
  url          = {http://dx.doi.org/10.1371/journal.pone.0060132},
  volume       = {8},
  year         = {2013},
}

Chicago
Vanstechelman, Ives, Koen Sabbe, Wim Vyverman, Pieter Vanormelingen, and Marnik Vuylsteke. 2013. “Linkage Mapping Identifies the Sex Determining Region as a Single Locus in the Pennate Diatom Seminavis Robusta.” Plos One 8 (3).
APA
Vanstechelman, I., Sabbe, K., Vyverman, W., Vanormelingen, P., & Vuylsteke, M. (2013). Linkage mapping identifies the sex determining region as a single locus in the pennate diatom Seminavis robusta. PLOS ONE, 8(3).
Vancouver
1.
Vanstechelman I, Sabbe K, Vyverman W, Vanormelingen P, Vuylsteke M. Linkage mapping identifies the sex determining region as a single locus in the pennate diatom Seminavis robusta. PLOS ONE. 2013;8(3).
MLA
Vanstechelman, Ives, Koen Sabbe, Wim Vyverman, et al. “Linkage Mapping Identifies the Sex Determining Region as a Single Locus in the Pennate Diatom Seminavis Robusta.” PLOS ONE 8.3 (2013): n. pag. Print.