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Cell survival and proliferation after encapsulation in a chemically modified Pluronic® F127 hydrogel

Evi Lippens (UGent) , Ives Swennen (UGent) , Jordi Gironès Molera (UGent) , Heidi Declercq (UGent) , Geert Vertenten (UGent) , Lieven Vlaminck (UGent) , Frank Gasthuys (UGent) , Etienne Schacht (UGent) and Maria Cornelissen (UGent)
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Abstract
Pluronic((R)) F127 is a biocompatible, injectable, and thermoresponsive polymer with promising biomedical applications. In this study, a chemically modified form, i.e., Pluronic ALA-L with tailored degradation rate, was tested as an encapsulation vehicle for osteoblastic cells. UV cross-linking of the modified polymer results in a stable hydrogel with a slower degradation rate. Toxicological screening showed no adverse effects of the modified Pluronic ALA-L on the cell viability. Moreover, high viability of embedded cells in the cross-linked Pluronic ALA-L was observed with life/death fluorescent staining during a 7-day-culture period. Cells were also cultured on macroporous, cross-linked gelatin microbeads, called CultiSpher-S-(R) carriers, and encapsulated into the modified cross-linked hydrogel. Also, in this situation, good cell proliferation and migration could be observed in vitro. Preliminary in vivo tests have shown the formation of new bone starting from the injected pre-loaded CultiSpher-S-(R) carriers.
Keywords
BONE, MICROCARRIERS, IN-VITRO, STEM-CELLS, DRUG-DELIVERY, BIOMEDICAL APPLICATIONS, THERMORESPONSIVE HYDROGELS, PHARMACEUTICAL FORMULATIONS, bone formation, Pluronic, cell encapsulation, hydrogel, SCAFFOLDS, CultiSpher, CARTILAGE

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Chicago
Lippens, Evi, Ives Swennen, Jordi Gironès Molera, Heidi Declercq, Geert Vertenten, Lieven Vlaminck, Frank Gasthuys, Etienne Schacht, and Maria Cornelissen. 2013. “Cell Survival and Proliferation After Encapsulation in a Chemically Modified Pluronic® F127 Hydrogel.” Journal of Biomaterials Applications 27 (7): 828–839.
APA
Lippens, E., Swennen, I., Gironès Molera, J., Declercq, H., Vertenten, G., Vlaminck, L., Gasthuys, F., et al. (2013). Cell survival and proliferation after encapsulation in a chemically modified Pluronic® F127 hydrogel. JOURNAL OF BIOMATERIALS APPLICATIONS, 27(7), 828–839.
Vancouver
1.
Lippens E, Swennen I, Gironès Molera J, Declercq H, Vertenten G, Vlaminck L, et al. Cell survival and proliferation after encapsulation in a chemically modified Pluronic® F127 hydrogel. JOURNAL OF BIOMATERIALS APPLICATIONS. 2013;27(7):828–39.
MLA
Lippens, Evi, Ives Swennen, Jordi Gironès Molera, et al. “Cell Survival and Proliferation After Encapsulation in a Chemically Modified Pluronic® F127 Hydrogel.” JOURNAL OF BIOMATERIALS APPLICATIONS 27.7 (2013): 828–839. Print.
@article{3219732,
  abstract     = {Pluronic((R)) F127 is a biocompatible, injectable, and thermoresponsive polymer with promising biomedical applications. In this study, a chemically modified form, i.e., Pluronic ALA-L with tailored degradation rate, was tested as an encapsulation vehicle for osteoblastic cells. UV cross-linking of the modified polymer results in a stable hydrogel with a slower degradation rate. Toxicological screening showed no adverse effects of the modified Pluronic ALA-L on the cell viability. Moreover, high viability of embedded cells in the cross-linked Pluronic ALA-L was observed with life/death fluorescent staining during a 7-day-culture period. Cells were also cultured on macroporous, cross-linked gelatin microbeads, called CultiSpher-S-(R) carriers, and encapsulated into the modified cross-linked hydrogel. Also, in this situation, good cell proliferation and migration could be observed in vitro. Preliminary in vivo tests have shown the formation of new bone starting from the injected pre-loaded CultiSpher-S-(R) carriers.},
  author       = {Lippens, Evi and Swennen, Ives and Giron{\`e}s Molera, Jordi and Declercq, Heidi and Vertenten, Geert and Vlaminck, Lieven and Gasthuys, Frank and Schacht, Etienne and Cornelissen, Maria},
  issn         = {0885-3282},
  journal      = {JOURNAL OF BIOMATERIALS APPLICATIONS},
  keyword      = {BONE,MICROCARRIERS,IN-VITRO,STEM-CELLS,DRUG-DELIVERY,BIOMEDICAL APPLICATIONS,THERMORESPONSIVE HYDROGELS,PHARMACEUTICAL FORMULATIONS,bone formation,Pluronic,cell encapsulation,hydrogel,SCAFFOLDS,CultiSpher,CARTILAGE},
  language     = {eng},
  number       = {7},
  pages        = {828--839},
  title        = {Cell survival and proliferation after encapsulation in a chemically modified Pluronic{\textregistered} F127 hydrogel},
  url          = {http://dx.doi.org/10.1177/0885328211427774},
  volume       = {27},
  year         = {2013},
}

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